FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3031
Sequences flagged as poor quality0
Sequence length75
%GC21

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2347.720224348399868No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA40.13196964698119432No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA40.13196964698119432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT351.7723314E-549.2857172
TCCGATC351.7723314E-549.2857173
CCGATCT351.7723314E-549.2857174
CTTCCGA351.7723314E-549.2857171
GATCTTT305.353638E-446.06
TCTTTTT305.353638E-446.08
CGATCTT305.353638E-446.05
CTTTTTT305.353638E-446.09
ATCTTTT350.001144401439.4285747
Produced by FastQC (version 0.11.2)