FastQCFastQC Report
Fri 5 Mar 2021
H273YDRXY_l02.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH273YDRXY_l02.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404433771
Sequences flagged as poor quality0
Sequence length6
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTT101005792.497461815571282No Hit
CCCCCC58362041.4430555553185989No Hit
AAAAAA43332151.0714275885729632No Hit
TTTTTC22403560.5539487947459264No Hit
TTTTTA22219690.5494024385021002No Hit
TATTTT21957940.5429304270438879No Hit
CTTTTT21765380.538169202492242No Hit
TCTTTT21748580.5377538069144082No Hit
TTTTCT21069880.5209723200884725No Hit
TTCTTT20976430.5186616821867727No Hit
TTTCTT20888260.5164815971809634No Hit
TTTTAT20760700.5133275579006976No Hit
TTTATT19934370.49289578243454846No Hit
TTATTT19500820.48217585667444174No Hit
ATTTTT18897800.46726562802293775No Hit
CCCCTC16538450.4089285115609201No Hit
CCCCCT16532440.4087799087381355No Hit
CCCTCC15998180.39556983484447933No Hit
TAAAAA15777020.390101448773426No Hit
CCTCCC15387100.3804603152193243No Hit
CTCCCC14788590.36566160049972685No Hit
TCCCCC14196910.35103176386326057No Hit
CAAAAA12656380.3129407311537295No Hit
CCCCCA12416920.3070198606139644No Hit
AAAAAT12257850.303086707365988No Hit
CCCCAC11644810.2879287249234189No Hit
AAAATA11353130.28071666646255417No Hit
CCCACC11248200.2781221749160013No Hit
CCACCC11233590.2777609291188495No Hit
CACCCC11214730.27729459813087665No Hit
AAATAA10545040.26073589190948154No Hit
AAAAAC9864330.24390470596976926No Hit
ACCCCC9863960.24389555737668606No Hit
CCTTTT9852580.24361417632455823No Hit
TTTTAA9660410.23886259488454042No Hit
AATAAA9642880.2384291493798128No Hit
GTTTTT9604490.23747992103260834No Hit
TAATTT9542730.23595284776552453No Hit
AATTTT9354120.23128929062652387No Hit
TTTTCC9098630.22497206347290916No Hit
TCTTTC9091740.22480170183414283No Hit
TCCTTT8934070.22090316488431921No Hit
CTCTTT8918650.22052189108609332No Hit
CTTTTC8904530.22017276099329497No Hit
ATAAAA8878400.2195266725142001No Hit
TTTAAT8783000.21716781905435886No Hit
TTTCCT8687180.21479858070507174No Hit
CCCCTT8670390.21438343238651056No Hit
CTTCTT8594470.21250623998953838No Hit
TTTATA8546710.21132532970398263No Hit
TATTTA8537250.21109142243217865No Hit
CCCTTC8530070.21091389027450927No Hit
TTCCTT8511930.21046536195415788No Hit
TTTCTC8505160.2102979674266618No Hit
TTAATT8499030.210146397492607No Hit
CTTTCT8497140.2100996654901007No Hit
TTCTTC8447920.20888265535075703No Hit
AAAACA8328790.2059370556372257No Hit
TCTCTT8291450.20501378951363583No Hit
TTAAAA8212850.20307033163162827No Hit
TATATT8200670.20276916983769885No Hit
AAACAA8188690.2024729532292198No Hit
TTCTCT8173520.20209786091280693No Hit
TCTTCT8172060.20206176105901896No Hit
TTATTA8010960.19807841417872096No Hit
TATTAT7991340.19759329148603663No Hit
TAAAAT7979300.19729559132192254No Hit
CCCTCT7941240.1963545225307112No Hit
TTATAT7939090.19630136178711938No Hit
CCTTCC7865460.1944807917635543No Hit
GCCCCC7778420.1923286470555398No Hit
CCTCTC7716830.1908057771960888No Hit
CCTCCT7611820.18820930757535578No Hit
