FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length75
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2015.503875968992247No Hit
AAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGAACGAAGTATCTAGGACAACAAACGTTGCAACC10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCGCGAAGGAATTG10.7751937984496124No Hit
GTAATCTAAAGATCTGTCAAGGCATGATCAAACGAAAAAAAAAAAAAAAAAAAAAAAACAAAAATATTAAACAAA10.7751937984496124No Hit
TAACCATAACTCATATAATGAGACTATTCCGCTTTCAATATTAAATCGTGTGTACTTAGACATGAAAACCTAAAA10.7751937984496124No Hit
AACACAATTAAAGAAAAAAAAGATGCCTCTATCATGGGCCCACTCCGCACACTCCTCCATCGTTAACGTAGACAA10.7751937984496124No Hit
GTTCAGCACCATGGGGCCGCACTCGCGCAGGTCCACCTCGTAGGTCTGCATGTACGGCTTCTCGCCGGCGTTGTC10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCACCCAGACAAATAGAACACAAAAGAAAGGAGAGACGA10.7751937984496124No Hit
ACTAAACAAAAAACACAAAATCGACATAAAGTCACAACACCAACATACCACCATCAGGAATTCCACTGACATCAA10.7751937984496124No Hit
GATCTAGTGTGGGTGTGACAGGACACTTTGCGTCAGCAGCAGCAGCAGCAGACCGAAAAGTAGCAACAAAGATGG10.7751937984496124No Hit
GGCAAACAATTCCTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAACAACTAAAA10.7751937984496124No Hit
CCGATATATAGTGCGATGTGGGGACGGAGAAGGTTAGCTCAGCCGGGTGTTGGATGTCCCGGTTCAAGCGTGTAG10.7751937984496124No Hit
GCCCCGAACTGGTGGCACCCGTGGATAGATGTTAACTCCGGAGGCGATGCACCTCCAGCCGGGGGGACGATGCTC10.7751937984496124No Hit
AAAAAAAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAAAAAAAAATAAAAACAAAAAAAGAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAATAAATAAAAATAAAAATAAAAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAGGACCACCCCCAATAAAAAAAATAAACAAAAAC10.7751937984496124No Hit
ACATACCAACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATGCCGACCTCTTGAATCTAAAAT10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGAACAACACGTATGAAGCACAAGTT10.7751937984496124No Hit
AAAAAACAGTTCAAATTATAGAGCAATTAAATTCAAATAACAAATCCAAATAAAGCCACATTTTCATATATATCT10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAGACCCAGACCAATAAAAAACAAATCACCAACA10.7751937984496124No Hit
CTATTACAACTACTCAAACTAAGGCTACCTTAACCGCTACAACTAACGCCTATATTTAGTCTGTCTCTTATACAC10.7751937984496124No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACCCCAAAACTGCTAATATAAAAAATTACACTGACACT10.7751937984496124No Hit
GCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGCGTGGGTAATTTTCGCGCCT10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGTGCCCCTCAAAATAAAAAACAAACAACAAAAAACAAAAAAA10.7751937984496124No Hit
ATAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGACCACCAGACAAGAACAAAAAATCTGCAA10.7751937984496124No Hit
AACTTGTACTAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAGACTCTTAAACAAAAAAACGAGAC10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAGGGCCCGGGAGGGTTTCAAAAGGACATGAGAAGGGTA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAA10.7751937984496124No Hit
AAACACACCCACACCATTCCTCTTCACATATATCAGCAGTACACAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCCTCACTCATATACAAATAAAAAAGAAAAAGAGA10.7751937984496124No Hit
AAAAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCACAAAAAAAACGTGAGTAATTAAAAAATATCT10.7751937984496124No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA10.7751937984496124No Hit
GAAAAAAACTAAGTAAAAATAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAAAATCAGCAAGTACTTTT10.7751937984496124No Hit
ATATAACTATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCA10.7751937984496124No Hit
GTCTTATTTCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAG10.7751937984496124No Hit
GAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAA10.7751937984496124No Hit
ATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATG10.7751937984496124No Hit
AGTTAGAGGTTCGAAGGCGATCAGATACCGCCCTAGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGAGTAG10.7751937984496124No Hit
CTCCCACAAAACGCTCACGGCGATGGTCTACTATCAGCAGCAACCGCAGCAGCTGTCTCTTATACACATCTCCGA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAATAAAAAAAGAAAT10.7751937984496124No Hit
CAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACAACCCAAAAAAGATGAAAAAAAAAAAAAC10.7751937984496124No Hit
AACACACACATCATCATCGGAGGAAGAATAAAACGCATATACAAAGCAATAAGACAGACAACTCAAACACAACAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAACAGAAACACATATAATAGAAAAAG10.7751937984496124No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCCAATACGGAGAGGCAGCAATAAAACAATAAAAAACACATC10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAACCAACAAACCTGTATAAAAAACACAACTCCCACCC10.7751937984496124No Hit
TCGATAGATAGCTCACTTGCTTGCCCATTTGGGGCAGGACTCGCGCCGTTTGTCTGGTCACGGATTGCATCTGTC10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGGCAGCGGGCCGCGGGGGGGGGGAGG10.7751937984496124No Hit
AAAAAAAGAAGCAATACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAATAAAAAAAAAAAAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAACTAAAAAAAAACTAAATAAAACACCACTCGGA10.7751937984496124No Hit
ATATTGACTTAATTACAAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAACCATAAAAGATAA10.7751937984496124No Hit
GCTTAAAGCAGGCTTCAAATGCATGAATATTCTGTGCATCGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAATAAAGAAGGAACAGGCGACAACGAA10.7751937984496124No Hit
ACCTCACTGAGCTCGTAATAAAATTTCCAATCAAACTGGGTTCAAAAATGGAAATTAAATTTTTTGGCCATATTT10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAATAATCTTATGCAACACCCGAAAAAAAAAACAAAAAAAGACCAAGGAGA10.7751937984496124No Hit
TCCCACGCCAAGGGAATAGTCCTGGAGAAGGTCGGCGTCGAGGCCAAGCAGCCTAACTCTGCCATCAGCAAGTGC10.7751937984496124No Hit
AGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGACCTATCACTGTCTCTTATACACATCTCCGAGCGC10.7751937984496124No Hit
ATAAAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTTAATTAAAAAAAAAAAAA10.7751937984496124No Hit
GTTTTAAGCAGGAGGTTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACG10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAATA10.7751937984496124No Hit
TAATTCTACCGTAGAAATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAATCAACAAAGAAATAT10.7751937984496124No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAACTG10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGCAAGCGGAAAAAAAAAAAAAAAGCCGACCCACGGAAACAG10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAA10.7751937984496124No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCT10.7751937984496124No Hit
AATCAAAACAGCAAAAATCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAACAAACTCTAAA10.7751937984496124No Hit
GTCGTTGCTCCAGCTGCCGCGGGTCAGGCAGTTCAGCAGCAGGCCGCTGGAGCGGTAGCGGTAACGGGTGTGGCC10.7751937984496124No Hit
CTCCTCCCTTCTCAGACTGAACTACCGACCATAGCGGGGCCATCGTTGCTGCAAACGTAGCAACTGGAGCGGTGG10.7751937984496124No Hit
AACAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATCAACCGAACCCATAGATAAAGCCAAAAAAA10.7751937984496124No Hit
CCCCAAACCAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG10.7751937984496124Illumina PCR Primer Index 10 (96% over 29bp)
AAATTAATATATTAATATGTAAAAAAAAAAAAAAAAAAAAAATAAGTAAAATTAAAAAATTTTTAATGAGAGAAA10.7751937984496124No Hit
AAAAAAAACAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATTAAAATAAAAAAAAA10.7751937984496124No Hit
GTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGCTGTCTCTTATACAC10.7751937984496124No Hit
