##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb3_3_14.34100000009777.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 169481 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.954738289247764 38.0 38.0 38.0 32.0 38.0 2 36.95552893834707 38.0 38.0 38.0 32.0 38.0 3 36.88330255308855 38.0 38.0 38.0 32.0 38.0 4 36.95929337211841 38.0 38.0 38.0 32.0 38.0 5 36.94065411462052 38.0 38.0 38.0 32.0 38.0 6 38.93811695706303 40.0 38.0 40.0 38.0 40.0 7 38.84587062856603 40.0 38.0 40.0 38.0 40.0 8 38.8378992335424 40.0 38.0 40.0 38.0 40.0 9 38.81571975619686 40.0 38.0 40.0 38.0 40.0 10 38.77848254376597 40.0 38.0 40.0 38.0 40.0 11 38.826305013541344 40.0 38.0 40.0 38.0 40.0 12 38.80613756114255 40.0 38.0 40.0 38.0 40.0 13 38.812026126822474 40.0 38.0 40.0 38.0 40.0 14 38.84519798679498 40.0 38.0 40.0 38.0 40.0 15 38.773325623521224 40.0 38.0 40.0 38.0 40.0 16 38.81118827479187 40.0 38.0 40.0 38.0 40.0 17 38.82521344575498 40.0 38.0 40.0 38.0 40.0 18 38.820746868380525 40.0 38.0 40.0 38.0 40.0 19 38.84586472819962 40.0 38.0 40.0 38.0 40.0 20 38.804373351585134 40.0 38.0 40.0 38.0 40.0 21 38.82858845534307 40.0 38.0 40.0 38.0 40.0 22 38.78349785521681 40.0 38.0 40.0 38.0 40.0 23 38.796213144836294 40.0 38.0 40.0 38.0 40.0 24 38.84615384615385 40.0 38.0 40.0 38.0 40.0 25 38.83555088771013 40.0 38.0 40.0 38.0 40.0 26 38.80024899546262 40.0 38.0 40.0 38.0 40.0 27 38.74947634248087 40.0 38.0 40.0 38.0 40.0 28 38.693422861559704 40.0 38.0 40.0 38.0 40.0 29 38.54495784188198 40.0 38.0 40.0 38.0 40.0 30 38.52364571839911 40.0 38.0 40.0 38.0 40.0 31 38.40042836660157 40.0 38.0 40.0 38.0 40.0 32 38.24257586396115 40.0 38.0 40.0 38.0 40.0 33 38.02580230232297 40.0 38.0 40.0 38.0 40.0 34 37.77452339790301 40.0 38.0 40.0 32.0 40.0 35 37.575551241732114 40.0 38.0 40.0 32.0 40.0 36 37.33268035945032 40.0 38.0 40.0 32.0 40.0 37 37.136351567432335 40.0 38.0 40.0 32.0 40.0 38 36.96784300305049 40.0 38.0 40.0 27.0 40.0 39 36.74569420761029 40.0 38.0 40.0 27.0 40.0 40 36.48408966196801 40.0 38.0 40.0 27.0 40.0 41 36.23970238551814 40.0 38.0 40.0 27.0 40.0 42 36.00272006891628 40.0 38.0 40.0 13.0 40.0 43 35.677786890565905 40.0 38.0 40.0 13.0 40.0 44 35.345655265191965 40.0 38.0 40.0 13.0 40.0 45 35.01238486910037 40.0 38.0 40.0 13.0 40.0 46 34.62066544332403 40.0 38.0 40.0 13.0 40.0 47 34.13495318059252 40.0 32.0 40.0 13.0 40.0 48 33.70222620824753 40.0 32.0 40.0 13.0 40.0 49 33.34028593175636 40.0 27.0 40.0 13.0 40.0 50 32.9614647069583 40.0 27.0 40.0 13.0 40.0 51 32.365716510995334 38.0 27.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.1624377071832228 2201 2 0.3832479996411635 2201 3 0.39829176931608856 2201 4 0.37010723889215313 2201 5 -0.02205874140365438 2201 6 0.3250769759493508 2201 7 0.4191921920892341 2201 8 0.3516202317972983 2201 9 0.5868214407383476 2201 10 0.6931989663463938 2201 11 0.5823604826313797 2201 12 0.2858836944411678 2201 13 0.23651157816952662 2201 14 0.08541301626575404 2201 15 0.5563430210971916 2201 16 0.2661915474753016 2201 17 0.4689027675314321 2201 18 0.3007458817308759 2201 19 0.560671381183333 2201 20 0.48897961905555576 2201 21 0.4018504367021265 2201 22 0.6102913980535973 2201 23 0.2756871236040652 2201 24 0.14724477110035394 2201 25 0.28938324640856905 2201 26 0.19885074844462736 2201 27 0.46306820752667477 2201 28 0.5001542904657299 