FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_11.34100000009734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_11.34100000009734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753937
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10210.13542245572242773No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC9270.12295457047472136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.11101723353542803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC751.6967897E-623.9992943
CGTCCCG2050.021.95057125
ATACCCG2150.019.8831355
TACACTG1603.8198777E-1119.686925
CGTCGGG2200.019.43124614
CCTACAC706.981805E-419.2851453
TCGGCAG1801.0913936E-1118.74944934
ACCTATA2300.018.6110971
TACCCGG2300.018.5864096
CGCCACG2550.018.52886620
AATTTTA2600.018.17254339
GTACACT1002.0456364E-517.999474
CGGTTCC2500.017.9994730
CGGCAGG2001.8189894E-1217.9994735
TAACCGG2001.8189894E-1217.9994744
GTCCCGG2500.017.9994726
AACGCAG21800.017.9581856
CGTGGCG1651.2278178E-917.72675139
GGCGGTT1651.2278178E-917.72675142
GGTTCCG2550.017.6465431
Produced by FastQC (version 0.11.2)