FastQCFastQC Report
Fri 5 Mar 2021
H273YDRXY_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH273YDRXY_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419386342
Sequences flagged as poor quality0
Sequence length9
%GC75

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGG14162439733.76943472327003Illumina Single End PCR Primer 1 (100% over 9bp)
CGGGCTCGG6818423316.258095739321906No Hit
GGGGCTCGG4980640111.876018842788161No Hit
GGGTCTCGG363213788.660601064590702No Hit
GGATCTCGG283495736.759774976172209No Hit
GGGGCGCGG223052375.3185415847424045No Hit
GGGGGGGGG153035103.649024411958556No Hit
GGGGGGCGG122984682.9324913017792076No Hit
GGAGCTCGG105193122.5082628942646874No Hit
CGGTCTCGG103270082.462409231247688Illumina Paired End PCR Primer 2 (100% over 9bp)
GGGTCGCGG53814511.2831726885373869No Hit
AGAGCTCGG49780891.1869935907450224No Hit
GGGGGTCGG37571570.895870137802437No Hit
CGGGCGCGG28102850.6700945449482473No Hit
AGGTCTCGG14418990.3438116256060623No Hit
CGAGCTCGG10267190.24481460104392241No Hit
GGATCGCGG7547970.1799765334275001No Hit
GGGGGTGGG6200940.14785746170055294No Hit
GGGGCGGGG6041880.14406477738848253No Hit
GGAGCGCGG4725330.11267248183299207No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)