FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_16.34100000009791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_16.34100000009791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977479
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG1650.027.2717959
GGGGCTA1600.026.7178436
CGCGCGC1800.024.99914710
GCGCGCT2400.024.37416811
GGTATCA4300.023.5818981
GTCGCGC2050.021.9504728
GTATCAA11550.021.4607731
GGCGGGG855.0324215E-621.1757524
CGCGCTA754.602938E-520.99928512
GTCTAGG654.1948713E-420.8004441
CGTATGC1109.983887E-820.45384841
CGCGCTT2550.020.29342712
GGCTAGT1900.020.13089238
GTCCGGG1351.9645086E-919.99931731
GGTCGCG2300.019.564557
GGGTCCG1503.43789E-1019.49933429
GTCTACG2000.019.1537421
CGGCCTA951.3158895E-518.94672212
GCGGGGG951.3158895E-518.94672225
AGCGCCA2150.018.83656720
Produced by FastQC (version 0.11.2)