FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_1_5.34100000009586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_1_5.34100000009586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595557
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27500.4617526114209051No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA19960.3351484408713188No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG16610.2788985772982267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16270.27318963592065915No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15110.2537120712207228No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC14870.24968223024832217No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG14540.24414119891127128No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAA12490.20971964060534928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.2095517305648326No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11630.1952793771209137No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATT11230.18856297550024598No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10630.17848837306924442No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA9550.1603540886934416No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA9550.1603540886934416No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8960.15044739630295673No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT8830.14826456577623973No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC8760.1470891954926229No Hit
GCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG8620.14473845492538917No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7940.13332057217025406No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC7910.132816842048704No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG7210.1210631392125355No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA6960.11686538819961818No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT6740.11317136730825093No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA6720.11283554722721756No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA6630.11132435686256731No Hit
TGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAA6610.11098853678153393No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6530.10964525645740038No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC6520.10947734641688368No Hit
ATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACT6490.10897361629533361No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATG6230.10460795524189961No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA6170.10360049499879943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1250.041.39814841
TCGTATG1250.041.39814840
GTATGCC1350.038.3316242
GACCTCT1350.038.3316226
TATGCCG1400.036.9626343
CCCACGA1400.036.9626319
AGACCTC1400.036.9626325
ATGCCGT1400.036.9626344
TAGCCAT250.002106757835.998394
CTTATCT1450.035.68805734
TATCTCG1450.035.68805736
CTCGTAT1550.033.385639
TACTTAT1600.032.342332
TCTAACT352.8194973E-432.1414228
TTATCTC1650.031.36223435
AAGTTGC300.005146417329.99865717
CTAATGG300.005146417329.9986572
GCCCACG1750.029.57010518
CCTCTAC2000.026.99879328
CACGAGA2100.026.78451521
Produced by FastQC (version 0.11.2)