FastQCFastQC Report
Thu 14 Mar 2024
H27HKDRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27HKDRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009129734
Sequences flagged as poor quality0
Sequence length10
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAACTGT26908046126.664605345976263No Hit
TGTGACGAAC26289420626.051576635041457No Hit
GACTCTCCCG25934460925.699828303741175No Hit
ATTGACAGTC15964003315.81957479017262No Hit
AGATCTCGGT188215221.8651241129715834Illumina Single End PCR Primer 1 (100% over 10bp)
TGTGACGAAG151407951.5003814167663798No Hit
GGGGGGGGGG19479990.19303751880132392No Hit
AGTTCTCGGT17798800.17637771834795624No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)