2201 29 0.44838388697433373 2201 30 0.29035631378669535 2201 31 0.7142810765014289 2201 32 0.71708586222816 2201 33 0.8156117082624021 2201 34 0.9927224068094134 2201 35 1.2199793691771248 2201 36 1.3371436741533103 2201 37 1.6645642116637092 2201 38 1.76168436968711 2201 39 1.4471462995549658 2201 40 1.5962581264003077 2201 41 1.5741718576978343 2201 42 1.5948781097798417 2201 43 1.7666334828592767 2201 44 1.5342263682927069 2201 45 1.2077355672975187 2201 46 0.9863791647212423 2201 47 1.1586467238073297 2201 48 0.8831347919839772 2201 49 1.1078510539982318 2201 50 0.8268252667416007 2201 51 1.1447794001156097 1101 1 0.2982574120097041 1101 2 0.12592849425624308 1101 3 -0.012916846559463124 1101 4 0.14637369282115031 1101 5 0.0762260611491925 1101 6 0.1614551171539773 1101 7 -0.0050599020471864264 1101 8 -0.11954051810297983 1101 9 -0.1511522329752566 1101 10 0.10672110435995563 1101 11 -0.18868060233072725 1101 12 0.04922633504747154 1101 13 -0.11586177643756912 1101 14 -0.21773816043947392 1101 15 0.07126125005610362 1101 16 0.25442449522161326 1101 17 0.08039060159605071 1101 18 -0.16199843418456084 1101 19 -0.24008649675842975 1101 20 0.024879100507490648 1101 21 -0.23572435388422974 1101 22 -0.0015953191382678256 1101 23 0.13611791738149037 1101 24 0.16898390153513532 1101 25 -0.040014440069292334 1101 26 0.04041437030342365 1101 27 0.414643795879293 1101 28 -0.09948671471975246 1101 29 -0.002074828621992708 1101 30 -0.1691570487980627 1101 31 0.17618773784965214 1101 32 0.38689040949580544 1101 33 -0.028624430548923385 1101 34 0.05981454881180781 1101 35 0.17550390048945275 1101 36 -0.0825212640198032 1101 37 -0.06810431645754988 1101 38 0.28114986629660876 1101 39 0.3238515249239313 1101 40 0.5608373047010673 1101 41 0.4482843427397114 1101 42 0.3889347511839105 1101 43 -0.01812918168001687 1101 44 0.40159772848417674 1101 45 -0.21747384634428357 1101 46 -0.6007369102687861 1101 47 -0.7764549913901178 1101 48 -0.5315441070985898 1101 49 0.11945855300102437 1101 50 0.43691700986086346 1101 51 0.06707696692853915 2202 1 0.09021011986630612 2202 2 0.3284837955529625 2202 3 0.31942487731544134 2202 4 0.20907392165410243 2202 5 0.4450666408593378 2202 6 0.333728191143301 2202 7 0.4982200369306895 2202 8 0.3868286657261564 2202 9 0.5388172559644886 2202 10 0.23307503265896656 2202 11 0.2953010910331173 2202 12 0.04819658676371574 2202 13 0.3116363166211613 2202 14 0.5206294978366515 2202 15 0.3598196024588489 2202 16 0.4046914669988837 2202 17 0.6320687099103139 2202 18 0.3218307038779855 2202 19 0.35316293115918995 2202 20 0.40950915390184406 2202 21 0.5588599329197308 2202 22 0.39123458170078607 2202 23 0.3988562850397628 2202 24 0.46742020969684717 2202 25 0.25224337838989896 2202 26 0.2191710445978572 2202 27 0.583179264986839 2202 28 0.4237016910773477 2202 29 0.16985499593941 2202 30 0.12336774143839335 2202 31 0.07626401550029982 2202 32 0.46290108836689825 2202 33 0.24406817051896468 2202 34 0.6207603916798448 2202 35 0.4684100789791543 2202 36 0.3637411310984291 2202 37 0.4112244402167491 2202 38 0.055825686281352205 2202 39 0.46996136474086825 2202 40 0.38665728944553734 2202 41 0.6788716806497064 2202 42 0.6651742630069464 2202 43 0.5235946932246378 2202 44 0.8518265614361198 2202 45 1.176309525127877 2202 46 0.7333210607457019 2202 47 1.2446357790141533 