FastQCFastQC Report
Fri 5 Mar 2021
H273YDRXY_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH273YDRXY_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419386342
Sequences flagged as poor quality0
Sequence length6
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTT111734682.6642422227474447No Hit
CCCCCC56328101.34310763987636No Hit
AAAAAA49181721.1727067640176037No Hit
TTTTTC25317010.6036679658967053No Hit
TATTTT24347170.5805427492915352No Hit
TTTTTA24226210.5776585351937855No Hit
TCTTTT23846790.5686115071434539No Hit
ATTTTT23021160.5489248860660322No Hit
CTTTTT22903720.5461246041245663No Hit
TTTTCT22712140.5415565011413748No Hit
TTTTAT22171190.5286578932033986No Hit
TTTATT21756990.5187815582225136No Hit
TTCTTT21193300.5053407294794545No Hit
TTTCTT21154750.5044215293019724No Hit
TTATTT21104830.5032312187219488No Hit
TAAAAA16843790.40162943599150397No Hit
CCCCTC16322140.3891910242513334No Hit
CCCCCT16257470.3876490093232459No Hit
CCCTCC15804230.3768417904272142No Hit
CCTCCC15039350.358603714376564No Hit
AAAAAT14277490.3404376483009072No Hit
TCCCCC14193820.33844259048378833No Hit
CTCCCC14013330.3341389214816156No Hit
CAAAAA13446180.3206155912440277No Hit
AAAATA13371520.31883537113376No Hit
CCCCCA12616070.30082214742224483No Hit
AAATAA11913400.28406742916773386No Hit
AAAAAC11678050.27845565843438935No Hit
CCCCAC11625080.2771926225484949No Hit
AATTTT11182520.26664006144482405No Hit
ACCCCC11152600.2659266381164125No Hit
CACCCC11069000.2639332494046742No Hit
AATAAA10924200.26048058570300314No Hit
CCCACC10910230.26014747995775217No Hit
CCACCC10603230.2528272606455076No Hit
TTTTAA10536710.251241133646646No Hit
TAATTT10424920.24857557235376063No Hit
ATAAAA10330210.24631727277375193No Hit
CCTTTT10263380.2447237540224903No Hit
TTTTCC10169120.24247618440564286No Hit
TCTTTC10078440.24031397760683393No Hit
AAAACA9791520.23347255309520784No Hit
TTTATA9428250.22481061150055287No Hit
TCCTTT9367020.22335062117974264No Hit
CTTTTC9206280.2195178783385368No Hit
TATTTA9198990.21934405293532422No Hit
TTTAAT9187470.2190693658783957No Hit
TATATT9178330.21885142840440902No Hit
TTAATT9148760.21814635060290066No Hit
GTTTTT9027480.21525450630912538No Hit
TTTCTC8963520.2137294208784701No Hit
CTTTCT8938070.2131225818507938No Hit
CTCTTT8905910.21235574715020167No Hit
TTCTTC8764720.20898916159744657No Hit
CCCCTT8726030.20806662320920316No Hit
TCTTCT8698980.20742163320139786No Hit
TTAAAA8688480.20717126739430153No Hit
ACAAAA8670780.20674922217662492No Hit
AAACAA8664560.20660091024137356No Hit
AAAATT8648330.20621391623669044No Hit
TATTAT8592480.20488220858656384No Hit
TTATTA8531920.20343819398868263No Hit
TCTCTT8520870.20317471378216698No Hit
CTTCTT8500390.20268638123651628No Hit
TTTCCT8494100.2025364001958843No Hit
CCCTTC8465070.20184419835016942No Hit
TAAAAT8441990.2012938704618092No Hit
TAAATA8307950.19809777210150536No Hit
TTCCTT8200620.1955385566657295No Hit
TTATAT8198790.19549492148220696No Hit
AACAAA8180900.19506834583564No Hit
CCTTCC8046310.1918591330759169No Hit
ATATTT8044290.1918109674635041No Hit
CCCTCT8039390.19169413008685915No Hit
TCTTTA7985710.19041416470353248No Hit
TTCTCT7983100.1903519309171971No Hit
AAATTT7956950.1897284008357144No Hit
TATAAA7869550.18764440354616985No Hit
TAATAA7709930.1838383663910543No Hit
CTTTTA7692360.1834194209405131No Hit
TTTAAA7623920.1817875127654968No Hit
TAAATT7606340.18136832887132984No Hit
CCTCTC7585970.18088261920556298No Hit
CCCTTT7555690.1801606119066224No Hit
CCTCCT7467700.17806254644315528No Hit
TCCCTC7381440.1760057317269526No Hit
TTTTCA7304920.17418116110228501No Hit
ATTATT7272070.17339787379151225No Hit
TTTCTA7263260.17318780495717717No Hit
TCCTCC7256360.173023278855371No Hit
CTTCCC7244050.17272975475200383No Hit
TCTCCC7217440.17209525626373404No Hit
TCTTAT7200890.17170063206302508No Hit
ATTTTA7166370.17087752466674272No Hit
TTCTTA7145530.170380608150563No Hit
CCTTTC7093540.16914093973999755No Hit
CATTTT7085560.16895066172660436No Hit
CTCCCT7083650.1689051189940754No Hit
AAATAT7033610.1677119470905421No Hit
CTCTCC6990280.16667877085992466No Hit
ATTTAT6984960.16655191885099585No Hit
TCTATT6957190.16588976090213256No Hit
CTTTAT6942100.1655299494707913No Hit
TTCCCC6934410.16534658632254648No Hit
TTTTAC6884240.16415031465187774No Hit
CTCCTC6874440.16391663989858782No Hit
TCCCCT6850710.16335081317455016No Hit
AAATTA6847290.1632692654545245No Hit
TATTTC6840900.16311689997763448No Hit
CTTATT6741250.16074080924647757No Hit
TTTATC6738550.16067642946750993No Hit
TCTTCC6697550.1596988106017053No Hit
CCTTCT6647890.15851469955595263No Hit
TCCTTC6641430.15836066497368195No Hit
TCATTT6641210.15835541921391422No Hit
TTAAAT6639610.15831726823378525No Hit
TTCTAT6614190.15771114453698637No Hit
TGTTTT6613880.1577037527845864No Hit
TAATTA6601880.15741762043361918No Hit
CCTCTT6600600.157387099649516No Hit
TTAATA6590240.15714007205318098No Hit
CTTTCC6532890.1557725978591835No Hit
GCCCCC6520160.15546905912353245No Hit
CTATTT6497470.1549280305365786No Hit
TTTCCC6491890.15479497899337885No Hit
TATTAA6473620.15435934248903127No Hit
TTATAA6460540.154047458226477No Hit
TAATAT6459460.15402170631488996No Hit
TTTCAT6442260.15361158327850363No Hit
CCCCAA6373720.1519772906672292No Hit
TTATTC6332900.15100396378668907No Hit
TATATA6297750.15016583444198095No Hit
AATATT6290770.14999940079116833No Hit
TTTTTG6278470.14970611513142695No Hit
CCAAAA6257280.14920085308834402No Hit
TCCCTT6253350.14910714474340225No Hit
TCTCTC6199920.14783314045072074No Hit
TATAAT6179320.14734194658156036No Hit
TTCATT6146740.14656509724868436No Hit
CTCCTT6141650.1464437294431491No Hit
TTTTGT6101670.14549043182717666No Hit
CCCCTA6095400.1453409276737963No Hit
TATTCT6085600.1451072529205064No Hit
AATAAT6069020.14471191338892006No Hit
TTTACT6058860.14446965466510114No Hit
ATTTTC6024300.14364559349431555No Hit
CTCTTC6002120.14311672553227783No Hit
CTTCCT5985830.14272830086583982No Hit
TTCTCC5981020.14261360948182714No Hit
AAAACC5968590.1423172240549503No Hit
TCAAAA5932950.14146741097257765No Hit
CTCTCT5898330.14064191914003724No Hit
AATATA5884530.14031286693642495No Hit
TTGTTT5883880.14029736810074755No Hit
CTAAAA5875540.14009850611682534No Hit
CTTCTC5874660.1400775230777544No Hit
TCCTCT5836990.13917930593934313No Hit
TTCCTC5824700.13888625872322757No Hit
