FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length75
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67.894736842105263No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCATGCTTCAAATGCCTGAATATTCTGTTCATGGGATAATG11.3157894736842104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAGAAAGGCATATAAATAATAAACAGGTGATAAA11.3157894736842104No Hit
TTGCAACCATAATTAACACCGAGCCCAAAACCTAAGGTTTCAAGGTAAGCGACTCAAAGAGCAATAAAATTACCT11.3157894736842104No Hit
AAACAATAACACGTATTTCAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCTAAA11.3157894736842104No Hit
CCGTTGAGCGATGGCCCACCCACGCGGGACCACCGGATCACTATGACAGACTTTCGTCTCTGCTCGACATGTCCG11.3157894736842104No Hit
AATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAAC11.3157894736842104No Hit
AAAAAACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACCACAATACAATAAAAAAAAAAA11.3157894736842104No Hit
GTTGCAACCATGGTAGTACTAGATACTACCATAAAAAGTTTATAGGGCAGACATTTGAAAGATCTGTAGTCAGTA11.3157894736842104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAACAACGAAATGCAACCTGGCAAAGATAAACAAGAGAACACCGGCTGGCAGG11.3157894736842104No Hit
GTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATCTAAGTAAC11.3157894736842104No Hit
CAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACCTGTCTCTTAT11.3157894736842104No Hit
ATTGTGCGCCGCACTGAGGAGGTGGCATGCCCCATTACGGGGCGACCTCAACTGCGCTCCGTCGAGTACATTGAG11.3157894736842104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA11.3157894736842104No Hit
TATTGTGAGCTCGATGAGTAGGGCGGGACACGGGGTATCCTGACTGAATATGGGTGGACGATCCTCCAAGGCTAA11.3157894736842104No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGATAAAGGGCGTGCTAATCTGCGATAAGCGCCGGTAAGGTGAT11.3157894736842104No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTATTATAAAATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.3157894736842104No Hit
TCAATAACCAGCCACATTGGAAAATAATTAATATAATACATCCATTATAACCGTCTCTTATACACAACTCCGAGC11.3157894736842104No Hit
AAAAAAGACACAGCCAAAAAGCAACAACATAATGATAATGGCATGAAAAAAACACACACACAACAAAAACAGCCA11.3157894736842104No Hit
GGCAGGACCAACGCTGAGGTGCTAACGCAAGTGGAGCACGGCTAGCGAATGCCGCAACCTCCCAACTGCGAGCCG11.3157894736842104No Hit
CCATTGGAGTTTCTGAACGTGAACTCACTTAGTGATTGTAGGCAATAGAATCCGGCAAAGAAGCAAGTCTGATGC11.3157894736842104No Hit
AAATTAAAAATATAATAACAACAGATAAAAAAAAAACATTAAAAAATCAAATTAAACTATATATAATAAACAACT11.3157894736842104No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAATTATAAAAAAAACAGATAACAAT11.3157894736842104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAATTA11.3157894736842104No Hit
ACAAAGAAAATCACAGAATAACATAGAAAATCACAGAATAACATATAATAACATATGTCTCTTATACACATCTAC11.3157894736842104No Hit
CATTAACGTATAATAATAACCACAATAGAAAGTTGTACTACCCGTAGGTAGAAAAAAATAAAAAACGAAAGTAAT11.3157894736842104No Hit
ATACTATAGTTAAGCAAGGAATCTCCGGGTCCGCGAATAATAGCGAAAGTTTTGAGGACAACTTCACGCCCTTGC11.3157894736842104No Hit
AAAATAAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACGCCAGAACCACAAACCACAAAA11.3157894736842104No Hit
CAATATCTCTATATCGAGTGCATGTAGAAGAATCTTGTCACCAACTCGCACACACTCCAATGTCCCAAATGAAAC11.3157894736842104No Hit
GCGTCTATTAATCATTCCCTCGTGATCTGAAAACCAATGAAAGCCTGTCTCTTATACACATCTCCGAGCCCACGA11.3157894736842104No Hit
ATCAAAAACTATTACATATAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTGAATATTAAAATAATAAAA11.3157894736842104No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATATGTCGTCGGTACAAGACCATACGATA11.3157894736842104No Hit
TATAAACACAGACCGCATCCGGACTGGCGAATTGCCGAAAAAGCGAGACGAGAGGAGAAAGTGAGGGTTCCAGTC11.3157894736842104No Hit
GAGCACCCCGTCTCAGATCGGAAGAGCACACGTCTCCCTGTTCAGATCGGAAGAGCACACGCTGTCTCTTATACA11.3157894736842104Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTAGTAGCTGTTTCCCAATGAAGCACGCTGTTTATTTGAAATTAAAAAAGAACTGGATGCATATATTTCGTCTG11.3157894736842104No Hit
CCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGTAAATTCTGTCTCTTATACAC11.3157894736842104No Hit
GTGTTTGCACAGTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGGCTTCAGAGAAAAAAA11.3157894736842104No Hit
