FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_15.34100000009784.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_15.34100000009784.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449505
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG6270.13948676877899022No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5280.11746254212967597No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4550.10122245581250487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG1400.032.1413469
CGCATGA300.005145144629.9985942
CGTAGGA453.4872843E-529.99858927
ATCTTTA453.4872843E-529.99858931
GTCGCGC1650.028.6350178
TACCATT450.001225728524.9988257
CACATTC450.001225728524.9988257
ACATTCG450.001225728524.9988258
GCACGGT751.6945069E-623.99887314
CATAATC751.6945069E-623.9988733
GCGCCAT1950.023.07583821
CGCGCGC2250.022.9989210
AATATGT500.002263098722.49894328
TCAATGC602.465051E-422.49894344
TAGGGAG802.9692837E-622.4989435
CGCACGG802.9692837E-622.49894313
TAGGAGT500.002263098722.49894329
GGGATAG500.002263098722.4989437
TACCGAT500.002263098722.4989437
TAAGTAT1358.367351E-1121.66564810
Produced by FastQC (version 0.11.2)