FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_2_1.3410000000966b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_2_1.3410000000966b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259276
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC206.971108E-445.0679931
TGGGGCA352.814961E-432.1417545
ACAACGG300.005140856429.998973
GGTATCA1350.028.3761431
GTATCAA4000.027.6041471
ACGGGGC450.001224238224.99914416
ATTATAC450.001224238224.9991443
TTAGCCT550.003929225720.4538444
ACGGGGT550.003929225720.45384416
CATTATA550.003929225720.4538442
TATCAAC5750.019.5645472
TTATAAG706.965129E-419.2850512
CGGGGGA706.965129E-419.28505117
TCAACGC5950.018.9069144
GTATATA600.00644080918.778331
GTTATAA600.00644080918.778331
GTGTTAT600.00649850618.74935513
ACAGATC600.00649850618.7493558
CAACGCA6000.018.374375
AACGCAG6100.018.073156
Produced by FastQC (version 0.11.2)