2202 48 0.8561751911470239 2202 49 0.47506496032367806 2202 50 0.572881922141562 2202 51 0.46250030788963414 1102 1 0.20179590250472046 1102 2 -0.2684061566001503 1102 3 0.12177165334642837 1102 4 -0.07504546772844378 1102 5 -0.1577472119545149 1102 6 -0.2677186076719238 1102 7 0.17297510642260505 1102 8 0.15870152970428109 1102 9 0.053797889366173024 1102 10 -0.3474102715105474 1102 11 -0.2651910484063862 1102 12 0.15795963073728814 1102 13 -0.5043240388128751 1102 14 -0.25095856322509036 1102 15 -0.491057590523603 1102 16 -0.04917046054725205 1102 17 -0.25680119211747865 1102 18 -0.15185350664832953 1102 19 0.15978175093590608 1102 20 -0.11731928082132725 1102 21 0.01283578163242538 1102 22 -0.34697457756099936 1102 23 -0.13207331167930647 1102 24 -0.4501578263022452 1102 25 -0.2470161340275112 1102 26 -0.4352490966643927 1102 27 -0.2875771715590645 1102 28 -0.10660133922568349 1102 29 0.1251978986507325 1102 30 0.30119868242723413 1102 31 -0.15545163726798705 1102 32 0.2541405506589953 1102 33 -0.03332987003697241 1102 34 0.1076594575262817 1102 35 0.14567141413522933 1102 36 0.4526566015928424 1102 37 0.5200191542746211 1102 38 0.9291453490746946 1102 39 0.8426544611181725 1102 40 0.8935495200219776 1102 41 0.2457434054161709 1102 42 0.26126676699418994 1102 43 -0.047378193537724655 1102 44 -0.021891825440164325 1102 45 0.016762931370756462 1102 46 0.2647063421309852 1102 47 1.074608437934181 1102 48 0.17754680199231387 1102 49 0.5197220576123556 1102 50 0.11007718302103342 1102 51 0.2507208592680783 2203 1 -0.09268461697580221 2203 2 0.24295747976348991 2203 3 0.2084599650347414 2203 4 0.19438093919796273 2203 5 -0.013266692473990815 2203 6 0.23899134903803798 2203 7 0.11686038780788266 2203 8 0.10020585870861254 2203 9 0.033992694560978975 2203 10 0.05653994493966508 2203 11 -0.07509364580898392 2203 12 0.0957843060619652 2203 13 0.2576889482001121 2203 14 0.20834879608226942 2203 15 0.021442409476392754 2203 16 0.3781563792795879 2203 17 0.2849195871032961 2203 18 0.43981316001833903 2203 19 0.18364538729954205 2203 20 0.035386346884301645 2203 21 0.08166695046359251 2203 22 0.2657959852095573 2203 23 0.14644400433800797 2203 24 0.08891143776702393 2203 25 0.15509425558288115 2203 26 0.20798683407154073 2203 27 -0.1650663490482458 2203 28 -0.13046497558931947 2203 29 0.1812585047113373 2203 30 0.10801686424541401 2203 31 -0.2700079143242604 2203 32 -0.18666031514187154 2203 33 -0.22808095228805314 2203 34 0.036988461855280264 2203 35 -0.0033004473366347042 2203 36 0.21856569250193303 2203 37 0.1342507560062174 2203 38 -0.4277269452976 2203 39 -0.3076702142065031 2203 40 -0.1784742895018283 2203 41 -0.005338845666081227 2203 42 -0.11070292997550979 2203 43 0.15034907918954588 2203 44 -0.1286558947042309 2203 45 -0.5921115275037039 2203 46 -0.587512272587631 2203 47 -0.530802817477074 2203 48 -1.1107107737652626 2203 49 -0.9863385484482521 2203 50 -0.9380829901391365 2203 51 -0.5861839026366802 1103 1 -0.3432222513844039 1103 2 -0.062150047118230134 1103 3 0.21302985750785552 1103 4 -0.219194329619242 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1213 31 -0.4208461067440865 1213 32 -0.21710249201262144 1213 33 -0.04852555870205322 1213 34 -0.29909025534588096 1213 35 -0.5437887855290526 1213 36 -0.4768424707633727 1213 37 -0.3107079417586007 1213 