FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n01_CITE-seq_test_TCells_PI1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n01_CITE-seq_test_TCells_PI1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912217
Sequences flagged as poor quality0
Sequence length26
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACACTCGGCGGTTTGCAACCTCA554172.8980497506297667No Hit
CTCACACTCGGCGGTTCATGCAAACG389132.034967788697622No Hit
CTCACACTCGGCGGTTCCCGAGCGCC312441.6339149793145862No Hit
CTCACACTCGGCGGTTGATCTGTACC301971.5791617792332144No Hit
CTCACACTCGGCGGTTTTTCTGTTCT187040.9781316660190763No Hit
CTCACACTCGGCGGTTGTGCCATCAC119620.6255566183126706No Hit
CTCACACTCGGCGGTTTATCGCATTG88500.46281358234970194No Hit
CTCACACTCGGCGGTTTCACCAAGCG88260.46155849466875365No Hit
GCATGGGAGAAGAGCTGCTCAGTTAG84220.44043118537278986No Hit
CTCACACTCGGCGGTTCTCTCAGCCT82850.4332667265273763No Hit
TCGTAGAGTTCCCGAGTCCCATCCTG79790.417264358595285No Hit
CTCACACTCGGCGGTTCGCCCACGTC76400.39953624510188956No Hit
GCATGGGGAGCTGCTCAGTTAGGACC76200.39849033870109934No Hit
CTCACACTCGGCGGTTCCCCGCGCGC74930.391848833056081No Hit
GCATGGGGCTTTCTGAGAGTCATGGA74790.3911166985755278No Hit
CTCACACTCGGCGGTTCTCGGGTTGT72360.37840893580592583No Hit
CTCACACTCGGCGGTTGTTGGTCCAC69170.36172672871332073No Hit
CTCACACTCGGCGGTTAATTTTTACG68920.36041934571233286No Hit
CTCACACTCGGCGGTTGTAGTAGGAC68190.3566017873494483No Hit
CTCACACTCGGCGGTTTGTCATAGGC68100.3561311294690927No Hit
CTCACACTCGGCGGTTCGCCATGGGG67940.35529440434846044No Hit
CTCACACTCGGCGGTCCACCTCACAT67870.35492833710818383No Hit
CTCACACTCGGCGGTTAAACGACCAG67590.3534640681470775No Hit
CTCACACTCGGCGGTTGGAGGATTAG66270.3465610859018615No Hit
CTCACACTCGGCGGTTAACCTGCCTA65640.34326648073937216No Hit
CTCACACTCGGCGGTTTCGAGCACTG65530.3426912322189375No Hit
TCACACTCGGCGGTTATCGTACCCGT64800.33887367385605294No Hit
CTCACACTCGGCGGTTCACTCAGCTG64700.3383507206556578No Hit
CTCACACTCGGCGGTTCATCGCATGG64480.3372002236147885No Hit
CTCACACTCGGCGGTTGGCTGCATCG64260.3360497265739192No Hit
CTCACACTCGGCGGTTCGCATAGCGG64070.3350561154931684No Hit
CTCACACTCGGCGGTTTGAAATAGCC63630.33275512141142977No Hit
CTCACACTCGGCGGTTCGCGCACGCG63410.3316046243705605No Hit
CTCACACTCGGCGGTTATACTTAGCT63370.33139544309040236No Hit
CTCACACTCGGCGGTTCGGTCTACGC63340.33123855713028383No Hit
CTCACACTCGGCGGTTCATACTGCGC63300.3310293758501258No Hit
CTCACACTCGGCGGTTAGGTTCATCG62330.3259567298062929No Hit
CTCACACTCGGCGGTTCACATTGGTG61940.32391721232475185No Hit
