FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4423568
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG89870.20316179156735018TruSeq Adapter, Index 11 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68630.1551462529794953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA27250.027.7323341
TTCGGAA28750.026.760992
TCGTATG27450.025.26123240
GATCTTT159750.024.5754911
ATCTTTT164950.023.57069412
CGATCTG243750.023.424165
GTATGCC30950.023.29627442
TCTTTTT174250.022.11470813
CCGATCT734850.022.0606024
GCCGTCT31500.022.01709246
CGATCTA122400.021.9566785
GATCTTA16500.021.9541176
TTCCGAT754400.021.7004992
CGTATGC33100.021.67884441
CCGTCTT32550.021.62487847
CTTTTTT181050.021.30316414
CGATCTT221550.021.2243675
TCCGATC774850.021.1132223
CTTCCGA783000.020.9995481
ATCTGTG21750.020.9375217
Produced by FastQC (version 0.11.2)