FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_1_6.341000000095a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_1_6.341000000095a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711005
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG20450.2876210434525777No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC19400.2728532148156483No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC15800.22222065948903313No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA12670.17819846555228164No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT12300.17299456403260174No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA11640.16371192888938896No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA11390.16019577921392958No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT10920.15358541782406593No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG10830.15231960394090055No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAAC10330.1452873045899818No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC10130.14247438484961428No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAAG9970.14022404905732028No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC9930.13966146510924676No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAT9780.13755177530397114No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA9580.13473885556360363No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG9480.13333239569341987No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTAT9360.13164464384919938No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA9270.13037882996603398No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCG8470.11912715100456396No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT8380.11786133712139858No Hit
CTTGTGGAGACGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTAACGG8330.1171581071863067No Hit
CTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCGT8320.11701746119928834No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGG8130.1143451874459392No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATG7950.11181355967960845No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTT7900.11111032974451657No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTG7720.10857870197818582No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG7540.10604707421185504No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAA7430.10449996835465293No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGC7420.10435932236763454No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGG7400.1040780303935978No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTC7310.10281221651043242No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGA7220.10154640262726705No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGC7210.10140575664024866No Hit
GCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATCG7190.10112446466621192No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACG7160.10070252670515678No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC7150.1005618807181384No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTT7140.10042123473112004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG352.819974E-432.14155237
CTAGTAT300.005146975629.9987813
GGGCAAC300.005146975629.99878117
GACGCGC406.165278E-428.12385638
CAGGCGA751.6967278E-623.99902537
CGCGCAT500.002264301322.49908640
TATATAA500.002264301322.4990862
ACGCGCA500.002264301322.49908639
TAGGTCT802.9731564E-622.49908444
CCTCGAT4800.022.03035533
GCCTCGA4950.021.36276832
TAAGGTA955.527763E-721.3149225
AGGCGAT855.029522E-621.1756138
TAGGACA654.2372025E-420.7683854
GTGACGC654.2372025E-420.76838536
ACCTATA5000.020.7370741
ACGTTAG2950.020.6301061
TTCTAGA550.00393601120.4537142
TATTAAA550.00393601120.4537142
CGTCAAT550.00393601120.45371419
Produced by FastQC (version 0.11.2)