CTCACACTCGGCGGTTTCTCCCGCCA61690.32260982932376403No Hit
CTACACTCGGCGGTTTAGGCATTTGT61540.3218253995231713No Hit
CTCACACTCGGCGGTTCGAGACCCAG61160.31983817736166975No Hit
CTCACACTCGGCGGTTTTCGTCAATC61140.3197335867215907No Hit
CTCACACTCGGCGGTAAACCCCGATC61020.31910604288111655No Hit
CTCACACTCGGCGGTTCCCCGCTCCG61010.319053747561077No Hit
CTCACACTCGGCGGTTGCTTCCCAGC60990.318949156920998No Hit
CTCACACTCGGCGGTTATGCAGATTG60650.3171711160396545No Hit
CTCACACTCGGCGGTTGTAAGGCCAA60570.3167527534793384No Hit
CTCACACTCGGCGGTTGGATGTGGGC60500.31638668623906174No Hit
CTCACACTCGGCGGTTAGAATTCACT60310.315393075158311No Hit
CTACACTCGGCGGTTACCCGGGCACT59950.31351044363688846No Hit
CTCACACTCGGCGGTTTCTCGGTCCC59490.31110485891507084No Hit
CTCACACTCGGCGGTTGACAATACCC59420.31073879167479423No Hit
CTCACACTCGGCGGTTCGCTCTCCAG59360.3104250197545571No Hit
CTCACACTCGGCGGTTGAGCTTGCAG59050.30880386483333216No Hit
CTCACACTCGGCGGTTTGAAGATGGG59050.30880386483333216No Hit
CTCACACTCGGCGGTTACCCTAACGC59010.30859468355317415No Hit
CTCACACTCGGCGGTTAAGTTGGGGC58900.3080194350327395No Hit
CTCACACTCGGCGGTTGGCTAAGACA58890.30796713971269996No Hit
CTCACACTCGGCGGTTAGCATGTACC58730.30713041459206775No Hit
CTCACACTCGGCGGTTAGTATGATTA58500.3059276222311589No Hit
CTCACACTCGGCGGTTACAGGTCTGC58440.3056138503109218No Hit
CTCACACTCGGCGGTTGTATCACCAT58410.3054569643508033No Hit
CTCACACTCGGCGGTTGCACGATCAG58360.3051954877506057No Hit
CTCACACTCGGCGGTTCTGCACCCGT58280.3047771251902896No Hit
CTCACACTCGGCGGTCAGGGGGGCTC57610.3012733387476421No Hit
CTCACACTCGGCGGTTGCAATAGCAC57430.3003320229869309No Hit
CTCACACTCGGCGGTTTCTGGGAAAG57000.29808332422523176No Hit
CTCACACTCGGCGGTTCCAGTGGTAC56680.2964098739839673No Hit
CTCACACTCGGCGGTTCACTACCCGC56630.2961483973837697No Hit
CTCACACTCGGCGGTTTTTCCCTGGG56460.29525937694309795No Hit
CTCACACTCGGCGGTTACTGGCACAG56450.2952070816230585No Hit
CTCACACTCGGCGGTTCGCCTAGTGC55920.2924354296609642No Hit
CTCACACTCGGCGGTTCTCGGATAGA55570.2906050934595812No Hit
CTCACACTCGGCGGTTACTCTGCCTG55260.28898393853835624No Hit
CTCACACTCGGCGGTTTGCCGCAGCG55110.28819950873776357No Hit
CTCACACTCGGCGGTTCACAAGGCGG55050.28788573681752644No Hit
CTCACACTCGGCGGTTGTGGGTCCAC54990.28757196489728937No Hit
CTCACACTCGGCGGTTTGGGCTTCGT54900.2871013070169337No Hit
CTCACACTCGGCGGTTCATATGACGG54780.2864737631764596No Hit
CTCACACTCGGCGGTTGGGTCTAATG54750.286316877216341No Hit
CTCACACTCGGCGGTTGCCATGTAAG54680.2859508099760644No Hit
CTCACACTCGGCGGTTTATGGGACTG54660.2858462193359854No Hit