38 -0.45171868485056166 1213 39 -0.19127079729688035 1213 40 0.02377303800059849 1213 41 -0.306602208153933 1213 42 -0.026567847371040898 1213 43 0.33322079930616866 1213 44 0.32769736088371815 1213 45 -0.2837053078729923 1213 46 -0.2081269470288376 1213 47 -0.16331010610681318 1213 48 0.4725375255544719 1213 49 -0.3555319400906214 1213 50 -0.4420917364948238 1213 51 -0.15258331954630222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 4.0 15 13.0 16 28.0 17 15.0 18 16.0 19 13.0 20 17.0 21 38.0 22 80.0 23 134.0 24 297.0 25 414.0 26 692.0 27 1034.0 28 1473.0 29 2273.0 30 3205.0 31 4403.0 32 5370.0 33 6436.0 34 7524.0 35 8119.0 36 9877.0 37 13540.0 38 19245.0 39 85220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.519965960062166 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19.95149898808716 33.65273983514376 22.401331122662718 15 21.8590874493306 25.64535257639499 32.189448964780716 20.30611100949369 16 18.223281665791447 28.977879526318585 30.329063434839306 22.469775373050666 17 19.611637882712515 24.58269658545796 28.49109929726636 27.314566234563166 18 23.9478171594456 21.71393843557685 32.70927124574436 21.62897315923319 19 26.825425858945838 21.17110472560346 34.203244021453735 17.800225393996968 20 23.64394828918876 20.376325369805464 34.57732725202235 21.402399088983426 21 23.845150783863676 22.039638661560883 35.6470636826547 18.468146871920744 22 20.707925962202253 23.408523669319862 36.42119175600805 19.462358612469835 23 20.877856514889576 25.72736766953228 36.45777402776712 16.937001787811024 24 20.676654020214656 25.79935213976788 36.318525380426124 17.20546845959134 25 17.847428325299 27.889851959806705 37.099143856833514 17.16357585806079 26 17.8450681787339 28.35716097969684 36.63891527663868 17.158855564930583 27 17.056189189348657 28.233253285029004 36.509697252199366 18.200860273422983 28 16.40773892058697 29.196193083590494 34.88650645205067 19.50956154377187 29 17.282173222957145 30.288350906591297 34.04747434815702 18.382001522294534 30 16.989515048884535 29.887125990524012 34.66406263828984 18.459296322301615 31 18.468736908562022 28.559543547654307 33.65628005499141 19.315439488792254 32 19.50779143384804 28.29756727892802 33.25682524884795 18.93781603837598 33 17.742991839793252 29.87709536762233 32.67268897398529 19.707223818599136 34 17.489866120686095 31.001115169252007 32.08560251591624 19.423416194145656 35 17.784884441323808 32.325747428915335 32.755294103763845 17.134074025997016 36 18.01145851157357 32.82019813430414 32.01243797239808 17.155905381724203 37 17.232020108448733 33.07745410990022 32.33046772204554 17.360058059605503 38 17.15649541836548 33.18779096181873 32.33813819838212 17.31757542143367 39 17.055599152707384 33.414955068709766 31.64838536473115 17.881060413851703 40 16.87150772062945 34.14306028404364 31.63422448534054 17.35120750998637 41 17.26506216036016 33.2532850290003 30.68662563945221 18.795027171187332 42 17.27509278326184 33.71528371911896 29.724275877531998 19.285347620087208 43 16.519845882429298 33.46333807329435 29.37438414925567 20.642431895020678 44 16.160513567892565 32.755294103763845 29.25814693092441 21.82604539741918 45 16.915760468725107 32.194169257910914 29.013871761436384 21.87619851192759 46 17.20546845959134 31.284332757064213 28.453336952224735 23.05686183111971 47 