FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_3.341000000096bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_3.341000000096bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269724
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.19019442096365174No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5040.18685767673621925No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4730.17536444661950734No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG4590.17017395559905682No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC4400.1631297177855882No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC4310.15979297355815575No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.14533374857261497No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770.13977250819356082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.11678604796013703No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT3090.11456155180851538No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA3010.11159555693968651No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT2930.10862956207085762No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2770.10269757233319986No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA2750.10195607361599265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC301.13793794E-437.49814641
CTCGTAT301.13793794E-437.49814639
TCGTATG301.13793794E-437.49814640
TGGTACG401.556685E-533.74833310
GCCCACG352.8155104E-432.14126618
TATGCCG352.8155104E-432.14126643
ATGCCGT352.8155104E-432.14126644
CACCCAT352.8155104E-432.1412667
TAGACTG300.005141600529.9985165
GTATGCC406.155592E-428.12361142
GGAGTCC507.14085E-526.9986638
CACGAGA450.001224474524.99876221
TCTCGTA450.001224474524.99876238
CGAGACA450.001224474524.99876223
TAAGAAC450.001224474524.9987623
ACGTTAG551.3443845E-424.5989651
GCGGGGG551.3648724E-424.5442425
TGCCTAC702.6481413E-522.5490511
CCCACGA500.00226080222.49888619
ACGAGAC500.00226080222.49888622
Produced by FastQC (version 0.11.2)