FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n02_CITE-seq_test_BCells_PI1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n02_CITE-seq_test_BCells_PI1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51155261
Sequences flagged as poor quality0
Sequence length90
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC826377116.154293494856766No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC49924239.759353979251518No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC34326646.710285380031586No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC20211683.9510462081309683No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT14529452.84026505113521No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC12543192.451984361882153No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG11237492.1967417974858927No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT6018561.1765280603299042No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA5403731.0563390537680963No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC5303621.0367692191033882No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTC3424590.669450205717844No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG3036560.5935968149981681No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG3015720.5895229427135559No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACC2761520.5398310840404079No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAG2343480.4581112390375645No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTCA1701670.3326480926370408No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGA1458120.28503813126864896No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG1306670.25543218321181077No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGA1296880.2535184015579551No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCG1285430.25128011760119845No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG1280380.2502929268604455No Hit
CTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCA996580.19481476206328027No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTCACT880560.1721347878569127No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTAGAATG825750.1614203473617308No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATG779420.1523636053777538No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCT770570.15063357803999866No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACC705120.13783919507321055No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC679360.13280354487879556No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAC677930.13252400373834472No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAG606480.11855672088155313No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGA589380.11521395619504317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)