FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length75
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33.79746835443038No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGCTGTCTCTTATACACATCTCCGAGCCCACGA22.5316455696202533No Hit
GTCTTCGATCATTGGCAGGTGTTGCCTGGCGATCCGAGCGAGCCCTCAAGCAAGCCCTATGCCATCGTCCAGGAT11.2658227848101267No Hit
TTTCGGAAGAGCACCCCGCACCAGATCGGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGGAGTAA11.2658227848101267No Hit
GCACAGTGGAAACATTGGCTTCGAAGACATTCTGGCCATCGCCAGCGTGATGAGTACCAGGTCCATGGCCCGTGA11.2658227848101267No Hit
CCCATCTACAGTCTTTGCTTCGACAATATCTTCAATTAGTGTTTGCCACTTTTTAACCATAGACCTGTACTTGTA11.2658227848101267No Hit
CTTCCGATCTTACTAGGGAGACGTGTGCTCATCCGATCTTACTACAGAGACGTGACCTCGTCACTAGTTAATAGG11.2658227848101267No Hit
ATCGATATCCACGTGGAAGAAATGGTCGCCCAGCGAGTCAGGCCCGGGTGGAAGAATCTCCGGGTCCGTTTGCGG11.2658227848101267No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTT11.2658227848101267No Hit
GAATAAGCCCCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGG11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACACCCACCACGAACCCATCAAAAAAAAGAAGGCGG11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAAAAAAAACAGAAAAAAAAAAAACCA11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCACATGCAGAACATCCACAATAAGAAGACT11.2658227848101267No Hit
CCCTTGAAAAGTCAAGCGTATTGTAAAAGTAAAAAGTCAGTATTCGATCCGGTGCAATTAAATCGAGATCCAATA11.2658227848101267No Hit
CTATGGTTTATATTGAACCGTTGCCGGAGAAAGCTCAGTAATAGAAATAGGTGAAGAAAATGCGATTGGCAGTAT11.2658227848101267No Hit
GCCAACGACCATACCACGCTGAATACATCGGTTCTCGTCCGATCACCGAAATTAAGCCTGTCTCTTATACACATC11.2658227848101267No Hit
CATCAAAACTTAATTATTAATATAATAATTTTTTTTTAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2658227848101267No Hit
GATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG11.2658227848101267RNA PCR Primer, Index 47 (96% over 29bp)
ATCTCACACCGTTCGTTTCCAACGAGCTGCTGTACAAGTCTTTCGAAATCTTTGGTCCCATCGAGAGGGCCAGCA11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAACAAGCAGCTGTCTCTAAAAAACAACTCCCAC11.2658227848101267No Hit
ATAAAAAATTAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2658227848101267No Hit
AGCCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCT11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGCAATCACAAA11.2658227848101267No Hit
GATCAGGCCCGTACATCCCGACCTATCTTTTTCAACCTTCGATGTCGGCTCAGGCTATCATGACTCTTATACACA11.2658227848101267No Hit
CGCTTGGCGGCATAACGTCCGGCGGAATGGGGAAGATAGCCCCCAAACTTCTCCTTCACCGAGATGTAATCCACC11.2658227848101267No Hit
AAACAAATCTGGTGAACAAAGTTAACAAATTGAGGTAATTAACAAACGACAAATTAAAGCAGCAACCAATACTAT11.2658227848101267No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTATCC11.2658227848101267No Hit
CAAACGAACCATAACTGATATAATGACCCTTTTGCCGTTTAACTTATAATTCGAGTGTACTTAGACATGCATGGC11.2658227848101267No Hit
CTTCCGATCTCCGTTGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGGAAAAGAATTT11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAGAGGAACCGATGATAAATATAATTGGAATAACCGCC11.2658227848101267No Hit
CGACACAGGTGGACTGGTAGAGTATACCAAGGCGCTTGAGAGAACGATGCTGAAGGAACTCGGCAATTTGCCTCC11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAGAAAGATGAATAAACATAA11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATATCTAAAACACAACTAAGAAAAAAATAAAAACAA11.2658227848101267No Hit
GATCGGAAGAGCACACGTCCCCTCATTAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTA11.2658227848101267Illumina PCR Primer Index 10 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCCTAATAAAATAAAATAAAAA11.2658227848101267No Hit
TCCCGGGGGAGGTTCACCGTAGAGGAGATGCTTTGGTCCACGTGGCGTTGTAAGGTGGCCTGGAGGTGTACCCTC11.2658227848101267No Hit
CCTTAGACGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTAGACCTGTCTCTTATACA11.2658227848101267No Hit
ACCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACTTTTATGGCTCTCTCAGTCGCTTCCCGGGAAACCAAA11.2658227848101267No Hit
GTCCGACACCGATCCGCAATCCCAGCTCAACCAATCAATTGACTGTCTCTTATACACATCTCCGAGCCCACGTGA11.2658227848101267No Hit
GATCGGAAGAGCACACGTCTCCCGGCCTAGATCGGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCG11.2658227848101267No Hit
