FastQCFastQC Report
Thu 31 Oct 2024
H23Y2DMX2_n01_CITE-seq_test_BCells_GD1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23Y2DMX2_n01_CITE-seq_test_BCells_GD1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40022848
Sequences flagged as poor quality0
Sequence length26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTAGAGTTGTCGTTATTTGATGT1157580.28922979194284226No Hit
CAACCAACATGTCGATCACACTTCCA1148810.2870385435839049No Hit
TGACTAGTCTATCCTAAGCACATCAA1049050.26211278117939035No Hit
CCAATCCGTGACCAAGAAGGCAGTCA1024310.25593131203456587No Hit
TGTATTCAGAGCTATAACCCAAGGTG1003930.25083922063717207No Hit
TGACAACGTCTTCTCGACCTGGGCGG992560.2479983433462806No Hit
AGCGGTCTCAAGATCCACAAATGCTT967520.24174191701699987No Hit
CCCAGTTCAAACTGCTTAGCGACTGG929710.23229481320269862No Hit
AAGGAGCAGATATGCAGGGAGGCGGT908480.22699034311601213No Hit
CACATTTGTTATCGGCATTGTTTCCT870900.21760070647646065No Hit
CACACAACAATCCGATAGGCCGGATT789270.19720485658591813No Hit
CATCAAGCACTTGGATGCGCAGGAGA786510.1965152504889207No Hit
AAGGTTCTCATTTGGGTCATCGAATC754230.18844985744142947No Hit
CACACAACAATCCGATATTCGTGGCC748830.18710062812121717No Hit
TCCACACCAAGTCTGTGATTAATTGT741380.18523919137388725No Hit
ATGAGGGAGCGCTTATTTTACACGCA729900.1823708297820285No Hit
ACGGCCAAGGACACCAATACTTCGGG719150.17968486400567996No Hit
CAACCAATCAGTTCGATCGTACCGTT716770.17909020367566045No Hit
GAATGAACATCCAACAGCAATGTCCT713290.1782207003359681No Hit
TACCTTATCTCCAACCCGAGATGGCT710120.1774286527535472No Hit
CACACAACAATCCGATCCCTGGCCGC709400.1772487555108522No Hit
TACCTTATCTCCAACCGGGCCCGCTA707390.1767465423749954No Hit
CTGTGCTTCGGTGTCGCATCAATTCA705620.1763042949867036No Hit
TACGGTATCCGAGCCACCTGTAACAG692110.17292872311335764No Hit
TGGTTAGTCAGCAACTCTTCGACCAG690780.17259641292893502No Hit
TGATTTCTCGGCGCTAGTTGTTATGA689040.1721616612590888No Hit
GCTGGGTCACACGCTGAGCACGTACA686810.17160447952129743No Hit
TGATTTCTCGGCGCTATGTTTGTCAG673280.16822391050232108No Hit
GCTGGGTCACACGCTGGCCGCCGGTC670280.16747433865775868No Hit
AGCGGTCCAAGTAATGCACGGGTCTA670000.16740437861893287No Hit
AAGGTTCTCATTTGGGTGAATCTGGA669530.1672869456966181No Hit
ACCAGTAGTCCGCTGACCTTGTCCGG664260.16597019782300348No Hit
TGGCGCATCGGCATCGCTGCGGGTTC660530.16503823016293093No Hit
ATCACGAAGGCAGGTTCCCCACGACT657210.1642087039882819No Hit
CCCAATCGTCTCCATCGTCATACCTG649400.1622573186196045No Hit
GTGCATAGTTCACCTCCAATCGTGTA644720.16108798654208717No Hit
TGCGCAGAGCAGGCTATAGCGTAGTG643540.1607931549498926No Hit
CAACCTAGTATGACAGACTGCCTGTT641130.16019099890142752No Hit
ATGAGGGAGCGCTTATCAGCTGGGGA639930.15989117016360255No Hit
CACACAACAATCCGATAACAGGCACC635910.15888674389188895No Hit
GATCGATTCGTGACATTCCACTCGGG633010.15816215777547865No Hit
GTGCAGCGTTCCAACAGCCTCGGCCG632430.15801724055219657No Hit
AGATCTGAGCTCCTCTGATCACATTA630710.1575874860279808No Hit
CAACCAACATGTCGATACGACACAGT630420.1575150274163398No Hit
AGTAGTCCAAGACACGGCTCCCGTAT626790.1566080454844193No Hit
AGCTCTCGTTGCCTCTACGGCTGCCG621380.1552563175913918No Hit
ATCATGGAGAAGCCCAATCGCGACGG615910.15388959826147305No Hit
GGCGACTCACTGTCGGGTCTTAGGAT612590.153060072086824No Hit
CCACGGATCTAACTCTCAGCCCGCGT601670.15033163057261692No Hit
AGCGGTCCAAGTAATGGAGCGTGCCG594650.14857763245634095No Hit
CCCAGTTCAAACTGCTTTCGGGTGGC590340.1475007475729863No Hit
CGGGTCACACTGCCAGGCAGCGGGTT589190.1472134116992374No Hit
TGACAACGTCTTCTCGAGGTGCGCGA587000.14666622425270687No Hit
TTGACTTGTATAGGTATCATTGCCTG584940.146151518252774No Hit
CAACCAATCAGTTCGACGGGAGGCTG583500.14579172376738406No Hit
GAATGAACATCCAACAGAGCTCGTTG579500.1447922946413009No Hit
GATCGATTCGTGACATCATTCGATTG575120.14369791974823978No Hit
GCAGTTACATCCGCGATATGCACATC574240.14347804534050151No Hit
TGAGAGGCATTACGACTCGGGATCTA573260.14323318520461112No Hit
GCTGGGTCACACGCTGCAGATTGGGC573150.14320570090364382No Hit
CCCATACCAATAGCGGGCGCCCTGGG570450.1425310862435377No Hit
AGTGGGAGTTAAGGGCTGATTGGTGG567490.14179150869023616No Hit
CCAATCCGTGACCAAGGATGGCAGCT563680.1408395524476419No Hit
GATCGATTCGTGACATGCGACGGTTC563020.14067464664183818No Hit
ATGAGGGAGCGCTTATTAGTCAAGGG562760.14060968374864277No Hit
ACGGCCATCAGTTCGAGGGCTCCGTG561050.14018242779724222No Hit
TTGACTTGTATAGGTACACCTGGCAT548130.13695427171999353No Hit
CACACAACAATCCGATTGAGGGGGTC545420.13627715848707217No Hit
GGGACCTCAATTCCTTACCCCACTCA542810.1356250309823029No Hit
ATTATCCTCCTGCTTGCAGCAATCGC533610.13332634399231158No Hit
TTGACTTGTATAGGTACGGGCTCCGC530610.1325767721477492No Hit
AAGCCGCAGTCCTCCTACGGCACGGG530090.1324468463613584No Hit
ATGAGGGAGCGCTTATCGGAGTAGGG529900.13239937347786943No Hit
AAAGATGAGAACTGTACTTCAAACTA522840.13063538107033262No Hit
GTATCTTGTACAGACGAGGGGCTTAG520400.13002572930342188No Hit
GCATACAAGTGTACCTGCCTCACCTT519250.12973839342967297No Hit
CCACGGATCTAACTCTCAAAAGCCAA515940.12891136582783913No Hit
CACACAACAATCCGATAGGAACGGAC515900.1289013715365783No Hit
GACTACAGTGTATGAGGGGCCAGAGT515680.12884640293464375No Hit
AGCGGTCCAAGTAATGGAACGGGTAG512180.12797190244932094No Hit
CACCAGGCATCACAACATGCCCGCAG510960.12766707656586557No Hit
CGGACGTCATGTCCTCGAAACTTGGG508710.1271048976824438No Hit
CCTACCAAGTTTGCGTACCCCTCTAG506650.12659019168251096No Hit
ACTTGTTGTATGGTTCCGGCGGATGT503530.12581063696416608No Hit
GTGCAGCGTTCCAACATCAACCTTCT500840.12513852087687513No Hit
GCGACCATCCAGGGCTTGGAATTCAC496600.12407912600322696No Hit
TTGGCAAAGTTCGATCCCCCATTGGC496540.12406413456633571No Hit
GGATGTTGTTCCACGGACATTACTGA496000.12392921163431449No Hit
GGGACCTCAATTCCTTGATTTAACAT489830.12238759220733117No Hit
GTCAAGTCACTGTGTAATGGGAGCGG489760.12237010219762472No Hit
TTGACTTGTATAGGTATCAGGGATGC487020.12168549324625773No Hit
GTGCATAGTTCACCTCATTCACAACT486470.1215480717414213No Hit
ACCAGTAGTCCGCTGAGCGGGATGCA485370.12127322873174842No Hit
TTGGCAAGTTGGAGGTGCATTGCAGT482650.12059361692601187No Hit
CAACCAATCAGTTCGATCCCCTTGCT480900.12015636668335047No Hit
