FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_1_7.341000000095c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_1_7.341000000095c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445400
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.3053435114503817No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12160.2730130220026942No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11020.24741805118994165No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10430.234171531207903No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG9680.2173327346205658No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC9290.2085765603951504No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9200.20655590480466995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.18545127974854062No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6670.14975303098338574No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC6610.14840592725639876No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA6120.13740458015267176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.1317916479568927No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA5320.11944319712617871No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4930.11068702290076336No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA4920.11046250561293221No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT4760.1068702290076336No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG4730.10619667714414009No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC4500.10103277952402336No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4490.1008082622361922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCT207.030098E-444.998099
GCACTCC250.002106164635.99847431
CCTAGTG300.005144980329.9987282
CGCATAA300.005144980329.9987289
TAGGTCT406.161696E-428.12380644
CGATCGT406.161696E-428.12380628
CGTCGGG2300.023.47726614
ACCTATA2400.022.5420671
GTATAAT500.002238020822.5420651
GCGGGGG1003.6332494E-822.49904625
AGCCAGG2450.022.03988326
GCCTCGA2450.022.03988332
GGGGCAA2350.022.02034418
GGGCCTC1156.7448127E-921.52082629
GGCGGGG1056.089431E-821.42766224
ATACCCG2450.021.1215535
CGATGAG2350.021.06293936
TCGGCAG1401.364242E-1020.8919734
CGCGCGC2050.020.85277410
TCGCGCG2050.020.8527749
Produced by FastQC (version 0.11.2)