FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length75
%GC15

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.51282051282051No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG25.128205128205128Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGATAATGAAATAAGA12.564102564102564No Hit
AAAAACTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAAAAAACTTAAAAAAAAAAAAAAAAC12.564102564102564No Hit
CTCACGCGGCATTGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCCCCACTGCCTGTCTCTTATACACATCTC12.564102564102564No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACACCACACAAAATAAAAAAAAAAAAAAAAA12.564102564102564No Hit
GTTGTTGGACTCGGTGGCACCGGAGGTCCAGACGATGCTTGCCGGCTCGGCGCCGACCAATTCCGCCACCTGGCG12.564102564102564No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTAAGAAAGTTTTAAACTCAACAAATTTAATATACGCTATTGGAGCT12.564102564102564No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAACAAAAAAAAAAAAAAAAA12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAA12.564102564102564No Hit
CAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAACCAAAACACAAACACCAAAAAAAAAAAA12.564102564102564No Hit
AAATATGAGAGAACATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAATTATAATAAAAAA12.564102564102564No Hit
ATAAAAAACGCTAATCTACCTAAAAAAAAAAAAAAATAAAAAACAAAACTTGCCCTCCAAATGGACATTGTTAAT12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAC12.564102564102564No Hit
CCAAACCACACACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGACGAGCCAAGAAACAAAACGAAAAAAAAAA12.564102564102564No Hit
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGGG12.564102564102564Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAA12.564102564102564No Hit
AAAAAAACTAGCTCTTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAAAAAAAAAAAA12.564102564102564No Hit
GCATATTACTGGCCGTCGTGGCCTTTGTTGGCCTCTCGCTGGGCGAGGAGAAGAAGGAAAAGGACAAGGAGCTGG12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGCAGGATCAAGAATTAATAG12.564102564102564No Hit
AGACACGAGAGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAAAGAAAAAAAAAAAAAAACAAA12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACGGACAAAAATAAAAAT12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTCGATGGCGGCGGAAAATAAATGATAACGT12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAA12.564102564102564No Hit
GGACAGATACGAAATCGTTCGGCTTGGATAAGATTGGTTGCCTTAAGCAAAGAAAGTTCATTAAACAGCCAATCA12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.564102564102564No Hit
AAACAACACAATAAGAACTGAAATATAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATAATAA12.564102564102564No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGTCAAAAAAAAATAA12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACAAAAATTAGACTCTTTGGCCTTAGTCGACCACCTCG12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAACAAAAAAATCAAAAA12.564102564102564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAAAAAAAA12.564102564102564No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers