FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_18.341000000097ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_18.341000000097ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758748
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG551.3679435E-424.54455818
GGTATCA3850.023.9997021
GTATCAA8700.023.572461
CTAGTAC802.9734874E-622.4991783
TAGACAG1201.1041266E-820.6242435
GACTGCG908.247791E-619.9992687
CTATACT807.5607975E-519.686784
GTCTATG1555.2204996E-1018.9014191
GGGGGTC600.006509881518.74931118
CTAGCAC600.006509881518.7493113
TAAGGTG851.2040944E-418.5287325
TATCAAC10700.018.2937222
AACTAGT750.001097806818.0290451
GTCCTAT1153.3929446E-617.637111
ACCGTGC901.8651449E-417.499368
CCCTTAA1053.0674186E-517.1705191
GTATTAT1459.478572E-817.0965081
GTGTATA1205.089705E-616.902231
ACTAGTA800.0017105416.8743822
CGGGGGT952.8185578E-416.5783417
Produced by FastQC (version 0.11.2)