TAAATA7587960.18761934695112292No Hit
AACAAA7584410.1875315699093783No Hit
CTCCCT7498290.18540217305443565No Hit
CCCTTT7361910.18203005109580722No Hit
TAATAA7357860.1819299110904366No Hit
CTTCCC7344160.18159116588708415No Hit
ACAAAA7295910.1803981398971749No Hit
CTTTTA7284220.18010909380759896No Hit
TCTTTA7211430.17830929356292552No Hit
TATAAA7199330.17801010984317628No Hit
CTCCTC7146320.1766993884395475No Hit
TTCCCC7137420.17647932768700467No Hit
TTTCTA7135520.17643234842522584No Hit
TCCCTC7129320.1762790476762634No Hit
CTCTCC7078080.17501209116387068No Hit
TCCCCT7055510.17445402698579293No Hit
AAAATT7052200.17437218416658878No Hit
TCCTCC7047130.17424682371542113No Hit
TTTTCA7036560.17398547066436745No Hit
TTTAAA7019820.1735715586421689No Hit
CCTTTC7013430.1734135599670286No Hit
TCTCCC6994030.17293387697833967No Hit
TTCTTA6946230.17175197765569383No Hit
TAAATT6867110.16979566229151521No Hit
TTCTAT6667800.1648675377309181No Hit
CTTTAT6632900.16400460286982316No Hit
CCTCTT6628510.16389605604918686No Hit
CATTTT6603930.16328829275733256No Hit
TCTTAT6603630.16328087497915697No Hit
AAATTT6545090.16183341919782462No Hit
ATATTT6503540.16080605692050381No Hit
TTAAAT6486120.16037533126777387No Hit
TTTTAC6452460.15954305655647139No Hit
TCTATT6417570.15868036895464896No Hit
TTTCAT6410210.1584983861300742No Hit
CCTTCT6401950.15829414997097263No Hit
CTTATT6396150.158150739592911No Hit
CTCCTT6325190.15639618779510872No Hit
TTTCCC6298370.15573303842620995No Hit
TCATTT6286820.15544745396644932No Hit
TCCTTC6283630.1553685782585154No Hit
CTATTT6259380.15476897452265428No Hit
TAATTA6258550.15474845200303514No Hit
CTTTCC6211130.15357594853274506No Hit
TCCCTT6179290.15278867500904122No Hit
ATTTTA6174510.15267048507677664No Hit
TTATAA6141010.15184216651383448No Hit
TAATAT6139940.15181570977167483No Hit
TTCATT6139760.15181125910476945No Hit
TGTTTT6138020.15176823599135097No Hit
CTTCCT6119910.15132044944881717No Hit
ATTATT6114400.1511842095896586No Hit
CTCTTC6113260.15115602203259135No Hit
TTGTTT6110250.15108159699156282No Hit
TCTTCC6106630.15099208913491055No Hit
TTTATC6076680.150251547613713No Hit
TTAATA6058240.14979560151518603No Hit
TTCCCT6043520.14943163586603653No Hit
TATTTC6025400.1489836020642302No Hit
AAATAT6004550.1484680664810259No Hit
TATTAA6004380.14846386307339304No Hit
CCAAAA5998640.14832193625096654No Hit
AAATTA5976700.14777944940705756No Hit
CTCTCT5964620.14748076020585332No Hit
CTTCTC5950840.1471400369283207No Hit
ATTTAT5936940.14679634653951784No Hit
TTCTCC5884610.1455024387664204No Hit
TCTCTC5869490.1451285827463701No Hit
TTTGTT5827530.14409108283887598No Hit
TTATTC5805610.14354909051351206No Hit
TTCCTC5805120.14353697480915856No Hit
TCCTCT5785210.14304468159757114No Hit
TATAAT5767420.14260480735175796No Hit
CCCCTA5703430.14102259526690217No Hit
TCTCCT5699680.14092987303970717No Hit
TATCTT5687650.14063242013486554No Hit
TATATA5687320.1406242605788724No Hit
TTTACT5669440.14018216099960654No Hit
CCCCAA5667960.14014556662727357No Hit
TTTTTG5642160.13950763770417185No Hit
