FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_10.34100000009727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_10.34100000009727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754332
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA300.005146891329.9991179
GCACTCG551.3678672E-424.54473122
GCCCACG751.6967915E-623.99929418
GTATCAA21800.023.7693461
ACGACCG500.002264281322.4993385
GTATTAA854.9722294E-621.2039571
CTCGGTC754.6009838E-520.99938225
AACGCAG26000.019.7301866
GCGGCAC706.9818075E-419.28514719
CGAGCCC951.3151863E-518.9468115
TATCAAC27650.018.7969132
CACTCGG600.00650957718.74944923
TAGGCCG600.00650957718.7494495
ACGCAGA27650.018.634177
CGCAGAG27600.018.5864098
GGTATCA10550.018.5785851
CGAGTCG851.2040163E-418.5288666
TGGCGTG1102.2545246E-618.4085487
CAGAGTA28550.018.12555910
CCGAGCC1002.0456382E-517.9994714
Produced by FastQC (version 0.11.2)