FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n01_CITE-seq_test_TCells_GD3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n01_CITE-seq_test_TCells_GD3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9701512
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTATGTTGTCTTTGCTTTATGTT405350.41782146947815973No Hit
TACCTATGTTGTCTTTACGCAAGGCT332890.3431320808550255No Hit
TACCTATGTTGTCTTTCAGCTCTCTC305610.3150127526513393No Hit
TACCTATGTTGTCTTTCTGCAGCCTG234560.2417767457278824No Hit
TACCTATGTTGTCTTTGCCCTATGTT213210.21976986680014415No Hit
TACCTATGTTGTCTTTAAGCCGTACG205620.21194634403379595No Hit
TACCTATGTTGTCTTTATCTGCTACG205280.21159588319841277No Hit
TACCTATGTTGTCTTTACATGTAAGC204770.21107019194533802No Hit
TACCTATGTTGTCTTTACCGCTATCT201980.20819435156087007No Hit
TACCTATGTTGTCTTTCTCGCCCCGT193120.19906175449764946No Hit
TACCTATGTTGTCTTTCCACCGGGAC191030.19690745112720576No Hit
CCTACCACACGGATAGGGTATATGGA187190.19294930522170153No Hit
TACCTATGTTGTCTTTGGCGCCCGGT178090.18356932403938683No Hit
TACCTATGTTGTCTTTCGCGCTGCGT175130.18051825323722734No Hit
TACCTATGTTGTCTTTCTGTAACGAC174340.1797039471785429No Hit
TACCTATGTTGTCTTTGAAGACTTGC173320.17865256467239332No Hit
TACCTATGTTGTCTTTGTGGGATAGC173060.1783845652100415No Hit
TACCTATGTTGTCTTTCCAGCTCGAT170580.17582826264607002No Hit
TACCTATGTTGTCTTTCATTGACGAG167910.1730761143211491No Hit
AGCCTAGTGCCAGAGCAAAGTAGCTT167430.17258134608296108No Hit
CTAGCCTAGTGCCAGAGATTATACGG165630.170725965189756No Hit
TACCTATGTTGTCTTTCGAAAGTAGG165550.17064350381672466No Hit
TACCTATGTTGTCTTTTATGCCATAG164910.16998381283247394No Hit
TACCTATGTTGTCTTTCACTCATGTT164120.1691695067737895No Hit
TACCTTATCAAGCCTATTAACGCACC158300.16317044188575966No Hit
TACCTATGTTGTCTTTTTTTGGTTTG157850.16270659666245837No Hit
TACCTATGTTGTCTTTTGTCGTGGTG155490.16027398615803393No Hit
TACCTATGTTGTCTTTTTCCAAGCTG154150.158892758159759No Hit
TACCTATGTTGTCTTTCGGTGGGGGG153540.15826399019039505No Hit
CACAGGCTCTACTCATTGGGGCCCTG152550.15724353069913227No Hit
TACCTATGTTGTCTTTCGCTCATGTC152200.15688276219212013No Hit
TACCTATGTTGTCTTTCTCCTCATGC151730.15639830162556104No Hit
TACCTATGTTGTCTTTATGAAAGGCA151630.15629522490927186No Hit
CAGAATCCATCTCCCACCTGCGTAGT149600.15420276756860168No Hit
TACCTATGTTGTCTTTGCAAAAGCGG147080.15160523431811454No Hit
TACCTATGTTGTCTTTCAAATAACCG145230.14969831506676484No Hit
TACCTATGTTGTCTTTTTCAATCATG144510.1489561627094828No Hit
TACCTATGTTGTCTTTATTAGGCGCT142660.14704924345813314No Hit
CAAGAAAGTCAGCTATAGGGCTTCTT134890.13904018260246445No Hit
TACCTATGTTGTCTTTAAGGGCTGAG134140.13826710723029564No Hit
TACCTATGTTGTCTTTCCACTGTCAA125280.129134510167075No Hit
TACCTATGTTGTCTTTGGCCCAAACG123890.1277017438106555No Hit
GCAATCAGTGCCTTGGAGCGCTTGGC123190.1269802067966313No Hit
TACCTATGTTGTCTTTTCTAGCGGCA121740.12548559441043827No Hit
CTAGCCTAGTGCCAGACCCTAGAGCG121140.12486713411270324No Hit
CTAGCCTAGTGCCAGAGTCAATTATT121120.12484651876944541No Hit
CGTAGCGGTTAGGGTGCTCCGTACGG120070.12376421324840911No Hit
TACCTATGTTGTCTTTTTTCATCTCC109290.11265254323243636No Hit
CAGAATCCATCTCCCACCCTTACGTG103340.10651947861323059No Hit
TACCTATGTTGTCTTTCCCTATCTGA103090.10626178682250767No Hit
TGACTTTTACTTCCTCTAGATAGTCA102820.10598347968852691No Hit
CAGAATCCATCTCCCAAAAGCGGCAG101790.10492178951074843No Hit
TACCTATGTTGTCTTTACATGCTCCT100060.10313856231894573No Hit
TACCTATGTTGTCTTTAACAGCGGGC98580.10161302691786599No Hit
TACCTATGTTGTCTTTCTGTATTGCG98550.10158210390297925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)