FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n01_CITE-seq_test_BCells_PI1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n01_CITE-seq_test_BCells_PI1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51155261
Sequences flagged as poor quality0
Sequence length26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCTAGTAAGTGTAGCGGCTGGGC8826241.725382654190739No Hit
GATCTAGAGTGCCATTCGCGCCAATA8686671.6980990479161078No Hit
ACGCCGACAGGTGCCTCGTCGCCCTT8098131.5830492977056652No Hit
AGTGTCACACACCGACACGTGTCCTG6253371.222429497525191No Hit
CAGTAACTCATCATTCGCGCTGGGCT6186861.2094279022445023No Hit
CCTAGCTGTCAGATAACAGCACGCTG6185931.2092461027615518No Hit
TGCGGGTTCTAACGGTAGCCCCGGAT5716111.1174041317079781No Hit
GTGTTAGTCGTCCAGGGCGCGCAATG5398211.055259985869293No Hit
TTCCCAGTCGTCCGTTTTCTTTGGGC5264461.0291140924879654No Hit
GTGTTAGTCGTCCAGGGGCTCCGTAG5159821.0086587184063043No Hit
ACTGTCCGTTCTCATTCTCGGACCGT5141961.0051673864003938No Hit
CCTAGCTGTCAGATAAGTAAAATTGT5062490.9896323273572977No Hit
CAGTAACTCATCATTCTCGGGATCTT4972450.9720310096746452No Hit
CATATTCTCAACACCAGCCCGCCGGC4968180.9711962959195927No Hit
CAGTAACTCATCATTCTCTCGGCTCC4942820.966238839051178No Hit
CAGCCGAGTACCTACAACTCTCACCG4939820.9656523891061762No Hit
TGCGGGTTCTAACGGTCTTATTGTCA4938350.9653650286331253No Hit
ATTACCTCGGCATCGGAAGGATCGCT4874690.9529205608001882No Hit
ACAGCTAGTAAGTGTAGAGACAGCAA4762560.931001016689173No Hit
GCCAAATAGCGCCTCAACTGAGCAGT4590020.8972723255189725No Hit
CTCGGGACATTATCTCAACCGGCGGT4400090.8601441795009119No Hit
CTCGGGATTATCTCCCCCGCGTCCTT4349530.8502605430944825No Hit
CTCACACTCGGCGGTTTGTCTGCTTC4274190.8355328301423387No Hit
ACGCCGACAGGTGCCTTGCGGACGGA4249970.8307982242530246No Hit
CTCACACTCGGCGGTTGGTGCCCCTC4208470.8226856666805002No Hit
GTGTTAGTCGTCCAGGATCATAGTGG4070220.79566009838167No Hit
CTCGGGACATTATCTCAACCGAACTA4060380.7937365425620642No Hit
TTCCCAGTCGTCCGTTATCGTTCACC4014310.7847306262399872No Hit
GTGCTTCCATCACCCTCCGCTCAGAG3844830.7516001140136886No Hit
TGCGGGTTCTAACGGTTCCGCCGCGG3739380.7309863984468773No Hit
CCCAGTTCATGGTTGTGTTGAGCCGT3688530.7210460718790976No Hit
CTGCGGAAGAGACGAACACAAAGGGT3635450.7106698175188667No Hit
CTCACACTCGGCGGTTCAGACCAGGG3612260.7061365594440032No Hit
CAGCCGAGTACCTACACCGCAGATCG3512100.6865569506135449No Hit
GCCAAATAGCGCCTCACTGGCCTCGG3498150.6838299583692868No Hit
CCTAGCTGTCAGATAAGCTCCGTGAC3459140.6762041542511141No Hit