CTCACACTCGGCGGTTGTTTTCACAT54510.2850617895353927No Hit
CTCACACTCGGCGGTTTGTTGTCCGA54450.2847480176151556No Hit
CTCACACTCGGCGGTTCCTTCGGGGC54360.2842773597348No Hit
CTCACACTCGGCGGTTCTCGGACGCT54300.2839635878145629No Hit
CTCACACTCGGCGGTTGCGAACGGCT54240.2836498158943258No Hit
CTCACACTCGGCGGTTAGGGACTGGG54180.2833360439740887No Hit
CTCACACTCGGCGGTTGCTGAGGCGC54170.2832837486540492No Hit
CTCACACTCGGCGGTTAACGGGTGGT53830.2815057077727057No Hit
CTCACACTCGGCGGTTCGTACTTGAG53650.28056439201199446No Hit
CTCACACTCGGCGGTTGCGGACAGAA53450.2795184856112042No Hit
CTCACACTCGGCGGTTCCGTCTTGGT53390.2792047136909671No Hit
CTCACACTCGGCGGTTAAGTATCTCT53240.2784202838903744No Hit
CTCACACTCGGCGGTTGCCTATGCGG52780.2760146991685567No Hit
CTCACACTCGGCGGTTAGGCAGGGGG52580.2749687927677664No Hit
CTCACACTCGGCGGTTCCACGACCAG52270.2733476378465415No Hit
CTCACACTCGGCGGTTTTGCGGCGCG52170.2728246846461463No Hit
CTCACACTCGGCGGTTACAACATGAC52110.27251091272590927No Hit
CTACACTCGGCGGTTTCGGTAGCCAT51960.27172648292531654No Hit
CTCACACTCGGCGGTTTAGGCACGGG51930.271569596965198No Hit
CTCACACTCGGCGGTTCTAGGGTCCT51810.2709420531247238No Hit
CTCACACTCGGCGGTTACGCGGCGGC51740.2705759858844472No Hit
CTCACACTCGGCGGTTAAAGGTAGTC51480.26921630756341985No Hit
CTCACACTCGGCGGTTGGGTCTCAGG50910.26623547432116756No Hit
CTCACACTCGGCGGTTTCGGCATGAC50800.2656602258007329No Hit
CTCACACTCGGCGGTTGCTACCTTCC50720.26524186324041676No Hit
CTCACACTCGGCGGTTACTCCGTCGT50690.26508497728029823No Hit
CTCACACTCGGCGGTTCTATTTCCTT50690.26508497728029823No Hit
CTCACACTCGGCGGTTGGGATCACTG50260.2628362785185991No Hit
CTCACACTCGGCGGTTGTGTTAGGTT50230.26267939255848055No Hit
CTCACACTCGGCGGTTATGGCCACCC50220.262627097238441No Hit
CTCACACTCGGCGGTTTTCGAGCGTA50150.2622610299981644No Hit
CTCACACTCGGCGGTTAAACGTCGCG49980.2613720095574927No Hit
CTCACACTCGGCGGTTAGTCACCTGC49900.2609536469971766No Hit
CTCACACTCGGCGGTTTAGCTACTTA49830.26058757975689995No Hit
CTCACACTCGGCGGTTACACTCCCAC49810.26048298911682094No Hit
CTCACACTCGGCGGTTACCCGATCGT49590.25933249207595166No Hit
CTCACACTCGGCGGTTCGCTTCCTTT49570.2592279014358726No Hit
CGGTTAAAGAGTACCGAAAGGATGCG49380.25823429035512185No Hit
TCGTAGAGTTCCCGAGCCTGTGCGTG49060.2565608401138574No Hit
GCATGGGGAAGAGCTGCTCAGTTAGG48990.2561947728735808No Hit
CTCACACTCGGCGGTTATACTACGAG48220.25216803323053816No Hit
CTCACACTCGGCGGTTTGCAATTGCT48130.2516973753501825No Hit
CTCACACTCGGCGGTTCGCAGGGTGT48050.2512790127898664No Hit
CTCACACTCGGCGGTTTGAGCGTTTA47830.2501285157489971No Hit