17.095721644314114 30.678955163115628 27.79131584071371 24.43400735185655 48 17.268012343566536 30.55150724860014 27.123394362789927 25.0570860450434 49 17.698739091697593 29.628099905004103 27.00597707117612 25.667183932122185 50 17.302824505401784 29.445778582849996 27.660327706350564 25.591069205397655 51 17.906431989426544 28.877573297301762 26.55046878411149 26.6655259291602 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 31.0 1 26.0 2 21.0 3 35.5 4 50.0 5 75.0 6 100.0 7 113.5 8 127.0 9 224.0 10 321.0 11 742.0 12 1163.0 13 2456.5 14 3750.0 15 5176.0 16 6602.0 17 7081.0 18 7560.0 19 7145.0 20 6730.0 21 6545.5 22 6361.0 23 6977.5 24 7594.0 25 7674.5 26 6191.0 27 4627.0 28 3822.5 29 3018.0 30 2980.0 31 2942.0 32 3895.5 33 4849.0 34 4816.0 35 4783.0 36 4079.5 37 3376.0 38 2672.5 39 1969.0 40 2266.5 41 2564.0 42 2913.5 43 3263.0 44 3841.5 45 4420.0 46 5496.5 47 6573.0 48 7852.5 49 9132.0 50 9110.0 51 9088.0 52 9275.5 53 9463.0 54 9450.5 55 9438.0 56 9639.5 57 9841.0 58 8597.5 59 7354.0 60 6882.5 61 6411.0 62 5819.0 63 5227.0 64 4376.0 65 3525.0 66 3099.5 67 2674.0 68 2381.5 69 2089.0 70 1847.0 71 1605.0 72 1377.0 73 1149.0 74 1001.0 75 663.0 76 473.0 77 403.0 78 333.0 79 245.5 80 158.0 81 115.0 82 72.0 83 54.0 84 36.0 85 19.0 86 2.0 87 5.0 88 8.0 89 4.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1581298198618134 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 169481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.306542217120224 #Duplication Level Percentage of deduplicated Percentage of total 1 82.54965308133592 44.004365669888614 2 10.057229110530466 10.722322163354855 3 2.7462050346418794 4.391720838480164 4 1.2545819583328026 2.675097045068196 5 0.7318983391613572 1.9507484857572532 6 0.39198986676778486 1.2537374628924152 7 0.33739549094893356 1.2589770887494731 8 0.2248398458955832 0.9588347789858969 9 0.2135027168691206 1.0242982431228278 >10 1.3307967789924218 13.3940073545366 >50 0.09066757300824636 3.2628667576067443 >100 0.057672714145674854 6.047507385113678 >500 0.00452249645661522 1.5332602031328957 >1k 0.00904499291323043 7.522256523310398 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 2291 1.3517739451619946 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2172 1.281559584850219 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1784 1.052625368035355 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 1415 0.8349018474047238 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1374 0.8107103451124315 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1346 0.7941893191567196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1063 0.6272089496757749 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 1036 0.6112779603613384 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 757 0.44665773744549536 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 756 0.4460677008042199 No Hit CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC 527 0.31094930995214803 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 504 0.2973784672028133 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 495 0.2920681374313345 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 482 0.28439766109475395 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 465 0.2743670381930718 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 