GCAGTTACATCCGCGACAGGGAGTAG480600.12008140949889425No Hit
GTAACGTTCAGTTGACCCGTGGGGCT477980.11942678342130975No Hit
TCGGGACGTCTAGTGTGCTAACGCCT474340.11851730291657404No Hit
GCATGTAAGATCTGCTCCTCGAGGTT469660.11734797083905674No Hit
GCGCAACTCCTCCTAGTCATTATTAG468210.11698567778085159No Hit
GGAGCAAAGTGTGGCACACCCTTCCG467530.11681577482941743No Hit
TGGACGCGTTTGTGTGCTAGGTAGGA464190.11598125150913798No Hit
CATCAGAAGTGACTCTTCTCTAAGGG463100.1157089070722803No Hit
CGCGTTTCACGGATAGTCATGAGGGG462370.11552651125677015No Hit
CAGAATCAGTACGATAGCATGTAGGT461590.1153316225771839No Hit
AGACGTTCAACTGCGCACACAATCTA453890.11340772150947379No Hit
GTAACGTAGGGTCGATTATGCAGGGT452670.11310289562601841No Hit
AGACGTTCAACTGCGCCTAACTTGGG452220.11299045984933405No Hit
GCGACCATCCAGGGCTAATCCGGCGC451250.1127480982862589No Hit
CACACCTAGTATGACACTGCCTGCCC441650.11034946838365926No Hit
TTGTAGGGTCTCGTTCATAGGCACAC439600.10983726095654162No Hit
CAGAATCAGTACGATATACCTTAGTA437680.1093575349760217No Hit
CGCGGTACAGTCGTGCGATGCCCTTG435130.10872039890814367No Hit
GGAGCAAAGTGTGGCAACCGCATAAC433220.10824317150043895No Hit
ATAACGCAGTACGACGGATGTGTTGA433000.10818820289850437No Hit
GTGCGGTAGTGCAAGCCAGACCCGTT428620.1070938280054433No Hit
TACGGTATCCGAGCCATGCTTAGCAG426180.10648417623853254No Hit
GTGTTAGAGGGAACGGCTCACCGCTG423960.10592949307355638No Hit
AGCGTATTCCTAGGGCGTTCCTAGCT423130.10572211152989414No Hit
CCAATCCGTGACCAAGGGAATGCGGG423050.10570212294737245No Hit
TACCTTATCTCCAACCGCGATGGGCT422680.10560967575320977No Hit
AAATGCCTCGTCCAGGTTGAGACCTC421480.1053098470153848No Hit
CAAGTTGCAAGTACCTACTCGTTACG418190.10448781655918139No Hit
AATCGGTAGGGTCGATCGACTGGTGC417840.10440036651064911No Hit
GCTGCTTAGGATGCGTAGCGGAGGCT416170.1039831048505094No Hit
ACCAGTAGTCCGCTGATCTAGAGGTG416110.10396811341361813No Hit
CAACCAATCAGTTCGAATTATCGCGC415650.10385317906411859No Hit
ACGGCCAAGGACACCAAGCTGCACCG415580.10383568905441211No Hit
CAAGTTGCAAGTACCTCACCAGCCGC413820.10339594023893552No Hit
AGAGTGGAGATGTGTAAAGCGTCATT413770.10338344737485948No Hit
CTAATGGAGTAGGCCAACGATACCTC413650.10335346450107699No Hit
TGGTTAGTCAGCAACTAGTTTTGTAA410660.1026063912293298No Hit
TACGGTATCCGAGCCAACCAACTCCT410210.10249395545264546No Hit
ATAACGCAGTACGACGGCTGTGGTCA410160.10248146258856941No Hit
ACTGAACTCCCGGATGTGCGAACGAA409430.10229906677305922No Hit
TGGTTAGTCAGCAACTGTAGCACGGC408880.1021616452682228No Hit
AGCGTATTCCTAGGGCCAGTACGTCG408710.10211916953036426No Hit
ATTATCCTCCTGCTTGAGCTACTGCT408230.10199923803523427No Hit
CGAGCACGTTAAGACATCCCCATTCA406970.10168441786051809No Hit
ACATGGTAGTTGTCGTCTGTTGCGCG405710.10136959768580188No Hit
TTGGAACTCCGCGCAAGGAGCGTTCG402810.10064501156939157No Hit
GAATGAACATCCAACATCGATTACCT402300.10051758435581595No Hit
GGTGCGTTCTGCTGTCTGTGCCTCCA401380.10028771565681682No Hit
CGGGTCACACTGCCAGGAGTGGCGAG401340.10027772136555599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)