FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length75
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34.10958904109589No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA22.73972602739726No Hit
ACTATGGGATTCTGGTTGATCCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACA11.36986301369863No Hit
CAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAG11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGGAGACAACATGAGCAAGTATAAAAAACACATCTC11.36986301369863No Hit
GCTCGATGCGCATGCCCCCCGCCTTCAGGGCCTCAAAAAAGCCGAAGCCCAGACGTTGGGAAAGGGTTCCAAGGG11.36986301369863No Hit
TATTAACTAAAAAGAAAAAAAAAAAAAAAAAAAAAACGAAAAGCGAGAAAAACCATGAGCAACCAACCGTAGGAG11.36986301369863No Hit
GCCCTAGGCGTAAGGGCCATGATGACCTGACGTCGTCCCCGCCTTCCTCACTGCTTGCGCAGGCAGTCTGTCTAG11.36986301369863No Hit
ATATAATTATTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAACACACAATAAAATACAACAAACCAAAAGGTACC11.36986301369863No Hit
ACTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAATACCGACCCACTGAAAAAAAAACGAGAAACAACAAAGTGG11.36986301369863No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTTTAATAAGAACCACCTAATTAAAAAACATAAAAAGTGCACTA11.36986301369863No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTTGATGGTAGTATCTAGGACTACCATGG11.36986301369863No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACAAGCCCACCAGTAGATAATAAAAAAAAAGTATAC11.36986301369863No Hit
ATCTACAGCCCACTCGATGATCGCGAGAATCGCTCGGCGGAAAATGGTACGTCCAATGCCGAGGAGTGGGGCCGC11.36986301369863No Hit
AAAACGAACAACAAAATGAAAAACTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACCAACCAAACCC11.36986301369863No Hit
TCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAAT11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAA11.36986301369863No Hit
ATAAACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAGAAAAATTAAAACAAAGAAAGAAAAAAACGAA11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGACAACAACAGCGCCACGCAAAACAAGAACAAAACCCGAAAAC11.36986301369863No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACAGCACCACACCCAGGACGAAAACAGAGGCTTCGCC11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAGCAATTAAATAAATATACCATGCAAAGCAAATAA11.36986301369863No Hit
CCTCAAGTGATGATTATTGGAGGTAGAGCACTGTTTGGACGGGCCCCTCTCGGTTACCGAATTCAGACAAACTCC11.36986301369863No Hit
GACCGGAGGCCACCGCGGCCTCGCTTAATCCGGTCACAGGTATTATAGTTGGATCCGGGAACGGGGGGCAGCTTC11.36986301369863No Hit
GTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTAGTCACTACCTCCCCGAGCTG11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATCCCACAGCGAAATATAAAAAATAA11.36986301369863No Hit
TCCCAAAGGAGAACACCCTACGGGTGGAAAACCTTGTCCTGGGAGCAAGACTTGTTTGGCGTTACGGTACGAATA11.36986301369863No Hit
CAACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACACATAACAGATAACACAAAACAACGCAGCAGG11.36986301369863No Hit
ACATATATCTGCTAGCAAAAAAAAAAAAAAAAAAAACATAATAAAACCCAGATTCAAAATAAAAAATAAAAACCA11.36986301369863No Hit
CGTCGTAGCGGCGCTCGAGAGCTGCAGGCCGCGGTCGTTGGCGCGTTGGGCGGCGACGCGGGCGCCGGGGTCGAC11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGCACAAAAGGCAAAACGAAAATAAGA11.36986301369863No Hit
ATCAGATACCGCCCTAGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACCTTTATGGCTCTCTC11.36986301369863No Hit
ACAAGGATAACTCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAGGCACATCTGCTAATAA11.36986301369863No Hit
CAACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCCACCACCCCCACAGAAACCAACAAAACCACAAA11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGCACGCCCCCCGAAAGAAAAAACACCCCGC11.36986301369863No Hit
ACATAAACTATACATAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGACCAAATACAATAAAAAAAAAAAACAA11.36986301369863No Hit
AAAATAAACTAATAAAATCACGCAAAAAATGCATAAATAATCTATGAATCAAAAAAAAAAAAAAAAAAAAAAAAA11.36986301369863No Hit
