FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n02_CITE-seq_test_BCells_PI2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n02_CITE-seq_test_BCells_PI2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44030270
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC623100314.151634773077703No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC25680555.832476157879568No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC23923735.433473380926349No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT11672302.6509717065100897No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC11486852.608852955023896No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC9515512.16112915046853No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT4446421.009855265479862No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC4430741.0062940790506167No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG2318720.5266195278838853No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG2279340.5176756808441102No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT2091040.47490964738576436No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT1554840.3531297900285417No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGA1325750.30109967529156645No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA1267660.2879064788837316No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTA1200780.27271692860389No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG1177530.26743647041001567No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG1143760.2597667468312141No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAG1071690.24339846201261087No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC1006140.22851097665310705No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT945440.21472500622866952No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG933540.21202232009933167No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC900300.20447296825570224No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTTA882470.20042348139132465No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATG774280.17585174926249603No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA736020.16716227268195266No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG733090.16649682139128377No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT732740.16641733062277383No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACT697860.158495507749555No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACC680850.15463225639997213No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGA665080.15105062948739584No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGGA653010.14830933355621032No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAG638300.1449684501139784No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCG636420.1445414711288393No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC610980.13876362784057422No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTC610660.138690950566508No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG605760.13757807980736889No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG591000.13422584054106412No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCA575120.1306192308155276No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT545960.1239965142162426No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA535220.12155728320539484No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTG530260.12043078545736831No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTTACA521420.11842307576128877No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCT520400.11819141695020266No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG518460.11775081097617617No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA512380.11636994276891784No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGA498580.11323573532481176No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGCA494400.11228638843232167No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG481320.10931570485486462No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCT445970.10128713723536105No Hit
CTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCA440460.10003572542253318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)