CTAAATGGAACTCTTGATTGATGCTAACACCGAAACGAAAAACGCCGACTGGGTAGCCCTAAAATGGTGGCCCTA11.2658227848101267No Hit
CGTTTGCGGCGTTCCTCAAACCGACACGCGCTCAACCACTACGCCAACAATGTAATCGGGTATGTCCATCTAGAC11.2658227848101267No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCA11.2658227848101267No Hit
TCTTAGTGCAGTGGCTCAATCCTAGTTCACTGTAGCCTGGAACTCCTGGACTCAGGTGATCCTCCTGCCCTGTCT11.2658227848101267No Hit
CAACAAATCCTCTCGCTGACTGCCGCCGAGGCCGCTGTCGCGGCGGTTGGCGGCACGGCGTTGGGGACGGTGGCG11.2658227848101267No Hit
CTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACATGCATGGCTTAATCTTTGAGAC11.2658227848101267No Hit
ATATAAGGAAGGATCTTCAGCCAGATCGCGGTCATGCCAGCACCTTCAATGCCTTGTTGTACAACGCCTGGCCTG11.2658227848101267No Hit
GTTAGAACTAGCGCGCTATCAGATCGCCTTCGAAACTCTAACTCTTTATCTTATACACATCCCGAGCCCAACGAG11.2658227848101267No Hit
ATATTATGATTCGCTTTTTTATTGTTTCATAACTCTCTGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11.2658227848101267No Hit
GTATGCGAGTGAGAATGCAGGCATGAGTAGCAAATGACGGGTGAGAAACCCGTCCGCCGGATGACTAAGGGTTCC11.2658227848101267No Hit
AAAAAAGGATCGGAAGAGCACACGTCTCCCGAAGTAGATCGGAAGCTGTCTCTTATACACATCTCCGAGCCCACG11.2658227848101267Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTCACCGCTAGGCGGGACGCAGTCCGTCAGGTACTGGTTGTCCCGCTGGGCACGAGGAGTGTTCACCACCAGGC11.2658227848101267No Hit
AACAAGAACTATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAACAAGACAATTAAAAAAAAAACC11.2658227848101267No Hit
ACCATGGAAGTCTTTCGCAGAGAGATATGGTAGATGGGCTAGAAGAGCATGACATATACTGTTGTGTCGATATTT11.2658227848101267No Hit
TCTCGGAAGAGCACACGTCTCGGAAGCTGTCTCGTATAGGCATCTCACACGACTCGAGAGGCCAAGGATAGGCGA11.2658227848101267No Hit
ACCATACTTACCCCGGAGCCCCAAAGCTTTGGTTTCCCGGGAAGCGACTGAGAGAGCCATACAAGTAGCTACACC11.2658227848101267No Hit
CTTCTTTTTTTTTGTTCAAGTTTTTACTTCCATTTTTTTTTTGTTTTTTTTTTTTTTATTTTTTTATAGATATAA11.2658227848101267No Hit
CCTTCGCCGTCATCAAGCCCCTCCTCACCCGGCCCGCCGAGGCGGTCCGGGGGCCCGGGGTCTGTCTCTTATACA11.2658227848101267No Hit
AACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGT11.2658227848101267No Hit
ATGCAAAACAAATCGACAAGCAACGGTGGAACGGTCAGCGATCCAGAGCCCCGTCATCCGCTTCCTCCGCAGCTG11.2658227848101267No Hit
ACCATTGCCGCACCCGTGGCCAATCTGATGATGCCCTCGCCGTCGGGCAAGTCCAATTCTTCGACGGGCTCCACA11.2658227848101267No Hit
GTTCAGGCCCTTGGCCAGCGCCGCGCGGACATATTCCATACCGTCTGTCTCTTATACACATCTCCGAGCCCACGA11.2658227848101267No Hit
CCAGGATGCCCGGGCTCGGGTTGCCGCGCAGTTGACCAGCGACTTCATGATTGTTGTCCAAGCGCTTGGCCCGAC11.2658227848101267No Hit
CCGCCCTAGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA11.2658227848101267No Hit
ACAAAAATCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAACCACACAAATAAAACAAAAAATAAAAGAAC11.2658227848101267No Hit
AAATAAGGGGTCCTGCGCCGAAAATGTAACGGGGCTCAAACCACGAGCCGAAGCTTCGGATGCACTCCTTCGGGG11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCGCCACATATCGCATTAAAAAAAAAATGCGACAAAAG11.2658227848101267No Hit
GTTATCCAAGTAACTGTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATT11.2658227848101267No Hit
CATCAAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCCGCGAGCTAGTGACGAAAAATAACAATACA11.2658227848101267No Hit
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCAAATACATGCAATTAAAAAATCAACAT11.2658227848101267No Hit
CTTTGCCTTCTGGCTGCCGCTGGCGGCGGCCCTGGCTTGTGTCCGACGGGCATCGCGAATCACGTCGATCTGCGT11.2658227848101267No Hit
GTAATTGGAATGAGTACACTTTAAATCTTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTTCCAGCAGACGCGT11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATAAATAGTAATAATAATAAAGAAAA11.2658227848101267No Hit
TTTTGACATCGAGCTCCTTGGCGTTCGGCTTGTCCTCGACCAGGCGCGGCAGCGAAATCGAGCCGATGCCGTCAT11.2658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAATTGAAATAAAAAAAACA11.2658227848101267No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA11.2658227848101267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAA50.069.053
CCTAATA50.069.055
GCCTAAT50.069.054
AAAAGCC50.069.050
ATAAAAA50.069.069
CTAATAA50.069.056
TAATAAA50.069.057
AAAGCCT50.069.051
AAAAAGC50.069.049
AAAAAAG50.069.048
AAGCCTA50.069.052
TAAAATA100.034.560
AAATAAA100.034.562
ATAAAAT100.034.559
AAAATAA100.034.561
AATAAAA150.023.058
Produced by FastQC (version 0.11.2)