CTCACACTCGGCGGTTCCTGGCTGGG47820.2500762204289576No Hit
CTCACACTCGGCGGTTACCTGCGCAA47780.24986703914879954No Hit
CTCACACTCGGCGGTTTCAGAATACA47490.24835047486765363No Hit
CTCACACTCGGCGGTTTGCTCTGCAC47100.24631095738611256No Hit
CTCACACTCGGCGGTTTGCACGGCAC47080.24620636674603352No Hit
CTCACACTCGGCGGTTCGTCGTGTAT46880.24516046034524325No Hit
CTCACACTCGGCGGTTGTTAAGGGAG46060.24087224410200306No Hit
CTCACACTCGGCGGTTAAGACGGATG46010.24061076750180552No Hit
CTCACACTCGGCGGTTGCGGTTCCAA45680.2388850219405015No Hit
CTCACACTCGGCGGTTCCATAAAACC45090.23579959805817016No Hit
CTCACACTCGGCGGTTGTCATTTTGG44990.23527664485777505No Hit
CTCACACTCGGCGGTTACTCCCCGTT44830.2344399197371428No Hit
CTCACACTCGGCGGTTCGTGACTTTT44500.2327141741758388No Hit
CTCACACTCGGCGGTTGATCGGAGGA44460.23250499289568077No Hit
CTCACACTCGGCGGTTCATTCAGCAG44250.23140679117485097No Hit
CTCACACTCGGCGGTTACGGGACATA43970.22994252221374456No Hit
CTCACACTCGGCGGTTCGGACACGTA43920.229681045613547No Hit
CTCACACTCGGCGGTTGCGGGCGCGA43830.22921038773319136No Hit
CTCACACTCGGCGGTTGCTCTGCGGG43720.22863513921275672No Hit
CTCACACTCGGCGGTTATCTCGCCAT43620.22811218601236158No Hit
CTCACACTCGGCGGTTTATGTCGACT43430.2271185749316108No Hit
CTCACACTCGGCGGTTTACCTCGCGA43050.22513135277010926No Hit
CTCACACTCGGCGGTTATTAAGGCCG42940.2245561042496746No Hit
CTCACACTCGGCGGTTACGCAGTGCG42920.22445151360959556No Hit
CTCACACTCGGCGGTTTCATGCGTTC42630.22293494932844965No Hit
CTCACACTCGGCGGTTCCAACGCACC42450.22199363356773838No Hit
CTCACACTCGGCGGTTAAATAGTATA42160.22047706928659247No Hit
CTCACACTCGGCGGTTTACACAAATA41390.21645032964354988No Hit
CTCACACTCGGCGGTTTTGGCTGGGT41080.2148291747223249No Hit
CTCACACTCGGCGGTTCACCCTGGGG40730.21299883852094192No Hit
CTCACACTCGGCGGTTGGAGCGGCGC40670.21268506660070485No Hit
CTCACACTCGGCGGTTAAGTCGCTTG40530.21195293212015165No Hit
CTCACACTCGGCGGTTCACTTCCATA40120.20980882399853157No Hit
CTCACACTCGGCGGTTAACTGTAAGC39780.20803078311718803No Hit
CTCACACTCGGCGGTTCAGATCCGAC38960.2037425668739479No Hit
CTCACACTCGGCGGTTACACGGGACC38940.20363797623386887No Hit
CTCACACTCGGCGGTTTGCTCCACAA38260.20008189447118188No Hit
CTCACACTCGGCGGTTCCGTTCTCAC37990.198669920830115No Hit
CTCACACTCGGCGGTTTCGTGGCCCG37320.19516613438746755No Hit
CTCACACTCGGCGGTTGTCTTATCCG36440.19056414622399026No Hit
CTCACACTCGGCGGTTCGAAGTGGGT35470.1854915001801574No Hit
CTCACACTCGGCGGTTGACAGGGAGG35390.18507313761984126No Hit
CTCACACTCGGCGGTTAAGAACGCAG35170.18392264057897195No Hit
GCATGCGTCTTGCCGTCTCGAGGGCT35040.18324280141845825No Hit