GTACATTAGTCCTTCCAGCCAGACTCGGTCAAATGCTTGTGCAACATCTAAGACTGTCTCTTATACACATCTCCG11.3157894736842104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAGAAAATAACAAT11.3157894736842104No Hit
GTTTTAGCCTAATAAAAGCACATGTCGCATAAGGTTCTTGTTTTAATTGCATGTATTAGATCTGTCTCTTATACA11.3157894736842104No Hit
TCTTTAAGTTGTCCGCGAGATTGTTTTGCTGCAGCGCGAAGGCGCTCGACCGGATGGTGAGGAACATGGACTCCC11.3157894736842104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCAAATAAAAAAACAGCTGACGCACACAGAAAGGCTA11.3157894736842104No Hit
GTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTAATTAATCCAGAACGAAAGTTATAGGTTAGA11.3157894736842104No Hit
AGACCTCTGTTCTGCTTTCATTGGTTTCCAGATCAAGAGGTAATGATTAATAGAACCATATATGGGCCATTAGTA11.3157894736842104No Hit
CGTATAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAATAATCTAATAACAAAATACAATA11.3157894736842104No Hit
GCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCGGCCCACAATAACACTCTGTCTCTTATACACATCTCCG11.3157894736842104No Hit
GTTTCTCAGGCTCCCTCTCCGGAATCGACCCCTTAATCCCAGTTACCCGTTGCAACCATGGTAGGCCTCTATCCT11.3157894736842104No Hit
GAATTTCACCTCTGACAATTGAATACTGATTCCCCCGACTGTCCCTATTAATCATTACGGCGGTCCTAGAACTGT11.3157894736842104No Hit
AAAAAGAAATAGTCAAAAAACAACTAACAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAAACTA11.3157894736842104No Hit
ACAAAAAAAAAATCTAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAATAAAAAAAAAAAA11.3157894736842104No Hit
GCACTCAAAATTGATCAAAGTAATAGAACCTACACACAAAAAGAATACATAAACAGAAAGAATCATATAATAATA11.3157894736842104No Hit
CCCCCACCCCAGCGCCACGCTTACCGTGCCAGGCCTCTCCCCCACGCCGGGACGCCGTCCCAGCCGCTGCGGTAT11.3157894736842104No Hit
AGCTCAACGAGGACGATAGACTACTGCAACATCTGCAAAACTGTCTCTTATAAACATAACCGATCAAACAAAACA11.3157894736842104No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAAC11.3157894736842104No Hit
CAATAACACTCGTTTAAGAGCACTAATGCAGGTTTTTAAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11.3157894736842104No Hit
CTACCAAAAAAAAAAAAAAAAAAATAATAAAAATAACTGCACAATATCGGCAACAGATCCGATAATAATTAAACA11.3157894736842104No Hit
GTAGAGAGCGGCGCGCGTCAAAATCGGTCCGCCCATGAAGCAAATGGGTGCGATCCCGGCGCCCAGCCTGTCTCT11.3157894736842104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAACATAGATAAAAAAAAAAAAAAA11.3157894736842104No Hit
AGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAT11.3157894736842104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTCACAAATAAAAATAAAAAAAAACAAAACACACAAAA11.3157894736842104No Hit
CTTCCGATCTACGCCGGGTGAAACCGAGCGCGCACCCGAATGCCGAAAATCAAGTATACGTCGCTACGATCGCTT11.3157894736842104No Hit
GCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGG11.3157894736842104No Hit
AAAAAAAAAAAACATCTAAACAAACATAAATTTTACCATAATAAACTAACAAATACAAAAACATTAAACTATAAA11.3157894736842104No Hit
AGCGTATATTAAGTTGTTGCGGTTAAAACGTACGTAGTTGAACTTGTGCTTCATACGATGTCTCTTATACACATC11.3157894736842104No Hit
GTTATCTAGAGTTCAACCAAAATAACGATCTTGCGATCGCTTGGTTTTATCATAATAAAAGACAATCTCTTATAC11.3157894736842104No Hit
TGGTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAC11.3157894736842104No Hit
TCATATCAAAGAACTTAAACATCACCACATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATAAAAA11.3157894736842104No Hit
GAACACATAAGAAATCACAATCGAACTTACAATAAAATAATATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.3157894736842104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAAAACCAAATGATAGACAAGGCAAGGCCGCACGACCAGCCA11.3157894736842104No Hit
TGTACATTCCGCTTTATTCGTTCATAATCTTTTATGATTTCTCATTTCCTGGCGCGTTTAATTTGGTGAGGGGGG11.3157894736842104No Hit
CTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCATGGTAGCTGTCTCTTATACACATCTCCGAGCCC11.3157894736842104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGG50.069.030
CTTTTAT50.069.044
ATGGGAC50.069.032
AAACCAA50.069.058
CATGAAC50.069.022
ACATGTG50.069.037
AAACATG50.069.019
ACATGCA50.069.08
TGCAATT50.069.011
ACATGAA50.069.021
ATTAGGC50.069.049
GCGATCG50.069.065
TAAAACC50.069.056
TAAAACA50.069.017
AGCTAAT50.069.01
TTATTAG50.069.047
GGACATG50.069.035
AATTAAA50.069.014
GATCGCA50.069.067
AACCTTA50.069.026