FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n02_CITE-seq_test_TCells_PI1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n02_CITE-seq_test_TCells_PI1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912217
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26242813.723756247329671No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT404442.115031923678118No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT306451.602590082610917No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC237181.2403404006972012No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG196401.0270800855760618No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT194381.0165164309280799Clontech SMART CDS Primer II A (100% over 26bp)
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC111280.5819423213997157No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA108570.5677702896690072No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC107030.559716810382922No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA84260.44064036665294787No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC81940.4285078524037806No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT78270.40931546994927875No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG69040.36104688955280706No Hit
GCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCAACAC68300.3571770358698829No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC62250.3255383672459768No Hit
CAAAGACAGGGTGCCTGGAGACTGCGTCAACACAATCTTTCCGGTGGTAT53880.28176718437290327No Hit
GCCTGGCCAGGTTTCTGCTGGTACCAGGATAAGTAGCTGCTGCCAACACT52980.277060605569347No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG52890.27658994768899137No Hit
GCCCTGGTGGATACACCATACATGAGGAGCCGGGGAGCCTGGCCAGGTTT51540.2695300794836569No Hit
GCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAG46490.24312094286370217No Hit
GGTTTCTGCTGGTACCAGGATAAGTAGCTGCTGCCAACACTCTGACTGGC40940.2140970402417717No Hit
CTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGA40080.20959964271837347No Hit
CTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCAACACA39540.20677569543623972No Hit
CACCATACATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGCTGGTACCAG38450.20107550555193265No Hit
AGTAGCTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTT36500.19087791814422736No Hit
GTGAAGTCTATCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGT36240.18951823982319999No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT36180.18920446790296291No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA35710.1867465878611057No Hit
GTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGCGAGCTACGA35590.18611904402063154No Hit
GACAAAGACAGGGTGCCTGGAGACTGCGTCAACACAATCTTTCCGGTGGT35470.1854915001801574No Hit
GCTGGTACCAGGATAAGTAGCTGCTGCCAACACTCTGACTGGCCCTGCAG34690.18141246521707527No Hit
GTAGCTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTT34600.18094180733671963No Hit
GTCTATCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCCC34400.17989590093592933No Hit
CCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCCCTGGTGGATACAC33450.1749278455321755No Hit
CCCTGGTGGATACACCATACATGAGGAGCCGGGGAGCCTGGCCAGGTTTC33410.17471866425201743No Hit
GCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCC33260.1739342344514247No Hit
CCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCCCTGGTGG31900.16682207092605075No Hit
CTGCTGGTACCAGGATAAGTAGCTGCTGCCAACACTCTGACTGGCCCTGC31790.1662468224056161No Hit
GTTTCTGCTGGTACCAGGATAAGTAGCTGCTGCCAACACTCTGACTGGCC31520.1648348487645492No Hit
GGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACT31130.16279533128300816No Hit
CTATCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCCCTG30990.16206319680245496No Hit
CGTCAACACAATCTTTCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGA30590.15997138400087438No Hit
GGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCA30450.15923924952032117No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT29970.1567290741584245No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGACGTTATT29110.15223167663502624No Hit
CAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGAC29090.15212708599494723No Hit
GTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCAA28540.14925084339277395No Hit
GTGAGAGTGAAGTCTATCCCAGACCCACTGCCACTGAACCTGTCTGGGAT28460.14883248083245781No Hit
CTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTATCCCAGACCCA28290.14794346039178607No Hit
GCTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC28180.14736821187135143No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC27990.14637460079060066No Hit
CAATCTTTCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGC27940.14611312419040307No Hit
GCCAGGTTTCTGCTGGTACCAGGATAAGTAGCTGCTGCCAACACTCTGAC27790.14532869438981036No Hit
GGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGC27520.14391672074874345No Hit
ATACACCATACATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGCTGGTAC27520.14391672074874345No Hit
CCTGTCTGGGATGCCGGTGGCCCTGGTGGATACACCATACATGAGGAGCC26870.14051752494617503No Hit
CTGGAGACTGCGTCAACACAATCTTTCCGGTGGTATCTGGGAGCCAGAGT26590.13905325598506862No Hit
GGAGCCGGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGATAAGTAGCTG26150.13675226190333No Hit
CTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCC25990.13591553678269777No Hit