CTTCCGATCTTGCTGGGGATTCTGGTTGATCCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATG11.36986301369863No Hit
TCCCTAAAGAGATCGGAAGAGCACACGTCTCCCAGCCCAGATCGGAAGCTGTCTCTTATACACATCTCCGAGCCC11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCATACAAATCCCAAACTAGAAAGTTCCTCTTAACATCACACATTTGGTAATCAACCAAAAAAAAAAAAAAAAAA11.36986301369863No Hit
CATAGACGGTGATCTTCTCGATGTCCTGCAGCTTGGCGTTGCTTACGGACAGGTAGGTGACCACCGACTCGGTCT11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAACAAATAAAAACTCAACCCCCACCAGGCA11.36986301369863No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCATTATAAGGAAACTAGCTTCTTAAATGGACAAATTGCGTCTA11.36986301369863No Hit
GTCGGGATCGTCGGGAGCCGTGTCGAAAATCGGAAAGGATGGCTTCCAGGTCTGCTTGGATGTGTCGCACTTCAA11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGATATAAAAAAAA11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAACGCAAGAAACCGCGCTAATTATCACAAATAACCACCACACCGAGACCA11.36986301369863No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGAAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGATA11.36986301369863No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAACGAGAGGTGAAATTAATAAACATACGAAAA11.36986301369863No Hit
ATTAATAACCATAAACTAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACACAAGAATAAATAAA11.36986301369863No Hit
GGGGAGACGTGTGCTCTTCCGATCTGGAAGGGGAGACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGCTGTCTCTTAT11.36986301369863No Hit
GCAATGATATCAACTGGCGTATCGTACTTCTGGAATAGATTCGGGGGCAGTGAGTCCACCACCTGCATGTCGTGC11.36986301369863No Hit
CTTCCGATCTAACCAGGGAGTCTGGGTGATCCTGCCAGGAGTTATATGCTTGTCTCAAAGATTAAGCCCTGCCCT11.36986301369863No Hit
CTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCT11.36986301369863No Hit
ACACCAAATAGAGAAGTGCAACAAGACACTCTCAACAAGCATGCGCAGATATTGGTAAGATGCTTTAATGGAGCA11.36986301369863No Hit
GATGTAAGTGGGTGTGAGTTTGAGTGTTTGCTTGAGCTGCGCGGCTCCAGAAAAAGTTAGCTAAGCTGATCGGGT11.36986301369863No Hit
GCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATCTAAGGAACCATAACTGATATAAT11.36986301369863No Hit
CTAAAACGACTCCACATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATTTTAAATTAAAAAA11.36986301369863No Hit
CTATTGAATATATTTGAGTCTCGTTCGTGATCGGAATTAACCAGAAAAATAACTCCACTAACTAAGAAATTCAAT11.36986301369863No Hit
GATTTATGCAACTTTAATTTTGGAGCAATGCTTGGGCTAAGAAGTTTAGCATTTAGGTGTAAACAGGTACAATAC11.36986301369863No Hit
ACAAAAAAAACAAAAGAAAATGCAATAAAAGAAGAACAAACAAAAACACATCAAAAAAAAAAAAAAAAAAAAAAA11.36986301369863No Hit
GCCATTTGGTTTTTGGTTTGGATTGCCCTGATGCTGTTGGGGCACATACATGCCTGTCTCTTATACACATCTCCG11.36986301369863No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATG11.36986301369863No Hit
AATAAATGACTCTCAAATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATAAAAA11.36986301369863No Hit
TATTTTGACTGTGCAAAGGTAGCATAATCATTAGTCTTTTAATTGAAGGCTGGAATGAATGGCTGTCTCTTATAC11.36986301369863No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTA11.36986301369863No Hit
CGAAAGATAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAACGCGTCAATATAAAAAAAAGAAGGCA11.36986301369863No Hit
CCTTAGATGTCCTGGGCTGAACGCGCGCTAAAATGAAATTATCAACGTGTATTTCATAGACAGATAGGTCAGTGT11.36986301369863No Hit
AACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAAGGCGATCAGA11.36986301369863No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAGAGCCCAAGGCTCCCCTGTCGGTTATACAAATAGCCGAGCC11.36986301369863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACACACCACAAAAAAAAAAAAAACAAGAA11.36986301369863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC50.069.06
GCGGGCG50.069.058
GGGTCGA50.069.068
CGGGGTC50.069.066
GGCGCTC50.069.010
GTCGTTG50.069.034
GCGACGC50.069.053
GGCGCGT50.069.040
GGCGCCG50.069.061
GTCGTAG50.069.02
GGCCGCG50.069.027
GCAGGCC50.069.024
CGCTCGA50.069.012
GCGGCGC50.069.08
GCGGCGA50.069.050
GCGGTCG50.069.031
GCGCGTT50.069.041
GAGCTGC50.069.019
GGCGACG50.069.052
CGTAGCG50.069.04