FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.256410256410255No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA21.7094017094017095No Hit
AATTACCACAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAACCACACCCGAAACGAACAAAAAAAA10.8547008547008548No Hit
TATATATACCATGTGCTCCATGTACTTATGTATACTTTACTATATATCAAATGTCTCTTATACACATCTCCGAGC10.8547008547008548No Hit
GCGCTGGGTCCGATCTTTTTTTTTTTTTATAATTAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8547008547008548No Hit
TCTAAGGGCATCACAGACCTGTTATTGCCCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGT10.8547008547008548No Hit
CAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTG10.8547008547008548No Hit
GGGGTGATTTGGCGCAAACCCACCCGTGTGTGTAGATGGGCAGCATCCATGACTGCAGCCCATCGCCCAGCAAGG10.8547008547008548No Hit
TTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGCT10.8547008547008548No Hit
TCTAAATATACAAAAAGTCTATCCTAGTCGCACGTATAAGGCCACCAATGGTATGTCTGACTATTATACACATCT10.8547008547008548No Hit
CCGCCGAAGGATCTAGCTCCCTGGTGCGCAGCTGCAGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG10.8547008547008548No Hit
GATTCCAATGATGACGACGACGACGACGATGAGGACTGCGATGACTGTCTCTTATACACATCTCCGAGCCCACGA10.8547008547008548No Hit
CCTCTAAATAATCAAGTTCGGTCAACTTTTGCGAAACAACCGTAACACGCAAGGCGTCACAGTGATCACGTCCGG10.8547008547008548No Hit
AGCTGGACAAGTCCTACAACTACCAAGAGTACATGAAGGAGCATGAGTCTTATAGGCATCTCAGATCCCACGAGA10.8547008547008548No Hit
TGATAGCTTCGCACCATTGCCCGATCGGACAGATGCAAATACCAATTATCTGAATCAACGGTTCCTCTCGTACTA10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAGACAAAATATAAAAATAAGAA10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCGAACTAAAATAAAATA10.8547008547008548No Hit
CGAGCGCAACGAGAAGCTCAAAATCGAGATGATGTCCAGCTTAAAGGACCTGGGCAACATGATACTCAAACCGTT10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAATTGAA10.8547008547008548No Hit
CCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCC10.8547008547008548No Hit
TACCAAAACAAAAACAATAAACATTAGAATTACCAATAAAAAAACAAAAAAATACAATCCCTACTAACCATCACT10.8547008547008548No Hit
CCATAGATCTGGTACCCCAATCACACCCACCCACTCCCGAAACGAACCAACAATCCCCCTTACCCCATGCAACCA10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATATAT10.8547008547008548No Hit
CTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGCTGTCTCT10.8547008547008548No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCA10.8547008547008548No Hit
CTCCACTCCTTACTGCTTCTGCAGATACTCGTCGAACAGGTAGACGGCTGTCTCTTATACACATCTCCGAGCCCA10.8547008547008548No Hit
GCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGCTGTCTCTTATACACATC10.8547008547008548No Hit
CATACTAGAAGAAGCTCTTGAAAATCAAAATCATCCATACAACCGGCGTACTGAATACATTCAGAGCACTCACCA10.8547008547008548No Hit
ACTTTAATATACGCTATTTGGAGCTGGAATTACCGCGGCTGCTGGCTCCAGACTTGCCCTCCTGTCTCTTATAAA10.8547008547008548No Hit
GTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCAC10.8547008547008548No Hit
GCGATGGTCGAGGCGTCGTGCAGGGCGACCAGACGCTCCAGGTCATCGGCCAGTTCTGCGACATGTTCGGGATGA10.8547008547008548No Hit
ATATACAATACACTTCAAAACCTAAGGTCGCTAGCTCATTCCATATATATACTGTCTCTTATACACATCTCCGAG10.8547008547008548No Hit
TTGTCACCCTCCTCGGGGCCACGGGAACGGGGAAGACCGTGACAATGGCCAAGGTCTCGCTCTTATACACATCTC10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAACAAAAAGAAAGAATA10.8547008547008548No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG10.8547008547008548RNA PCR Primer, Index 11 (96% over 30bp)
