FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_5.341000000096d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_5.341000000096d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217595
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA24351.1190514487924814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23231.0675796778418623No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18100.8318205841126864No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA14980.6884349364645328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.6475332613341299No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12170.5592959397044969No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11040.5073645993703899No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.4738160343757899No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7680.35294928651853213No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7340.33732392747995127No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5430.2495461752338059No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.24632918954939223No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA5240.24081435694754014No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4860.22335072037500864No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4570.21002320825386614No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC4450.20450837565201405No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3330.1530366047013948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2990.13741124566281393No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2960.13603253751235092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT2850.13097727429398653No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.1286794273765482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT2740.12592201107562215No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.11764976217284405No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.11719019278935638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2520.11581148463889336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.10799880511960293No Hit
GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2210.10156483375077553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT2180.10018612560031251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG556.002665E-1140.90762736
ATGCCGT556.002665E-1140.90762744
GTATGCC659.094947E-1238.07555842
ATCTCGT601.546141E-1037.4986637
TACTTAT601.546141E-1037.4986632
AGACCTC601.546141E-1037.4986625
GACCTCT601.546141E-1037.4986626
TGCCGTC601.546141E-1037.4986645
ACGTGTT250.002103733135.9987147
GTCAACT250.002103733135.99871412
CGTATGC653.6379788E-1034.61414341
ACTTATC653.6379788E-1034.61414333
TATGCCG653.6379788E-1034.61414343
CTTATCT653.6379788E-1034.61414334
TCGTATG653.6379788E-1034.61414340
AGTCCAA352.813505E-432.1417122
CTACTTA708.058123E-1032.1417131
CACGAGA708.058123E-1032.1417121
CGTTCTT352.813505E-432.1417110
ACCTCTA708.058123E-1032.1417127
Produced by FastQC (version 0.11.2)