FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_13.3410000000975e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_13.3410000000975e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917721
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1251.8189894E-1225.19920718
TACCCGG2000.023.6242566
CGTATGC1405.456968E-1222.49929241
GCGTTAT1459.094947E-1221.72345432
CGGGGCA3600.021.24933217
GTATCAA15800.021.1051831
ACCTATA2350.021.0930461
TATGCCG1501.4551915E-1120.99933843
CGGGCTG654.2382345E-420.76857617
GGTATCA6450.020.2606221
TAATACC1800.019.999374
GGGGCAA2700.019.99936918
CGTCGGG2500.019.79937714
ATACCCG2500.019.7993775
AATGCGT1503.43789E-1019.49938629
AACGCAG17000.019.190576
CCCACGA1656.002665E-1119.0903119
ATGCGTT1656.002665E-1119.0903130
ATGCCGT1656.002665E-1119.0903144
TATCAAC17600.018.9198592
Produced by FastQC (version 0.11.2)