ACAGCTAGTAAGTGTAGTATCGGGGC3290980.6433316800006161No Hit
TTCCCAGTCGTCCGTTTCCGAGCGGG3262230.6377115346943494No Hit
CTCACACTCGGCGGTTACTCCGTATG3133900.6126251608803247No Hit
ACGCCGACAGGTGCCTCCGGTGGTGT3124060.610701605060719No Hit
CATATTCTCAACACCAGATTTCCATT3106150.6072004988890586No Hit
GTGTTAGTCGTCCAGGAGCCCGGGCC3069220.5999813000660871No Hit
GTGTTAGTCGTCCAGGGCCGACAGCG3026120.591555969189562No Hit
TGCGGGTTCTAACGGTCGTGTGGTAC3024910.5913194343784113No Hit
CAGCCGAGTACCTACACCGCCGCCAT3013710.5891300212504047No Hit
CGCGTTTGTGCGATAGGAGACCTATC2965440.5796940416353267No Hit
GCCAAATAGCGCCTCATCGGTATCAG2873940.5618073183127734No Hit
CACATAGTCGTCCGTTACAGGACCGA2799340.5472242630137298No Hit
CATATTCTCAACACCAGCGTACCCTC2727150.5331123225038379No Hit
CGCGTTTGTGCGATAGCGCATCGTAC2704540.5286924447516747No Hit
CTCGGGACATTATCTCTGTTCAGCTT2591740.5066419268196091No Hit
TACTTACCAGGTGGATCAACGTGTCA2534400.4954329135374756No Hit
CATATTCTCAACACCATTGCGTCTCC2514350.49151347307171395No Hit
CACATAGTCGTCCGTTATTCCTTTGC2407830.47069059035785193No Hit
ACTGTCCGTTCTCTTTCTTATATGGG2397100.4685930543878957No Hit
GTGTTAGTCGTCCAGGGTGGATGGGT2383190.46587388147623765No Hit
GCAAATAGCGCCTCACCCACTGGGAT2359380.4612194237460738No Hit
TAAGCGTCATTCTCATATTCCCCAAC2359310.46120573991402364No Hit
GTGTTAGTCGTCCAGGTAAGACTTAA2327140.45491704167045494No Hit
CATATTCTCAACACCAATGCCACCCG2312400.45203561760734634No Hit
CACATAGTCGTCCGTTCTTCTTCGGG2281910.4460753313329786No Hit
GTGTTAGTCGTCCAGGATCTGAGGGG2281530.44600104767327836No Hit
TTCCCAGTCGTCCGTTCGTCTTTACA2276290.4449767151026754No Hit
CACATAGTCGTCCGTTTAAGCGAGCA2268230.4434011195837707No Hit
GTGTTAGTCGTCCATAGGCCCAGCTT2249020.4396458851026095No Hit
CACATAGTCGTCCGTTGTTTTTCACA2246450.439143492983058No Hit
CTCGGGACATTATCTCTGTCCGCGTT2203670.43078071676733304No Hit
GTGTTAGTCGTCCAGGATGCTGGGTC2188280.4277722285494741No Hit
GCCAAATAGCGCCTCACTGCAATTAA2171250.4244431476950142No Hit
CATATTCTCAACACCACCGGCTTATA2168200.4238469235842624No Hit
CACATAGTCGTCCGTTCAAGGGTCGC2124310.41526716088888693No Hit
CTGCGGAAGAGACGAAACGTACCAGT2121140.41464747878033503No Hit
CACATAGTCGTCCGTTGACCGTCTTT2116180.4136778815379321No Hit
CATATTCTCAACACCAATATTGCCTT2038780.3985474729568871No Hit
GTGTTAGTCGTCCAGGAGGCTCATCG2028960.39662782680358133No Hit
CGCGTTTGTGCGATAGAAAAGCGCCA1966230.3843651584535948No Hit
CACATAGTCGTCCGTTATTCACATGA1905100.3724152634076093No Hit
CTCGGGACATTATCTCAGAAAAGCCT1899360.3712931891795059No Hit