CCAGAAGAGCAGGTCGTCCATGTTCTCGAGCGTGCACTGGGCGAAGACGGCTCCCGCATCCCGGCCGTGTTGGTG10.7751937984496124No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTAACCGTATGAAGCACAAGTTCAACTACGAACTTTTTAACCGCA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAAAAA10.7751937984496124No Hit
TCCACGCAAATAATTGCGCTTACGCTCTAAAGTGCTGATTTTTCGGTAGCAGGATGTCTCATATACACATCTCCT10.7751937984496124No Hit
GAAAAACAAAATAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAAATTTAAAAAAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACCCACCCAAAATAAAAAAAAAAAAAA10.7751937984496124No Hit
TGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGC10.7751937984496124No Hit
AAAAAAAGATCAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAACACCATGATATATAGAGTTAAAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAGCCGA10.7751937984496124No Hit
GTCCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGA10.7751937984496124No Hit
ATTCAGATCACACAAGCCTCTGAAATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACTAAAAACGTACGTA10.7751937984496124No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTT10.7751937984496124No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAAGGAAACATCAAAA10.7751937984496124No Hit
ATTCCGAAAAAAAAAAAAAAAAAAAAAAATAATACAAATAGACCGGATCGAAGTCCACCAGGTCGAAGCGGGTGT10.7751937984496124No Hit
GATGTTCTATTGTCAATTGCGGCAAGGAGTTTAAGCTCTGTCTCTTATACGCATCTCCGAGCCCACGAGACGCGT10.7751937984496124No Hit
CCTTGCTCTCGGGATTGCTGGCTTTTGGAATAAGGTATTTGTCCAGAAGTCCCAAAACTTCGTAGCAGATTTCCC10.7751937984496124No Hit
CCAAGGCGATACAACGTGACCCTTCCATTGCTCTGCTGCGACATGCTGGGCAACTCTTCGCTCTAATACTTGGAA10.7751937984496124No Hit
GTGGTATTGTGTTGGCCTTCTGGTTCTGAGTATTGATTAACTGGTACAGTAGGTGGGTTTCGTATTTGATTTTGT10.7751937984496124No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT10.7751937984496124No Hit
ATAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCCAAAAAGACCAAGAATATAAAAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAATACAGAAAAAAACGATTAAGCCAAAAAAA10.7751937984496124No Hit
GGCATGAACCAATAGAGTGTCCTGAGTGCTTTAAAATCGTCAAAAACCGACGAAATTATGACACTCATGTCAATG10.7751937984496124No Hit
AAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAACTACAAAATGTAGATGAA10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAA10.7751937984496124No Hit
CAAACAATCTTACACACACACACATACTCTCAATTAAAAACAAAAAAAAAAAAAAAAAAAAAAAAATAAGATCCT10.7751937984496124No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCCCACCACCCCAGCAAAAGCAAAAACAAAGCAGA10.7751937984496124No Hit
ATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGTCTGTCTCTTATACACATCTCC10.7751937984496124No Hit
GTTCAGGGAGACGTGTGCTCTTCCGATCTGTTCAGGGAGACGTGTGCTCTTCCGATCCTGTCTCTTATACACATC10.7751937984496124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAAACCACCCCCAACAACCCGCCAATGGTAT10.7751937984496124No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT10.7751937984496124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAACCAAACAA10.7751937984496124No Hit
TTCAAAAGAAGTAATTTAACTTTTCTTTCAAAAGGAGTGAAAGTTACTATTATTGAATACTTAACTTTAAAGAGT10.7751937984496124No Hit
CTTTAGGAGCACATAGGTTCCTAGATTGGTGCTGTCCTTGCCGCGGGCGTGGCCCTGCAGTTCTTGCTTAACCAC10.7751937984496124No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAACCAACAAAAACGATTTAAAAAATAATCAATCC10.7751937984496124No Hit
GGGTTCCACCGCTTAATAACTACCTGGCCGGCGTCCTAAAATCCGAACAATAACTTCTTCTCCTCCGTGAGCTTC10.7751937984496124No Hit
GTGTCCGCTTTGTTGGGTCGTATTCCCTCAGCCGTCGGTTACCAGCCAACCTTGGCTCTGTCTCTTATACACATC10.7751937984496124No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers