FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_12.34100000009741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_12.34100000009741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452778
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG8620.19038027465998789No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC8390.18530052255189078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7660.16917783107836512No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7100.15680973898908518No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC6590.14554594083634806No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6180.13649073055669667No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA5980.13207355481052527No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5420.11970546272124528No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5200.11484656940045673No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG4880.10777908820658248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.10777908820658248No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT4730.1044662063969539No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA4590.10137418337463391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG606.4173946E-933.7483443
CGTCGGG2100.028.9271514
TTAGGAC406.162094E-428.1236213
ACCTATA2300.027.450591
TACCCGG2300.027.3899576
GGGGCAA2250.026.99867418
ATACCCG2450.025.7130245
CCCACGA801.0513395E-725.31125819
CTAGGGT450.001225757124.9987744
ATAATAC551.3668697E-424.544253
AGGCCTC2350.023.93499630
GCCTCGA2350.023.93499632
CTCGTAT851.8892752E-723.8223639
GGGTCCG1800.023.74883529
CGGCAGG952.1093001E-823.68304635
ATTCGGC952.1093001E-823.68304632
GCGACAG1850.023.10697423
GGTCCGG1850.023.10697430
GGTGCGA1850.023.10697420
GCCAGGC2650.022.92340327
Produced by FastQC (version 0.11.2)