FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_4.341000000096c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_4.341000000096c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057972
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.026.186521
GGTATCA4700.023.4867521
GCGGGGC550.003936980420.45396611
CGCGGGG600.006511227718.7494710
CGGGGCC600.006511227718.7494717
CCTAGTC851.2045876E-418.5288873
GCCCACG1002.046884E-517.9994918
TATCAAC16800.017.8119952
AACGCAG16500.017.726776
TCAACGC16600.017.6199824
GTCCTAT1305.706388E-717.3293131
CAACGCA16750.017.3278685
CGCAGAG17100.017.2363538
TAGACCG1358.583356E-716.6661955
ACGCAGA18050.016.3291787
ATCAACG18550.016.1316173
GACCTAG850.002543704415.9021951
CAGAGTA18400.015.89628810
GCAGAGT18550.015.7677469
CACGTAC1004.1675477E-415.7495557
Produced by FastQC (version 0.11.2)