FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_3_1.34100000009685.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_3_1.34100000009685.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241430
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAC352.8146594E-432.141233
CATACAG300.005140572329.9984845
GTATCAA2750.026.2402781
GAGTGCT450.001224110524.9987349
AAGACCG500.002260134822.4988615
GGTATCA1459.094947E-1221.7726441
TAAACTG550.00392882620.4535085
ATTAAAC550.00392882620.4535083
ACAGGTC550.00392882620.4535088
TATCAAC3750.020.3989662
GTATTGG807.4033494E-519.7314591
ATCAACG3650.019.7250293
CAACGCA3700.018.8503975
TCAACGC3700.018.8503974
ACATCGT600.0064978518.7490528
TATACAG600.0064978518.7490525
AACGCAG3750.018.5990586
TTAAACT750.001107149517.9990884
CTTTGCA901.8587052E-417.4991139
ACGCAGA4000.017.4366177
Produced by FastQC (version 0.11.2)