FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n01_CITE-seq_test_BCells_GD2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n01_CITE-seq_test_BCells_GD2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6990888
Sequences flagged as poor quality0
Sequence length26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCG368580.5272291588708045No Hit
TGGCCAGCAGATCTGTCTGCGGCGGG344380.49261266952066746No Hit
TACGGTACAACACCTACTTGGCTGGG335190.4794669861682808No Hit
AGTGTCATCGAGAACGCCCACTGCTA326290.466736128514718No Hit
GGTATTGAGAGCCTAGCGCGTTCCGT316970.4534044888145826No Hit
ATTCTACAGCCAGGATGTGGTGGGTC308760.4416606302375321No Hit
TGGACGCAGGCAGTCAGGCAACACGC305000.4362822004872629No Hit
GCAGTTACATCAGTCATGTCGTTGGG296280.4238088208536598No Hit
GATCAGTTCACCCGAGACTGCACGAA275940.3947138045982141No Hit
AACTGGTCCCGGTTTCTTATATGGGG264620.3785212980096377No Hit
TTGCGTCCAAACAACAGCCTGAGCCC260150.3721272605139719No Hit
AGCATACTCTGGCGACACGAGACTGC252880.3617280093744886No Hit
GGTGAAGAGGCGATACTTAGATTACG249220.3564926229686415No Hit
TATGCCCTCAGTTCGATGCTTTCAAG242090.34629363251134904No Hit
ACACGACGCTCTTCCGATCTACACGA240120.3434756786262346No Hit
GAAGCAGCAAGTAGTATGCGAACGTG232160.33208942841023914No Hit
GTGCGGTAGGGTTCCTCAACCACTCG230810.33015834326054144No Hit
GCGCCAAAGCTAAGATCCGTGCGCGC224430.3210321778864144No Hit
CATCCACCAAATACAGCACTCTCGCG221510.3168553122292905No Hit
ACTATCTCAATGAAACAGTAGTCCCC219880.314523705715211No Hit
ATGGGAGAGCAATCTCGCAATAACAG210090.30051976229629196No Hit
GAGTCCGTCTGACCTCAGTCGAGTCG200090.2862154278540866No Hit
CGTCAGGCAATACGCTGTCTGTTGGG199740.28571477614860946No Hit
ACGCAGCTCGCACTCTTAATCAGGCC198340.2837121693267007No Hit
AGACGTTTCCGCATCTCGACGAGAGT197910.28309708294568586No Hit
GGGAGATAGCGATAGCCGGGACCCCC196050.28043647673943567No Hit
GGATTACAGGAACTGCCACAGAATCG185370.2651594475551604No Hit
CGCCAAGGTCGATTGTGATACGCCAA179480.25673419456870145No Hit
TTAGGCATCGAGAGCACAATTACCTC178050.25468867474346607No Hit
CAACCAATCACAGTACCGCACAGTAC173350.24796563755562956No Hit
TCTGAGAGTCTAGCCGAGGTGGAGCT172250.24639216076698697No Hit
CTACATTCAGAGTGTGTGCATCAGCC169660.2426873381464558No Hit
ATTCTACAGCCAGGATTCGCTGTGCG163450.23380434645784626No Hit
CTGAAGTTCTTAACCTCCGTCTGCCT159480.22812552568429073No Hit
GCAGTTAAGATTACCCCTACGTACCG158700.22700978759779872No Hit
CCCTCCTAGATCACGGTAGAGCAATT158410.22659496189897477No Hit
CTACACCTCGTGACATGTGTGCGGAT155110.22187453153304704No Hit
CAACTAGGTCCAGTTAGTAGTTCGCG154940.22163135784752952No Hit
ATGTGTGGTCTCTTATTCCGTCTGCC153440.21948570768119874No Hit
GGGATGAAGAAGGGTAGTGGCCACGC153080.21897075164127935No Hit
TTGGCAACAAACTGTCCCCTACGAGC149640.2140500605931607No Hit
TCGGTAATCGCATGATTCAGTAGCGG148460.2123621491289805No Hit
ATCCACCCACATTCGATCAACATTGG147880.2115324977313326No Hit
GAACGGATCTCCAACCGTGTCCGAAC145400.20798502278966563No Hit
CAGCTGGCAAGAGTCGGACGACTGCG143370.20508124289789795No Hit
AGGTCATAGATGCCTTTTCCTTAGTA142440.20375093979477285No Hit
CTCTGGTGTGGCTCCAAGCCAAATGC139420.19943103079322683No Hit
AGAGCTTCATAAAGGTCCCTTCGAAT134040.19173529886332036No Hit
CGAGAAGCATAGACTCACTCTGTGGC128660.1840395669334139No Hit
TCCACACAGAATGTTGTGACCTCCAA128110.1832528285390926No Hit
CTTAGGATCCTTGGTCGATCGCAGTG126810.1813932650616059No Hit
AGGCCACTCGACGGAACCACGGCCAA126360.18074957001170666No Hit
TCACGAAAGTGAATTGTATCTTCGTC123400.17651548701681388No Hit
CACACCTAGGCGATACTACCCGATCA122020.17454148886378956No Hit
AAGCCGCTCACAACGTTGACACTGCC119820.17139453528650436No Hit
GCTGCGATCGAATGCTCACAGCTGGG119760.17130870927985115No Hit
CGCGTTTGTGTAATGAGGCCACGTGC118370.1693204067923846No Hit
