FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n01_CITE-seq_test_TCells_GD2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n01_CITE-seq_test_TCells_GD2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262195
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCG31001.1823261313144797No Hit
TAGCCGGCAAGCCGCTTATTACAGGA16430.6266328495966742No Hit
ACACGACGCTCTTCCGATCTACACGA16120.6148095882835295No Hit
TAGCCGGCAAGCCGCTTGCTGAGGCA11290.4305955491142089No Hit
TCTGATGGCTCAAACACAGCGACCTC8670.3306699212418238No Hit
GATCAGTTCACCCGAGACTGCACGAA7920.302065256774538No Hit
AGTCTCTCAGCTGGTACACGGCAGGG7390.28185129388432273No Hit
TGATGGCTCAAACACAGCGACCTCGG7010.26735826388756456No Hit
GTCTCTCAGCTGGTACACGGCAGGGT4720.1800186883807853No Hit
GATGGCTCAAACACAGCGACCTCGGG4410.16819542706764049No Hit
CTATCTATACGTGGTGTTGCCTGATG3720.14187913575773756No Hit
GACCTGGTTCACTATCGGTCGATGAT3720.14187913575773756No Hit
CAGTCCTAGTGTGACCGTCCACCCTC3670.13997215812658517No Hit
ACTGATGGTGAGCGATTAGGTGGATG3510.13386982970689754No Hit
AGAGCTTGTGAAATCACCAGTAACAC3470.13234424760197563No Hit
CGATTGATCTCAACTTCCGGGGAACC3390.1292930833921318No Hit
GGTGAAGTCCTTTCTCCGCCACAGGG3270.12471633707736608No Hit
TATCTCAGTTCAGCGCACCTGACTGT3110.11861400865767843No Hit
TGCGTGGTCTCATTCACATGCTTCCG3020.11518144892160415No Hit
CAGTCCTGTTTGCTACCCATGCTTTC2830.10793493392322509No Hit
GACCTGGAGGTGTTAAAACGGGTAAC2760.10526516523961173No Hit
CGTTCTGAGAATTGTGTCTAATGGGT2760.10526516523961173No Hit
GATCTAGGTTCTGTTTACTTTACGTG2740.10450237418715078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)