FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_1_4.3410000000956c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_1_4.3410000000956c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919269
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG855.456968E-1231.76351718
GTATCAA23250.022.2948251
GGTATCA11000.021.7176251
TATGCCG1257.530616E-1021.5991943
ATGCCGT1257.530616E-1021.5991944
CGTATGC1301.2278178E-920.76845241
GCGCGCT550.00393680220.4537815
TTAAACG550.00393680220.453783
CGCCACG550.00393680220.4537820
GTATGCC1452.1827873E-1020.17165842
GACCTAT1403.086825E-919.28499226
GTATTAA1202.4523797E-718.7809641
CGCGCGC600.006510936718.74929614
TACACAG1354.271169E-818.3326455
CTCGTAT1507.2632247E-917.99932539
CCCACGA1507.2632247E-917.99932519
TATACGG1153.4483764E-617.6080365
CCTATTC1802.0736479E-1017.49934628
TATAACT901.8656984E-417.4993464
AGCCCAC1551.08957465E-817.41870117
Produced by FastQC (version 0.11.2)