CTCACACTCGGCGGTTCCACCTCGGT34350.17963442433573176No Hit
CTCACACTCGGCGGTTGCTCGTGGTA34310.17942524305557372No Hit
CTCACACTCGGCGGTTACCCCACTCC34020.17790867877442781No Hit
CTCACACTCGGCGGTTACGAGAAGCC33720.17633981917324237No Hit
CTCACACTCGGCGGTTACGGAATTTG33190.1735681672111481No Hit
GCATGCGTCTTGCCGTCCAAGCCTTA32820.17163324036968608No Hit
CTCACACTCGGCGGTTCGCATGCCAT32740.17121487780936998No Hit
CTCACACTCGGCGGTTAGGGGAAGGG32230.16854781648735473No Hit
CTCACACTCGGCGGTTGGGCTGCATT31770.16614223176553705No Hit
CTCACACTCGGCGGTTTGTGTTCTAC31580.1651486206847863No Hit
CTCACACTCGGCGGTTCGCAGACGGA31520.1648348487645492No Hit
GCATGCGTCTTGCCGTAAATATCCGA31020.1622200827625735No Hit
CTCACACTCGGCGGTTGCCAGCGATT30560.15981449804075584No Hit
GCATGCGTCTTGCCGTGTTGCTGGGG30240.15814104779949137No Hit
CTCACACTCGGCGGTTGTTAGTTCCG30230.15808875247945187No Hit
CTCACACTCGGCGGTTAGTTGTACAG29970.1567290741584245No Hit
CTCACACTCGGCGGTTTTTTCTCTCG29330.15338217367589557No Hit
CTCACACTCGGCGGTTAGACCCCGTG29110.15223167663502624No Hit
CTCACACTCGGCGGTTACTACTCAGG28710.15013986383344569No Hit
CTCACACTCGGCGGTTCCCTAAAGTG28250.14773427911162804No Hit
CTCACACTCGGCGGTTTCTCATTGTT27630.14449196926917812No Hit
CTCACACTCGGCGGTTCGTTCCGTTA27570.14417819734894105No Hit
CTCACACTCGGCGGTTGGTAGAGCTC26420.13816423554439689No Hit
GCATGCGTCTTGCCGTCATGACTTAA26200.13701373850352758No Hit
TCGTAGAGTTCCCGAGGGCGTAGCTA25960.13575865082257924No Hit
CTCACACTCGGCGGTTCGGCACCCAG25830.13507881166206556No Hit
GCATGCGTCTTGCCGTACCCCTGCAA25620.13398060994123576No Hit
TCGTAGAGTTCCCGAGGCCCCAGGTC25360.13262093162020838No Hit
TCGTAGAGTTCCCGAGCCGATACGTA24320.12718221833609888No Hit
CTCACACTCGGCGGTTACACATTCGA23780.1243582710539651No Hit
CTCACACTCGGCGGTTGAGACTTGGG23700.12393990849364898No Hit
GGGATGAAGGCCGAATACCTCCTAAC23440.12258023017262162No Hit
GCATGCGTCTTGCCGTCGACCAGCTT23430.12252793485258212No Hit
TCGTAGAGTTCCCGAGCTGTGGGCTA22320.11672315432819601No Hit
CTCACACTCGGCGGTTAATGTCGGCA22220.11620020112780088No Hit
GCATGCGTCTTGCCGTTCCGAATGTG22130.11572954324744524No Hit
TCGTAGAGTTCCCGAGACCCGTTGGC21590.11290559596531147No Hit
TCGTAGAGTTCCCGAGATGGTTAACC21230.11102296444388896No Hit
CTCACACTCGGCGGTTGTGAGACCCT21200.11086607848377042No Hit
CTGATGGCTCAAACACAGCGACCTCG20450.10694392948080683No Hit
TCGTAGAGTTCCCGAGGGGAATGACG19990.10453834475898918No Hit
GCATGCGTCTTGCCGTCCCGGGACCC19430.10160980683677637No Hit
TCGTAGAGTTCCCGAGACCTTCCTGT19410.10150521619669735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)