FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_19.341000000097c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_19.341000000097c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704755
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG300.005098960430.05631
GGTATCA3850.029.8611261
GTATCAA12100.029.06271
CGGCAGG551.367864E-424.5444135
ATTCGGC602.466906E-422.49904332
TATGCCG500.002264307622.49904343
CCTTATA654.2372072E-420.7683472
TATCAAC17050.020.4536742
AACGCAG17050.020.1897566
CAACGCA17300.019.7679445
ACGCAGA17450.019.598027
CGCAGAG17450.019.598028
ATCAACG17600.019.4309923
TCAACGC17600.019.4309924
TCGGCAG706.981799E-419.28489334
TTCGGCA706.981799E-419.28489333
TAGACGG600.00650965418.7492035
GCGCTAT600.00650965418.7492036
AGAGTAC19000.018.1176511
GCAGAGT18900.018.094479
Produced by FastQC (version 0.11.2)