437 0.2578460122373599 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 300 0.17701099238262696 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 300 0.17701099238262696 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 285 0.1681604427634956 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 285 0.1681604427634956 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 277 0.16344014963329223 No Hit TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 264 0.15576967329671174 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 235 0.13865861069972446 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 235 0.13865861069972446 No Hit GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 235 0.13865861069972446 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.1374785374171736 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 223 0.13157817100441938 No Hit GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA 211 0.1244977313091143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 208 0.12272762138528803 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT 201 0.11859736489636007 No Hit ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 199 0.11741729161380923 No Hit GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 194 0.1144671084074321 No Hit GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 193 0.11387707176615668 No Hit GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGTGGAGC 189 0.11151692520105498 No Hit ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA 184 0.10856674199467788 No Hit GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG 183 0.10797670535340244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 170 0.10030622901682194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 5.900366412754232E-4 17 0.0 0.0 5.900366412754232E-4 18 0.0 0.0 0.0017701099238262696 19 0.0 0.0 0.002360146565101693 20 0.0 0.0 0.004130256488927962 21 0.0 0.0 0.015930989314436428 22 0.0 0.0 0.02596161221611862 23 0.0 0.0 0.050743151149686395 24 0.0 0.0 0.06844425038794909 25 0.0 0.0 0.08083501985473297 26 0.0 0.0 0.10030622901682194 27 0.0 0.0 0.12803795115676683 28 0.0 0.0 0.24014491299909724 29 0.0 0.0 0.49445070538880465 30 0.0 0.0 0.8301815542745205 31 0.0 0.0 1.1151692520105498 32 0.0 0.0 1.363574677987503 33 0.0 0.0 1.5765779054879308 34 0.0 0.0 1.83324384444274 35 0.0 0.0 2.0851894902673456 36 0.0 0.0 2.2852119116597143 37 0.0 0.0 2.5938010750467604 38 0.0 0.0 2.985585404853641 39 0.0 0.0 3.50658775909984 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAACGC 20 7.0169347E-4 44.99911 3 GCCTGTG 30 1.1359511E-4 37.49926 7 GTCTAAC 25 0.0021022563 35.999294 29 TAGAATA 30 0.0051355213 29.999409 4 CGGTTCA 45 3.4739576E-5 29.999409 14 ACGGGTC 30 0.0051355213 29.999409 15 CCCACCC 30 0.0051355213 29.999409 43 TCCAGAC 30 0.0051355213 29.999409 3 GTATCTG 30 0.0051355213 29.999409 25 GTTCAAA 45 3.4739576E-5 29.999409 16 GGTTCAA 45 3.4739576E-5 29.999409 15 TAGACAA 85 2.0008883E-10 29.117075 30 GACAAAT 85 2.0008883E-10 29.117075 32 ATAGACA 85 2.0008883E-10 29.117075 29 AGACATA 85 2.0008883E-10 29.117075 25 AAATCTC 90 3.8926373E-10 27.499458 35 CTCGTAT 90 3.8926373E-10 27.499458 39 CACGAGA 90 3.8926373E-10 27.499458 21 TATGCCG 90 3.8926373E-10 27.499458 43 GAGACAT 90 3.8926373E-10 27.499458 24 >>END_MODULE