CCTGGAGACTGCGTCAACACAATCTTTCCGGTGGTATCTGGGAGCCAGAG25670.13424208654143333No Hit
GACTGCGTCAACACAATCTTTCCGGTGGTATCTGGGAGCCAGAGTAGCAG25620.13398060994123576No Hit
CCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG25570.13371913334103816No Hit
GTCTGGGATGCCGGTGGCCCTGGTGGATACACCATACATGAGGAGCCGGG25020.1308428907388649No Hit
CCATACATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGA24980.13063370945870684No Hit
CACAATCTTTCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAA24930.13037223285850924No Hit
GCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTATCCC24750.129430917097798No Hit
GGTACCAGGATAAGTAGCTGCTGCCAACACTCTGACTGGCCCTGCAGGAG24700.12916944049760043No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG24410.12765287621645452No Hit
CTGATGGTGAGAGTGAAGTCTATCCCAGACCCACTGCCACTGAACCTGTC24350.12733910429621742No Hit
CCAGGTTTCTGCTGGTACCAGGATAAGTAGCTGCTGCCAACACTCTGACT24090.12597942597519007No Hit
CTGGTACCAGGATAAGTAGCTGCTGCCAACACTCTGACTGGCCCTGCAGG23620.12352154593333288No Hit
GGTGGATACACCATACATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGCT23590.12336465997321434No Hit
GTCCCTTGGCCGAACGTCCAAGGCGAGCTACGATACTGCTGACAGTAATA23500.12289400209285872No Hit
CCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCCCTGGTGGA23340.12205727697222647No Hit
CTGCGTCAACACAATCTTTCCGGTGGTATCTGGGAGCCAGAGTAGCAGGA23310.12190039101210794No Hit
GACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGG23270.12169120973194987No Hit
CTTTCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGCTGCG23040.12048841737104105No Hit
CCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTG23040.12048841737104105No Hit
GCCACTGAACCTGTCTGGGATGCCGGTGGCCCTGGTGGATACACCATACA22940.11996546417064591No Hit
GGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTATCCCAGACCCACTGCC22860.1195471016103298No Hit
CTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGT22700.11871037648969755No Hit
GAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTATCCCAGA22550.11792594668910483No Hit
CTCCAGTCTGCTGATGGTGAGAGTGAAGTCTATCCCAGACCCACTGCCAC22530.11782135604902581No Hit
CCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCT22450.11740299348870971No Hit
CCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCAACACAATCTTT22280.11651397304803797No Hit
CTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCAACACAAT22260.11640938240795892No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA21950.11478822748673398No Hit
CTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGG21770.11384691172602271No Hit
GCCAGAGTAGCAGGAGGAAGAGAAGCTGCGCTGGGGTTTCCATGGTTCCC21770.11384691172602271No Hit
GCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTG21690.1134285491657066No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21690.1134285491657066Clontech SMART CDS Primer II A (100% over 25bp)
GGATGCCGGTGGCCCTGGTGGATACACCATACATGAGGAGCCGGGGAGCC21560.11274871000519293No Hit
GGATACACCATACATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGCTGGT21410.1119642802046002No Hit
GGTGCCTGGAGACTGCGTCAACACAATCTTTCCGGTGGTATCTGGGAGCC21400.1119119848845607No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATCGAAGTTATT21150.11060460188357284No Hit
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC21130.11050001124349382No Hit
GAAGTCTATCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGG20950.10955869548278255No Hit
CACTGAACCTGTCTGGGATGCCGGTGGCCCTGGTGGATACACCATACATG20890.10924492356254546No Hit
CCTGGCCAGGTTTCTGCTGGTACCAGGATAAGTAGCTGCTGCCAACACTC20780.10866967504211081No Hit
GGATAAGTAGCTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGG20770.1086173797220713No Hit
GGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCAACA20420.1067870435206883No Hit
CCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA20350.1064209762804117No Hit
GGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTC20320.10626409032029314No Hit
GGTGGCCCTGGTGGATACACCATACATGAGGAGCCGGGGAGCCTGGCCAG20300.10615949968021411No Hit
ACCAGGATAAGTAGCTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGG20250.10589802308001654No Hit
AGTCTATCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCC19970.10443375411891014No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG19960.10438145879887062No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG19950.10432916347883112No Hit
GCTGACAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTG19900.10406768687863353No Hit
GAGTGAAGTCTATCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCG19890.10401539155859402No Hit
CCAGAGTAGCAGGAGGAAGAGAAGCTGCGCTGGGGTTTCCATGGTTCCCT19660.10281259919768519No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA19600.1024988272774481No Hit
CCGGTGGCCCTGGTGGATACACCATACATGAGGAGCCGGGGAGCCTGGCC19440.10166210215681588No Hit
GACAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGA19440.10166210215681588No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT19430.10160980683677637No Hit
ACACAATCTTTCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGA19420.10155751151673686No Hit
CAACACAATCTTTCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGA19290.10087767235622316No Hit
CCAGGATAAGTAGCTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGG19190.10035471915582804No Hit
GATACACCATACATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGCTGGTA19160.10019783319570948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)