FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_2.341000000096af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_2.341000000096af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262049
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC2690.10265255734614519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC453.8198777E-1044.99885641
TATGCCG552.732122E-936.81724543
GCCCACG606.3937478E-933.74913818
GTATGCC606.3937478E-933.74913842
CGCGCGC551.12231646E-732.7264410
TCGTATG551.12231646E-732.7264440
TAGACAA651.3951649E-831.15305330
CGAGACA651.3951649E-831.15305323
CGGGGCA1100.030.68103817
GCTTATA300.005112005430.0336251
TCTCGTA602.4074689E-729.99923338
TCGCGCG602.4074689E-729.9992339
ATAGACA702.869092E-828.92783529
CATAGAC702.869092E-828.92783528
ATGCCGT702.869092E-828.92783544
TCTATAC406.154259E-428.1242833
GGGGCAA1053.6379788E-1227.85643418
TACCCGG1053.6379788E-1227.8564346
AGCCAGG1150.027.39060626
CCCACGA755.6057615E-826.99931319
Produced by FastQC (version 0.11.2)