FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n02_CITE-seq_test_BCells_C1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n02_CITE-seq_test_BCells_C1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57466282
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC845944114.720703524894823No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC45313977.885314383136881No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC36506906.352751340342498No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC18751393.263024742056568No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT13223162.3010293235953565No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC9703361.6885310241577836No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT5727840.9967305697626305No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC4266260.7423936004768849No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG3956320.6884593647453997No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG2346530.4083316195747621No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC2307600.40155721227971564No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT2228900.3878622250174459No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA1767460.30756470376837675No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG1414510.24614607919127254No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG1259530.21917722117467073No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG1185110.2062270184801585No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAG1154440.20088997579484957No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA1147270.19964228762876984No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGA1093810.1903394411352382No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCA1067960.1858411511640861No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA1051730.18301688631952906No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT1050660.1828306901775897No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTA1033370.17982196934195255No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACC945770.16457824781495348No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC797560.13878747193006152No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA753270.1310803437744589No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG751840.13083150220158665No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA734180.1277583957841574No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC731010.12720676796177627No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG726350.1263958576613674No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT722670.12575548214516472No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTC717420.1248419029440603No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG699140.12166090717335776No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCG695170.12097006728223691No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC693510.12068120223960199No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG683500.11893931122949628No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT681210.11854081668272883No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA658790.11463939845629825No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGA627980.10927799365895988No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG620320.10794503810077707No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATG619340.10777450331657093No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCACT603490.10501636420466526No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG602980.10492761651084369No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACT578960.10074777414693367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)