FastQCFastQC Report
Fri 5 Mar 2021
H273YDRXY_l02.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH273YDRXY_l02.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404433771
Sequences flagged as poor quality0
Sequence length9
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGG10938825927.04726134257468No Hit
AGATCTCGG7623854218.850686432909182Illumina Single End PCR Primer 1 (100% over 9bp)
CGGGCTCGG4897556812.10966331493618No Hit
GGGGCTCGG318100527.865330316344922No Hit
GGGGCGCGG285650547.0629744715359095No Hit
GGGGGGCGG257836436.375244811096648No Hit
CGGGCGCGG166687364.121499537188748No Hit
GGGTCTCGG146069483.611703336218181No Hit
GGATCTCGG116176902.8725815777634454No Hit
GGAGCTCGG89746042.21905405619552No Hit
GGGTCGCGG64191081.5871839743076253No Hit
GGGGGTCGG62893841.5551085124392345No Hit
GGGGGTGGG43111691.0659765106509862No Hit
AGAGCTCGG25268710.6247922852120081No Hit
CGGTCTCGG23964210.5925373131117678Illumina Paired End PCR Primer 2 (100% over 9bp)
GGGGCGGGG15705400.38833057786363745No Hit
AGGTCTCGG11087730.274154405369872No Hit
GGAGGTCGG10434730.2580083748743129No Hit
GGGTGTCGG8316490.20563292673202604No Hit
GGGTGGCGG7398780.18294169603358865No Hit
CGAGCTCGG6475000.16010037895673157No Hit
GGGTGGGGG5912150.14618339080294063No Hit
AGATCGCGG4191380.10363575696550821No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)