TTATCT5792330.13811441670649352No Hit
AATTAA5773100.13765588961406855No Hit
TATCTT5767020.1375109158895785No Hit
AATTTA5694390.13577909983534941No Hit
TTTGTT5660620.13497387571100253No Hit
TCTCCT5660020.13495956909345416No Hit
AATTAT5626460.1341593522852492No Hit
TACTTT5608770.13373754551119835No Hit
TTCCCT5573030.13288534799256768No Hit
ATAAAT5516590.13153957216851853No Hit
ACTTTT5443780.129803464129025No Hit
CCCTCA5369490.12803206643291212No Hit
CAAAAC5326360.1270036590748108No Hit
ATTTCT5310330.12662143394264375No Hit
CAAAAT5263150.125496456916091No Hit
AACCCC5260930.12544352243116205No Hit
CAAATA5257240.12535553673323965No Hit
ATAATT5250870.12520364814360121No Hit
ATAATA5227010.12463472165242806No Hit
TTACTT5220300.12447472597951223No Hit
CAACCC5210590.12424319721885459No Hit
TAAAAC5203880.12408320154593876No Hit
CCCTAC5182870.12358223148812032No Hit
ATTAAA5176820.12343797309450769No Hit
CCCCAT5135860.12246130800320626No Hit
CAAACA5130670.12233755576141295No Hit
CATAAA5129070.12229940478128398No Hit
CCTCCA5123520.12216706856896165No Hit
CCAACC5119180.12206358403536184No Hit
CCCAAC5117920.12203354013851028No Hit
CACAAA5052460.12047268816398414No Hit
CCCAAA4980430.11875517872730343No Hit
CAATAA4951490.11806512287422083No Hit
CCCACA4942780.1178574384761438No Hit
ATATAA4896960.11676488978270064No Hit
CTCCCA4866440.11603715983674069No Hit
ATCTTT4834320.1152712789106518No Hit
ATTCTT4832070.11521762909484544No Hit
CGCCCC4828510.1151327431640585No Hit
CAACAA4818790.11490097595977505No Hit
TAAACA4815920.11483254263916873No Hit
CCTACC4805540.11458503815558209No Hit
TACAAA4790640.11422975715313115No Hit
TCTTAA4717430.11248411136860534No Hit
TCCCCA4717370.1124826807068505No Hit
CCTTTA4683870.11168389456040036No Hit
AAAATC4639550.1106271124108281No Hit
TCCCAC4621860.11020530563677726No Hit
CCTCAC4608400.10988436051644238No Hit
ATTTAA4594980.10956436917061071No Hit
CCCATC4572410.10902620190716655No Hit
TAACAA4558780.10870120324519295No Hit
CTTTAA4542410.10831087102974851No Hit
CCGCCC4522460.1078351759962655No Hit
ATATAT4511050.10756311181922085No Hit
ATTAAT4472620.10664677296524835No Hit
CCCGCC4464280.10644791098132615No Hit
ATATTA4463990.10644099611617777No Hit
CACCCA4441310.10590020597284974No Hit
CCACCA4437500.10580935895141763No Hit
AAACCC4417250.10532651060916047No Hit
TACCCC4412540.10521420366140584No Hit
CCCACT4411240.10518320599005104No Hit
CCCCGC4406640.1050735219221803No Hit
ATTATA4375780.10433768489294293No Hit
CTCCAC4342910.10355392069491857No Hit
TTCTAA4342060.10353365298672508No Hit
TCCACC4337670.10342897623499624No Hit
CAAACC4328360.10320698521937083No Hit
TCTAAA4322440.10306582659289366No Hit
CCCTTA4320170.1030116998898357No Hit
CTACCC4315170.1028924780769327No Hit
TTTCAA4303010.10260253062795259No Hit
TCCTTA4283420.10213541956499861No Hit
CACACC4263870.10166926227654785No Hit
AAACAC4259370.10156196264493515No Hit
CCATCC4251300.10136953863890971No Hit
TCACCC4224920.10074052435403344No Hit
TCTATA4205510.10027770527634397No Hit
CTCACC4202200.10019878043620219No Hit
CCCCCG4199300.10012963178471844No Hit
CTTAAA4195650.10004259986129925No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)