GGACTATTACGTTGAACAAATTAGAGTGCTGAAAGCAGGCTTCACATGCATGAATATTCTGTGCATGGGATAATG10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCATGGGAGAAGGAAAGAAGACCACAGATCTGCTTACAGA10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAACTCCAGTCCACACTCCAGAAAACCAGAACCAACACC10.8547008547008548No Hit
CGCCGGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAGCGGAAGACCCCGGGGCGGTTGACAAAAGAACGGAG10.8547008547008548No Hit
AAAGGGATTTTACGCCAAAAACGTGTTCAATATCAAAAACAGAAACAGGCAGCCCCAACCACTCTTCAAGATTGA10.8547008547008548No Hit
GTAGTATCTAGGACTACCATGGTTCCAACGGGTAACGGGGAATCAGTGTTCGATTCCGGAGAGGGAGCCTGAGAA10.8547008547008548No Hit
AAAAAAAACTAATGAAACAAAAAAACAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8547008547008548No Hit
GAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGAGCTAGTGACG10.8547008547008548No Hit
GGTAGCGGTTTCTCAGGCTCGCTCTCCGGAATCGAGGCCGGAATCCCCCTGACCCCTTCCAACCATGCTAGGCCT10.8547008547008548No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGT10.8547008547008548No Hit
ATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCTAATAAAAGCACATGTCCCATAAGGTT10.8547008547008548No Hit
CTCCACCGGTAATACGATTACATACATAAAGGTATAGTACTAACCACAATTGTAAGATGTACTACCAGAAGGAAC10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAAACATAGGAAAAACAGACGCCA10.8547008547008548No Hit
ATATGAAAAAGATCATAAAACATATACAACAAAATAAAAAATGTAACATAAACACCAATAAGATCCAAATAGAAA10.8547008547008548No Hit
AGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTATAGAGCTAATACATGCAATTAAAA10.8547008547008548No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC10.8547008547008548No Hit
AACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8547008547008548No Hit
GTGTAAGACAGATTGATAGCTCTTTCTCGAATCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTCGTGGAGTGAT10.8547008547008548No Hit
GCCCTGTAATTGGAAAGAGTACACTTTAAATCCTTTAACAAGGAACAATTGGAGGGCAACACTGGTGCCAGCAGC10.8547008547008548No Hit
CTTCCGATCTGGGTTGAGCAAAAAAGCCAACCCATGCGGAAGCTACATGGAAATAAAAGATTTTCTGCTCCGGTC10.8547008547008548No Hit
GACTACAGGCGCCTGCCACCATGCCCAGCTAATTTTTTTTGTATTTTTAGTAGAGATGGGGCTGTCTCTTATACA10.8547008547008548No Hit
AATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAAC10.8547008547008548No Hit
TATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAACGTTATATTGGTTTAACTCTAGATAACA10.8547008547008548No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACACATGAAATAAAAATAAAAAAAC10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACGTCACAAACAAAAAAAAAAA10.8547008547008548No Hit
CCAGAATAACTATGGACGCACCACCACCACTCAATCCTCCTATCCGGATAGCGATGAGTACCCGTTGAGATCGAT10.8547008547008548No Hit
AAATATATCAGTTATCGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATT10.8547008547008548No Hit
CAACACCGCCACTACAATATAACCGACTATGCCGTCGAGACAACCACCAATTCGAAACGCCGATCATGCACTTCG10.8547008547008548No Hit
CTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATGCTGTCTCTTATACACATCTCCGAGCCCA10.8547008547008548No Hit
GAACCACCCGCAAGCAAACAAGAACACTGGTGGCAACACCACATCCAAACATCAGCAACCACAGTCCCCCTTAAT10.8547008547008548No Hit
CATCAAAGGAAGGCAGCAGGCGCGTAAATTACACACTCCCCGCTCGGGCAGCTAGTGACGAAAAATAACAATACA10.8547008547008548No Hit
GCGGTAAAACTGCGCTCTCGATCGACGGCGATCTTTGTTGCCGATCAAACAGGCAAGCGGAAAAAGTCATGGATT10.8547008547008548No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT10.8547008547008548No Hit
TCCATATACCGCAGCAAGCGTATCGCGCGATTGGAGAGCACAATGCTCTTGCTGGTCTGGGCCAGCTTCTGTTTG10.8547008547008548No Hit
AAACAGAACAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACCGACCAAGCTATATATTATACAA10.8547008547008548No Hit
AAAAATAACAATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACATTAAAAAAATAAACAACTCC10.8547008547008548No Hit
ACGTGTGTGCCGACCTCGATGCTTTAGGCGCTTAATGACGAGGCGCCGATGTGCTTCCAGAGTCGTCGATGCCAG10.8547008547008548No Hit
AAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCATGTTATCTAGACTTA10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAGAACAAACGAGACAGAA10.8547008547008548No Hit
ACCATCTAGTGCGCGATTCGCGCGCGTTTTGAATCAAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG10.8547008547008548No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATGACGATCTTGCGATCGCTTGGTTTTAGCC10.8547008547008548No Hit
CAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCC10.8547008547008548No Hit
GTACTATCAAGCACTACCAATAAAAAAAAAGTAAACACGCAATCACTTATCCAAAACTCAGAACAAACGAAAAGC10.8547008547008548No Hit
CAAAAACAAACCACCAAGAAAATAAGATATAAATAATAAATAATAATGCACACAAACGCCGAATTTAAAAAATAA10.8547008547008548No Hit
AGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCTCAAT10.8547008547008548No Hit
GGCCGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTT10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGACACACAGAACAGATAAAAAAAGACAACGAGGAA10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAATAA10.8547008547008548No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAGAACAAA10.8547008547008548No Hit
AAATAAAACAATAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAACCGATAAAAATAAAAAAAAATAAG10.8547008547008548No Hit
CTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCCTGTCTCTTATACA10.8547008547008548No Hit
TTATACGTATGGGGAAGCAAAACTGGGGAAAGGCAAACAAAGAGCTCGTTTGTATGTACGCCGCAGCGTTTTCTG10.8547008547008548No Hit
GTACTAACCACAATTGTATGTTGTACTACCCGTATGAAGAACAAGCTGTCTCTTATACAAATATCCGAGCCCACG10.8547008547008548No Hit
GGATTCTCTTGATGCTCAGGCCACGGTGCAAGTACAGGAACCACCCAAGTTCATTAAGGCACCCAAGGATACCAC10.8547008547008548No Hit
GGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAG10.8547008547008548No Hit
GTCAAGAGCATCATCACCCTGGATGGCAACAAGCTGACGCAGGAGCAGAAGCGCGACAAGCCCCCCACCCTCCTC10.8547008547008548No Hit
ATCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACATGCATG10.8547008547008548No Hit
AAAAAACCAACCAAAAACCTGCAAAGAAATTCGATATGATCGACGAAAAGCGACATTCCTCTCGAAACAACAATC10.8547008547008548No Hit
GTTTTATTTCAATCGTAGGATTGCAGAGCCTGAGCGGATTTCAACTCGAAAATGCCGCGTTGCTTGCGTGTTGAT10.8547008547008548No Hit
CCGGACGACAGCACGTCGTCGAGATGGCGCAGGCGCTTGATCGAGGCCGAGATCGTCTTCCAGTTGGTCAGCGTG10.8547008547008548No Hit
CTTCCGATCTGCGTAGGGAAGTAATCAATGCGATTTAATGACTCACACTTACTGGGAATTACCAAGTTCATGTGA10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCACGACAACCCCACTGAGATA10.8547008547008548No Hit
TCACTAAATAATTCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAAT10.8547008547008548No Hit
CCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGCGAAGCGACTGAGAGAGCCATAAAAGTAGCTACACCC10.8547008547008548No Hit
CCATAAACGATTCCAGCTAGCAATTGGGTGTAGATACTTTTATGGCTCTCTCAGTCGCCTGTCTCTTATACACAT10.8547008547008548No Hit
AGTGGGGTCCCATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAACAATAATGAGATTGAGCAATA10.8547008547008548No Hit
ACATTCATAATACTAAATATAATAAATAAAATAAAAAAATCAAAAAAAAATGACATATCCTTATATAATGCTAAA10.8547008547008548No Hit
GTACATGCCCTAAACCAAGCACAAACAGCACAGACCACCCTTCCCACGACACTCCAGGAAGTCCATGATGTCCTA10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGCAGGGGCAAGGGGGAA10.8547008547008548No Hit
GGTTTATATCCCAATAACTTGCACATATGTTAGACTCCTTGGTCCGTGTTGCAAGACCTGTCTCTTATACACATC10.8547008547008548No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers