FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n01_CITE-seq_test_TCells_GD1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n01_CITE-seq_test_TCells_GD1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5112139
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCAGTCATCGATGAGGCGGGCTG256510.5017664817016908No Hit
AATCCAGTCATCGATGCGCAAACAGC241960.473304814286153No Hit
AATCCAGTCATCGATGGCAGCCCGTA164620.3220178481062428No Hit
CACCTTGTTACGACTTTTACTTCCTC99730.19508467981797836No Hit
AATCCAGTCATCGATGGCGGATGACG98740.19314811275671495No Hit
GACTAACTCGAGAACGTGATTCCTCA97570.19085944259340365No Hit
CTACACCTCTCATGTCTCTTCACCGT94820.1854800896454498No Hit
AATCCAGTCATCGATGAGTCATCTAT86200.16861826331404525No Hit
AATCCAGTCATCGATGGGGTGCCTAC84660.16560582566319107No Hit
AATCCAGTCATCGATGTTCGGACAGG83720.1637670650191632No Hit
AATCCAGTCATCGATGCAGTACACAC83530.16339540063366823No Hit
AATCCAGTCATCGATGCATTCATAGA80630.15772262843400778No Hit
AATCCAGTCATCGATGTCTGCCGCGA80420.15731184148161856No Hit
AATCCAGTCATCGATGTACGCGGCCA80270.15701842222991197No Hit
AATCCAGTCATCGATGCGTTAAGGAT80180.15684237067888804No Hit
AATCCAGTCATCGATGGCCAGGCCGG79620.15574693880585014No Hit
AATCCAGTCATCGATGAGGCACGTCC79240.15500361003486016No Hit
GGTGCGTGACGGGCGAGGGGGCGGCC76870.15036758585789628No Hit
AATCCAGTCATCGATGCGCTCGGTCG75880.14843101879663287No Hit
AATCCAGTCATCGATGTCCGGACGTG75860.14839189622973867No Hit
AATCCAGTCATCGATGCCTCTGCGCG75100.14690523868775868No Hit
TGACTTTTACTTCCTCTAGATAGTCA74610.14594673579885054No Hit
AATCCAGTCATCGATGGCACCGACTC73030.14285605301420795No Hit
AATCCAGTCATCGATGGTATGATCAC72110.14105641493707427No Hit
AATCCAGTCATCGATGTTAATGGTAG71960.1407629956853677No Hit
AATCCAGTCATCGATGTGGAATACAT71610.14007835076471903No Hit
AATCCAGTCATCGATGGTTCGCCGGG68350.1337013723609628No Hit
AATCCAGTCATCGATGAATTCAACCG68020.13305585000720832No Hit
AATCCAGTCATCGATGCGTTGGTAGC66990.13104103781215654No Hit
AATCCAGTCATCGATGCCGAGGCGTC66750.130571567009426No Hit
GACTAACTCGAGAACGAGGCAGATTT66720.13051288315908469No Hit
AATCCAGTCATCGATGCGACGGTACT65180.1275004455082305No Hit
AATCCAGTCATCGATGGCGGTACGTT64810.12677667802068762No Hit
AATCCAGTCATCGATGGTAATGGGGA64560.12628764593451No Hit
AATCCAGTCATCGATGCGTTATACAG64130.1254465107462845No Hit
AATCCAGTCATCGATGCCTCTTCCGC63750.1247031819752945No Hit
AATCCAGTCATCGATGAGGCCCGGGG63210.12364687266915084No Hit
AATCCAGTCATCGATGATCGCCATGC63210.12364687266915084No Hit
AATCCAGTCATCGATGCGGAAGGACG62970.12317740186642029No Hit
AATCCAGTCATCGATGTAAGTTTCGT62580.1224145118119832No Hit
AATCCAGTCATCGATGAGTATCGGAT62230.12172986689133453No Hit
AATCCAGTCATCGATGCCTTTGCGGT61340.11998891266454217No Hit
AATCCAGTCATCGATGCCAGCTCATC60430.11820883587085564No Hit
AATCCAGTCATCGATGGTCATATCGG60140.11764155865088959No Hit
AATCCAGTCATCGATGACTCCGTCAC59790.1169569137302409No Hit
GGTCCGTCTTGCGCCGGTCCAAGAAT58880.11517683693655434No Hit
TGATTTAATGAAAACATTCTTGGCAA58560.11455087586624699No Hit
TGATTAATGAAAACATTCTTGGCAAA58480.11439438559867013No Hit
AATCCAGTCATCGATGGTTTCACGCT56660.11083423201129704No Hit
GTACGAAACCCCGACCCAGAAGCAGG56010.10956274858723519No Hit
AATCCAGTCATCGATGCGTGGTTTGG55820.10919108420174022No Hit
AATCCAGTCATCGATGTATTTAGGGT55540.10864336826522127No Hit
AATCCAGTCATCGATGTTTAACAGGC53830.1052983887957663No Hit
AATCCAGTCATCGATGTCACGGCAGC52030.1017773577752874No Hit
TGCACCTGCGGCGGCTCCACCCGGGC51680.10109271285463874No Hit
AATCCAGTCATCGATGTTGCTCTGCC51150.10005596483194217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)