CACATAGTCGTCCGTTCAATGTTCAT1891090.36967654216445106No Hit
CATATTCTCAACACCATCATAGTCCA1890010.36946542018425044No Hit
GTGTTAGTCGTCCAGGTGGGGCACCG1864270.3644336796561355No Hit
GAAATGAAGGCAAAGACTCCGGGTGC1863620.3643066155013851No Hit
TGTATTCGTTACGCGCAATTGGCTGG1843140.36030311721017316No Hit
CACATAGTCGTCCGTTCCCAAGCAAC1839350.35956223544632093No Hit
CTCGGGACATTATCTCCCCGGAGCCT1829240.35758589913166505No Hit
GAAATGAAGGCAAAGATCACCGTTTT1816670.3551286738621077No Hit
TAAGCGTCATTCTCATTACCAGGTCG1814970.35479635222660677No Hit
GTGTTAGTCGTCCAGGCGGCCAGCGG1812450.3543037342728053No Hit
TGTATTCGTTACGCGCAGGCCAGTCT1805880.35301940889325145No Hit
GCCAAATAGCGCCTCAATATGTAGGT1805860.35301549922695147No Hit
GAAATGAAGGCAAAGAAAACAGCATG1780270.34801308119608654No Hit
CACATAGTCGTCCGTTGAACAACGTT1755850.34323937864377235No Hit
ATCCACCGTGCCTGTGCGGTTGGAGA1754450.34296570200277154No Hit
GCCAAATAGCGCCTCAGTGTCTTTGG1748020.3417087442873178No Hit
CACATAGTCGTCCGTTTCGTCCATAC1745210.34115943617216615No Hit
CAAGTTGGTAGCCTATAGGGACTGGA1728470.3378870454790564No Hit
GCCAAATAGCGCCTCAGGGTTTCCTC1714810.33521674339614843No Hit
CATATTCTCAACACCAAGAATACTTT1698410.3320108170301389No Hit
GTGTTAGTCGTCCAGGCTACAGGCTG1631160.3188645640963497No Hit
GCCAAATAGCGCCTCATGGAATACGC1614020.31551398007723974No Hit
ACTGTCCGTTCTCATTTCGGGTTAGG1607800.3142980738579361No Hit
AAGCGTCATTCTCATGTTCTGGTGTT1607020.31414559687223564No Hit
CATATTCTCAACACCATTATTGGTCT1590230.3108634320133759No Hit
ATCCACCGTGCCTGTGCCCGGAATTT1581400.30913731434192077No Hit
TATTACCTCGGCATCGTTTACCTACT1579710.3088069475395698No Hit
CATATTCTCAACACCAGATATACCTT1569640.3068384305575139No Hit
ATCCACCGTGCCTGTGGTTGTGACGT1543660.3017597740337988No Hit
CAAGTTGGTAGCCTATTCACGCCCGA1538280.30070807379909564No Hit
GCAATAGCGCCTCACCACGCATTGTT1534750.3000180176971436No Hit
CGCGTTTGTGCGATAGCCAAACACTG1530960.2992771359332914No Hit
GATGAGGGTTTGGGCCCTTAGTATAG1501890.2935944359662245No Hit
TACTTACCAGGTGGATAGCCTCACGT1490420.29135224234316776No Hit
GATCTAGAGTGCCATTTGCTCTGTCG1447100.2828839051373426No Hit
CATATTCTCAACACCAGCACCGTACC1438100.2811245553023373No Hit
ACTGTCCGTTCTCATTCCCAAAGGGA1435180.28055374402253563No Hit
AGTGTCACACACCGACACCCTCACCC1417510.27709955384647533No Hit
TAAGCGTCATTCTCATGGCAGTGGTT1365070.26684840880784483No Hit
ACACCGGCATGCCTAATACGTTGGGC1359880.2658338504029918No Hit
GCCAAATAGCGCCTCAGTAGGTTCCG1309840.25605186532036267No Hit
CTCGGGACATTATCTCTAGGGATGGG1275960.24942889060814294No Hit
