FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_8.3410000000970d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_8.3410000000970d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530783
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20770.39130868923835166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.33535361908727296No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13080.24642838975626574No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA12220.23022591153070088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9770.18406768867880094No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8980.1691840168204332No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8900.16767680954363648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.16353198953244547No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6750.1271706139797243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCC903.947207E-1027.49907914
ATAATAC608.182822E-626.249123
TCCGAGC957.403287E-1026.0517613
CGAGCCC957.403287E-1026.0517615
TTAAATC1159.094947E-1225.4339333
ATGCCGT1001.3424142E-924.74917244
CGGGGGG651.5221391E-524.22995817
CACGAGA1152.6375346E-1023.47747421
GTATTAA702.668862E-522.533211
CGTATGC1104.0417945E-922.49924741
TATGCCG1104.0417945E-922.49924743
CCCACGA1156.7502697E-921.52101719
GACCTGA1156.7502697E-921.52101726
AGCCCAC1201.1023076E-820.6243117
ATAATAG550.003934552420.4538613
ATCGTGC550.003934552420.4538618
CTCGTAT1251.7626007E-819.79933539
ATCCTAT807.468045E-519.7165581
TGTCCCC1051.443037E-619.28506910
TGATTAA1302.7648639E-819.03782530
Produced by FastQC (version 0.11.2)