CTAAAA5632060.1392579058389266No Hit
TATTCT5584290.13807674829409833No Hit
TCAAAA5558680.13744351729717447No Hit
TTTTGT5529540.13672300377705104No Hit
TACTTT5473670.13534156622148155No Hit
TTATCT5436570.13442423432043216No Hit
CGCCCC5387680.13321538373708164No Hit
CCCAAC5378400.13298592713218302No Hit
AATAAT5305530.13118414881332943No Hit
AATATT5303170.13112579562501472No Hit
CAAAAC5300240.13105334865816634No Hit
CCCTAC5255140.1299382093391009No Hit
CAACCC5244230.12966844947278153No Hit
TTACTT5209960.12882109194585534No Hit
CCCTCA5192730.1283950642193033No Hit
CCAACC5186640.1282444833223386No Hit
CCTCCA5151130.1273664656456199No Hit
AATATA5144490.12720228548866658No Hit
AATTAA5131970.12689271687947148No Hit
AATTTA5067380.1252956692382645No Hit
CTCCCA5058180.12506819070754605No Hit
CAAAAT5010520.12388975301471548No Hit
CCCCAT4937440.12208278225113896No Hit
TAAAAC4934660.12201404417337838No Hit
CCCGCC4912730.12147180458874192No Hit
CAAATA4867150.12034479682459555No Hit
AACCCC4849550.11990962050496025No Hit
AATTAT4845230.1198028044992316No Hit
CCGCCC4827800.11937183158722915No Hit
CAACAA4821630.11921927261608427No Hit
CAAACA4810240.11893764430468395No Hit
CCCCGC4789400.11842235598075217No Hit
CAATAA4769170.11792215047244411No Hit
CCCACA4751050.11747411667063778No Hit
ATAAAT4743970.11729905710569358No Hit
CCTACC4736990.11712647013347459No Hit
ATTTTC4736720.11711979413311654No Hit
CCCCCG4726870.1168762437496843No Hit
CACAAA4726630.11687030952714382No Hit
TCCCCA4721780.1167503887799716No Hit
CCCATC4684170.1158204466560237No Hit
CATAAA4679680.11570942724266219No Hit
CCTCAC4669760.11546414604432229No Hit
CCACCA4597740.1136833847636329No Hit
TACAAA4573310.11307933036086643No Hit
TAACAA4571340.11303062028417997No Hit
CCCAAA4554220.11260731240962565No Hit
AAAACC4551570.11254178870240784No Hit
CACCCA4534030.11210809593840768No Hit
CTCCAC4510990.11153841057452148No Hit
ATTAAA4488290.11097713202590097No Hit
CCACAC4481560.11081072653549497No Hit
TAAACA4477460.11070935023376177No Hit
TCCCAC4475060.11065000800835693No Hit
ACTTTT4439270.10976506707200769No Hit
CTACCC4438930.10975666025674201No Hit
ATTCTT4425960.1094359649802835No Hit
CCCACT4417660.10923073978409187No Hit
ATCTTT4412340.10909919785111122No Hit
TACCCC4407790.10898669488211458No Hit
CCTTTA4374000.10815120580026934No Hit
ATTTCT4370860.10807356638869803No Hit
ATAATA4357830.10775138755660442No Hit
CTCACC4312730.106636248237539No Hit
ATAATT4304500.10643275385625499No Hit
CCATCC4301620.10636154318576921No Hit
TCCACC4293460.10615977961939285No Hit
CCAAAC4292370.10613282835868817No Hit
TCACCC4221300.10437555670888819No Hit
CTTTAA4206940.1040204923935494No Hit
ATATAA4204000.10394779816742851No Hit
CACCCT4201550.10388721964566108No Hit
CCACTC4197850.10379573371482867No Hit
CCACCT4197770.10379375564064852No Hit
TTCTAA4175370.10323989487020362No Hit
TCTTAA4155660.10275254684406658No Hit
CACCAC4151590.10265191232015093No Hit
CACACC4136500.10227879807791819No Hit
TTTCAA4115180.10175164130890543No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)