FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_1_9.3410000000960e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_1_9.3410000000960e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549516
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG11070.2014500032756098No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC10310.187619650747203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.18216030106493714No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC7860.14303496167536522No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7810.14212507006165426No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7450.13557385044293524No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA7380.13430000218373986No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6920.1259289993375989No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA6260.11391843003661403No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT6160.1120986468091921No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6050.11009688525902796No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA5910.10754918874063722No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT5910.10754918874063722No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC5690.10354566564030894No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG5660.10299973067208233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA250.002106571335.9985437
TATGCCG653.6743586E-1034.61398743
CTGTGTA352.8191434E-432.1415569
CGTATGC803.3887773E-928.12386141
GTATGCC803.3887773E-928.12386142
TCGTATG803.3887773E-928.12386140
GCCTCGA2200.026.58983232
CGTCGGG2350.025.85001614
GCCCACG901.1887096E-824.9989918
CCTCGAT2350.024.89260933
GGGGCAA2450.024.79491618
TGAGTAG2450.022.95825639
TACCCGG2550.022.9402486
ATACCCG2650.022.9236015
TCGCGCG2750.022.9081639
ACCTATA2700.022.540111
CTCGTAT1003.6372512E-822.4990939
GTAGGCC500.00226364922.499093
ACTGTAT1003.6372512E-822.4990932
ATGCCGT1003.6372512E-822.4990944
Produced by FastQC (version 0.11.2)