FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n01_CITE-seq_test_TCells_PI2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n01_CITE-seq_test_TCells_PI2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8766461
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGTCGCAAACTCTGGCGCTAT661970.7551165744078483No Hit
GTGTTAGTCGCAAACTGTCATGGGTC621220.7086325941562964No Hit
TTCTACAGTGATGTGGCTTACAATAT396910.4527596712059747No Hit
GTGTTAGTCGCAAACTTCTCTTACTT354130.40396004727563384No Hit
GTGTTAGTCGCAAACTATAAGTCGCA346460.3952107925877957No Hit
TTCTCTCACTAGTACCTCGCTAAATT334330.38137396607365276No Hit
GTGTTAGTCGCAAACTACGACTACAT326580.37253345449206926No Hit
GTGTTAGTCGCAAACTATAACATGTA311460.3552859015741928No Hit
GTGTTAGTCGCAAACTTTACGCGCGT308120.3514759262603233No Hit
CTTCTCTCACTAGTACCGTTGCCTAC304650.34751765849411753No Hit
GTGTTAGTCGCAAACTTGCTCATCAT294830.33631587478687236No Hit
TTCTACAGTGATGTGGGGTCTAAGCA293800.33514094227990066No Hit
GTGTTAGTCGCAAACTTTATTGTACA288840.3294830148676872No Hit
GTGTTAGTCGCAAACTCCCATGTGGC287150.3275552129873161No Hit
GTGTTAGTCGCAAACTTTTGTTGGGG284370.3243840359296642No Hit
GTGTTAGTCGCAAACTCCCTCGCATT282860.3226615620602202No Hit
TTCTACAGTGATGTGGCTTGATGTGG279800.3191709858744595No Hit
TTCTACAGTGATGTGGGGGCGTGCCG279530.3188629938580689No Hit
TTCTACAGTGATGTGGTCAAAGGGAC277870.3169694133128522No Hit
GTGTTAGTCGCAAACTGGAGTCCTGG277110.31610247282227116No Hit
TAAGTGCGTACCGGCTATTCGAGCAT276250.31512146121450835No Hit
GTGTTAGTCGCAAACTAGTACCCTCG272420.31075253742644837No Hit
GTGTTAGTCGCAAACTCTGTCCACCC261020.297748430067732No Hit
CTTCTCTCACTAGTACGCCCCAATGG256940.29309432848671774No Hit
GTGTTAGTCGCAAACTGTGGCATCTC249750.2848926151613519No Hit
CTTCTCTCACTAGTACACTGGATCCC248960.28399145333561626No Hit
TTCTACAGTGATGTGGCGGGAACCAC246960.28171003099198183No Hit
GTGTTAGTCGCAAACTAAAAGCGGAC235750.26892265875591076No Hit
CTTCTCTCACTAGTACTGAAAGAGCT231720.2643255927334873No Hit
TAAGTGCGTACCGGCTTTGGGAGCGC223980.255496488263622No Hit
TTCTACAGTGATGTGGCCTGTACGGT223260.2546751762199136No Hit
GTGTTAGTCGCAAACTGGCCTGGGAC221860.2530781805793695No Hit
GTGTTAGTCGCAAACTTTGTGCCACG197300.22506231419953843No Hit
TAAGTGCGTACCGGCTGTGCTCCACC178970.2041530784201287No Hit
TTCTACAGTGATGTGGACTTATCTGA178880.20405041441466515No Hit
GTGTTAGTCGCAAACTCCTCCCGCCA176690.2015522569483854No Hit
TTCTACAGTGATGTGGCATCTACAAC171400.19551789484947235No Hit
GGGAATGCACCACCAGCCTTGACTTA158550.180859756291621No Hit
GTGTTAGTCGCAAACTTTCTGGTCAT148330.16920168811564895No Hit
TAAGTGCGTACCGGCTTTTCTAATTG115090.1312844487644444No Hit
TAAGTGCGTACCGGCTAAAGATTTGT111190.12683567519435723No Hit
CGATGTACAACTTGACTACATTCTCG110770.12635657650219398No Hit
CTTCTCTCACTAGTACCAGACATTCC104600.1193183885720817No Hit
GTGTTAGTCGCAAACTCTCGCTTATG103950.11857692631040051No Hit
TAAGTGCGTACCGGCTTTTGCATACC98940.11286196333959622No Hit
CTTCTCTCACTAGTACTCTCCAGGCA97830.1115957739388791No Hit
CTTCTCTCACTAGTACTCCCCCTACC94860.10820786175858195No Hit
GTGTTAGTCGCAAACTTGGGGTAACG93930.10714700036879192No Hit
GTGTTAGTCGCAAACTGACCGCACTA93100.10620021009618362No Hit
CTTCTCTCACTAGTACCGGATATAGG92660.10569829718058404No Hit
TTCTACAGTGATGTGGGGCCAGAAAA91100.10391878775254917No Hit
CTTCTCTCACTAGCCCCCCCTCTCTG89830.10247008456434131No Hit
CTTCTCTGGCTCACTCTCCTCACTCT88120.10051946846053383No Hit
TAAGTGCGTACCGGCTGCTGAGTAAC87930.10030273333788858No Hit
CTTCTCTCACTAGTACCCTGGAGGTA87690.10002896265665245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)