AGTGTCACACACCGACGGCGGACGAG1253080.24495623236092962No Hit
CCCAGTTCATGGTTGTTTGGGCGTGC1253060.24495232269462958No Hit
GTGTTAGTCGTCCAGGCCGCGCAGGC1240650.2425263747554724No Hit
CACATAGTCGTCCGTTCGATGGACTT1230160.24047575478111627No Hit
GTGTTAGTCGTCCAGGGCTCTGCATC1180300.23072895669518723No Hit
CGCGTTTGTGCGATAGCCGTTTCCGT1173550.2294094443189333No Hit
CGCGTTTGTGCGATAGGGTTAGCCGC1166640.22805865461227925No Hit
CATATGGGTCCGACGTGGTGTGCCTC1154640.2257128548322723No Hit
GTGTTAGTCGTCCAGGACGCTGGTCA1149090.22462792243401905No Hit
CACATAGTCGTCCGTTTAGATAGGGT1103580.21573147676834256No Hit
CATATGGGTCCGACGTCTGCCTCAAC1035720.20246597901240304No Hit
GCCAAATAGCGCCTCACTGGCTCTTT1026990.20075940967244796No Hit
GCCAAATAGCGCCTCACGACCGCCCT1001480.1957726303067831No Hit
CGCTTTTGTGCGATAGGACTTTGCTC968540.18933340991066394No Hit
GTGTTAGTCGTCCAGGCTGGTACTCA916050.17907249070628337No Hit
TGTATTCGTTACGCGCCTGACTAGGT891070.17418931749756883No Hit
GTGTTAGTCGTCCAGGGACTTATGGA884230.17285221162296485No Hit
GAAATGAAGGCAAAGATCGGCTTGCC871720.17040671535230756No Hit
GTGTTAGTCGTCCAGGTGGAGTACCG867980.16967560775420537No Hit
CACATAGTCGTCCGTTGCGCGTTATG863250.16875097167425263No Hit
TAAGCGTCATTCTCATAGGCTTACGG851330.1664208105594457No Hit
ACTGTCCGTTCTCATTTTTTTGCGGC808050.1579602926862205No Hit
CAGTAACTCATCATTCGGCACAGGGT807130.15778044803641994No Hit
GATCTAGAGTGCCATTTTGGTGCGAT795230.155454196587913No Hit
CACATAGTCGTCCGTTCGCAGATAAG793190.15505541062531183No Hit
ACACCGGCATGCCTAAACAGGCAGCG765040.14955255530804545No Hit
TTCCCAGTCGTCCGTTCGGTGCCGCG747190.14606317813528505No Hit
ACTGTCCGTTCTCATTCGAATAGGAA743320.1453066577062328No Hit
CCTAGCTGTCAGATAAACCGAACATC723590.1414497719012713No Hit
TATTACCTCGGCATCGGCGTCTTAAT630130.1231799012813169No Hit
CACATTTAGATCCTGTCGTGTTCCCT630080.12317012711556687No Hit
CTCGGGACATTATCTCACTCATCCGT619840.1211683779699609No Hit
TGCGGGTTCTAACGGTTCCACGGGCG575790.11255733794418524No Hit
CATATGGGTCCGACGTCGCGAATCAC571760.1117695401847329No Hit
CAGCCGAGTACCTACACTGTTGGCAC571470.11171285002338274No Hit
TCGTAGAGTTCCCGAGTGGTCGTCCA567080.11085467827053018No Hit
ACAGCTAGTAAGTGTAAAGCGGATAT565530.11055167913227928No Hit
CTCGGGACATTATCTCACTGGTGCTC553890.10827625334567251No Hit
GCCAAATAGCACCTCAACCAAAAATA552490.10800257670467169No Hit
ACAGCTAGTAAGTGTACCTCCGCGAA542920.1061318013801161No Hit
TGCGGGTTCTAACGGTGGTCCCGTTA541030.10576233791476501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)