GCTCTGTCATCATCCCCGCAGCAGCT118260.16916305911352034No Hit
ACGAGCCAGACCTTTGCCATACTATG116780.16704601761607396No Hit
AAACGGGTCGTAGGTTCCTATTCAGC115540.1652722801452405No Hit
ACGGGTCCAGTATAAGCGACTGCCTT115300.16492897611862756No Hit
CGGACACAGATGTAACGGTGGTCCGG113540.16241141325679942No Hit
CGATGGCGTTACGACTCCAGGTGGCC112330.1606805887892926No Hit
TACTCGCCAAGGTCGATTGTGCCCCA108610.1553593763767922No Hit
GACCTGGTTCACTATCGGTCGATGAT107180.15331385655155683No Hit
CAGTCCTAGTGTGACCGTCCACCCTC106030.1516688580907032No Hit
CTAAGACTCCCAAGATGGTTCCGGTC105580.151025163040804No Hit
AACTTTCTCCTACAGACAGGCAAGGG104350.14926572990441273No Hit
TCTGATGGCTCAAACACAGCGACCTC104280.1491655995633173No Hit
CTAGTGACATCAGTCAGCAGCTACCT102060.1459900373171477No Hit
GTGAAGGAGTCAATAGTCACACGCAA100490.14374425680972147No Hit
GTGTGCGGTGAGCGATATATCGCCCC98190.14045425988801422No Hit
GGGCATCGTCCGAACCACATTAACCG97090.13888078309937166No Hit
AACCGCGCAATCTACGCGATATGTCA96530.13807974037060813No Hit
TGATGGCTCAAACACAGCGACCTCGG95550.136677915595272No Hit
CTGGTCTAGGATGTATCTTCGGTCTG93040.13308752765027848No Hit
TGGCTGGAGGCTATCAACAGCGGGTA91890.13144252918942487No Hit
TACCTATCATCTACGATCATCCGGAC91390.1307273124673146No Hit
CCATTCGCAGCCAGAAAGATCGAGTT90320.12919674868199862No Hit
ACGCAGCCATCGGTTACGCCCCACGC90070.12883914032094348No Hit
TACCTATAGAGGTACCCGGGCGGCAT89300.1277377065688937No Hit
AGCCTAATCCGCTGTTGACAGGACAA88680.12685083783347695No Hit
CGTCAGGCAGTCTTCCAGTGCCGGTG86980.12441910097830206No Hit
CGGCTAGTCAAACGGGGGCCCTATAG84890.12142949507988113No Hit
ACTGATGAGGCTAGGTGCTAAGTTGG84210.12045680033781117No Hit
CCCTCCTACTCGTCGCGGCGTAGCGT83430.11934106225131914No Hit
CCTTCCCTCAGGATCTCGAATCTTCC83240.11906927989691725No Hit
CCGGGATCACGAAAGCTTACCCTCGG82350.11779619413156096No Hit
TGTGTTTTCCAGTATGGGTGACGGTC81380.11640867369066706No Hit
ATTACTCCAGTCAGCCAAACCACAGT81200.11615119567070736No Hit
GTACTCCAGTGTACTCCCAATGCACT81120.1160367609951697No Hit
CCAGCGACACAGCCCACGCTGATCGG79630.11390541516328112No Hit
GGAGCAAGTCAAACTCGTCCCGACTG79290.11341906779224614No Hit
CGAATGTAGTCCTCCTCCAGTAGCAG79050.1130757637656332No Hit
ACGAGCCTCACGCGGTGGTTCTGCTA78900.11286119874900014No Hit
GTAGGCCCAGTGACAGGTTTCAATAG78600.11243206871573398No Hit
GCTCTGTCATCATCCCACACCGATCT78590.11241776438129175No Hit
GGAATAAAGACTAGGCTGTCGAGAGC78020.11160241731808607No Hit
AGTCTCTCAGCTGGTACACGGCAGGG77480.11082998325820698No Hit
AAACCTGAGTCCAGGACATGATCGTT76890.10998602752611684No Hit
TCTGAGACACAACGCCCGTGCCTCGC76710.10972854950615715No Hit
CGTCTACTCTGTCTCGAACTAATATG75700.1082838117274944No Hit
GACCTGGAGTATTGGACTGATAATGG75660.1082265943897256No Hit
CGGACTGCAGGAATCGGAGTGTACCG74950.10721098664432903No Hit
AAATGCCGTCGGGTCTTTCGTTGGCA74840.10705363896546474No Hit
GACCATGGCCGTGGGGATGAGGTCCA74660.10679616094550505No Hit
GCACTCTAGACACGACAATGTCACGG74220.10616677023004802No Hit
GCTTGAATCAACCATGCGGGCCCCAA74180.1061095528922792No Hit
ATCTACTCACAGACTTGCGCCGCGTC74000.10585207487231951No Hit
AAACCTGAGTCCAGGATGCGCCTCGT73780.105537379514591No Hit
CGTTCTGAGAATTGTGTCTAATGGGT72930.10432151108700355No Hit
TGAGCATGTTACGGAGCCGGTAGGGC72900.10427859808367693No Hit
CAACTAGTCAAACGGGATTTAGGTAC72820.10416416340813929No Hit
GGGCATCGTCCGAACCCCCCTAGGCT72350.10349185968935565No Hit
GTATCTTCAGGCTCACTATCAACCAA70740.10118886184416057No Hit
CTCACACGTAGCGATGGATGTAAGCG70370.10065960146979898No Hit
TGCCAAACAGTCTTCCTTCATTCTGC70180.10038781911539706No Hit
AACTCCCTCTATCCTAACAAGCGGGG70150.10034490611207046No Hit
GGACATTTCAACGAAAGGGACCTGGG70110.10028768877430162No Hit
GACCTGGTCTCGAACGACTCTTTAGT70050.1002018627676484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)