FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n02_CITE-seq_test_TCells_GD3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n02_CITE-seq_test_TCells_GD3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9701512
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4177004.305514439398725Clontech SMART CDS Primer II A (100% over 26bp)
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT2997963.0901987236628683No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC2571652.650772374450498No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC2047852.110856534527814No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG1953092.013181038172194No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1631251.6814389344671221No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC962350.9919587792088491No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT705460.7271650027335945No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT696010.7174242530442677No Hit
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC518190.5341332361388617No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT446240.45996953876880226Clontech SMART CDS Primer II A (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT363350.3745292486367074Clontech SMART CDS Primer II A (100% over 24bp)
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA324340.33431902161230126No Hit
CTGCTGGCCGCGGCCGTCGGGGGCGCCCCGGGGAACAAGCGGCCCGGGGA196100.2021334406430668No Hit
TAGCTGCTGGCCGCCACCTTGGGGTAGAGGAACTGTGAGCTCGTCGGCGT186980.19273284411749425No Hit
CTGCTGGCCGCCACCTTGGGGTAGAGGAACTGTGAGCTCGTCGGCGTGGT178060.18353840102450011No Hit
TAGCTGCTGGCCGCGGCCGTCGGGGGCGCCCCGGGGAACAAGCGGCCCGG177310.1827653256523313No Hit
TGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC174410.1797761008799453No Hit
CTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCGGGC166030.1711382720549127No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT163420.16844796975976528Clontech SMART CDS Primer II A (100% over 23bp)
CTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCAC157870.16272721200571622No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT135200.13935972042296088No Hit
GTCCCAGGTCACCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCC129610.13359773198239616No Hit
GCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA120380.12408375106890555No Hit
TGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCGG114250.1177651483603793No Hit
GCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCGGG102930.106096864076445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)