FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_1_3.34100000009543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_1_3.34100000009543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251847
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCG20810.8262953301012124No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA19160.7607793620730047No Hit
GTATCAACGCAGAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCA16470.6539684808633813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15570.6182324983025408No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAA14320.5685991891902623No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC14220.5646285244612801No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13100.5201570794966786No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTC12820.5090392182555281No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11080.43994965197123653No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGGA10860.4312141895674755No Hit
CTGCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCT10750.426846458365595No Hit
GAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGC10200.40500780235619244No Hit
TATCAACGCAGAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCAT9190.3649040885934714No Hit
CATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGC9160.36371288917477673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.3613304903373874No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAGC8900.3533891608794228No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGG8520.3383006349092902No Hit
CGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGG8510.33790356843639197No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGT8390.3331387707616132No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8340.3311534383971221No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATAT8140.3232121089391575No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTC7860.3120942476980071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.2902555916886046No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGA7190.2854907940138259No Hit
GGTATCAACGCAGAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCC6890.273578799826879No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6580.26126973916703394No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACG6200.24618121319690128No Hit
GTATCAACGCAGAGTACGGGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGG5750.228313221916481No Hit
CATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGC5720.22712202249778635No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5490.21798949362112713No Hit
GCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAG5380.2136217624192466No Hit
ACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTT5120.20329803412389266No Hit
CCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGA4930.19575377113882636No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4800.1905919069911494No Hit
GTATCAACGCAGAGTACGGGCAGGCCTCGATGAGTAGGAGGGCGCGGCGGT4640.18423884342477775No Hit
GAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACT4630.18384177695187953No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4480.1778857798584061No Hit
CCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACC4350.17272391571072915No Hit
ATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGT4320.17153271629203445No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4250.16875325098174684No Hit
ACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCC4210.16716498509015396No Hit
GTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCG4000.15882658915929115No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.15604712384900354No Hit
TGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCT3910.15525299090320713No Hit
GGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA3900.15485592443030888No Hit
TCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCC3890.15445885795741066No Hit
TCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGG3710.14731166144524255No Hit
AATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACT3590.1425468637704638No Hit
GACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGC3490.13857619904148155No Hit
ACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT3420.13579673373119394No Hit
TATCAACGCAGAGTACGGGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGT3390.13460553431249925No Hit
GGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGA3390.13460553431249925No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.13262020194800814No Hit
ACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTG3330.1322231354751099No Hit
GTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGC3330.1322231354751099No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG3300.1310319360564152No Hit
CTCATAGGCAGTGGCTTGGTTAAGGGAACCCACCGGAGCCGTAGCGAAAGC3200.12706127132743295No Hit
CCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAA3180.12626713838163647No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA3040.12070820776106128No Hit
CTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCC2940.116737543032079No Hit
GCCCGGGCTCGCGCCCTAGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCA2900.11514927714048609No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2900.11514927714048609No Hit
TTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCC2870.1139580777217914No Hit
GCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACC2840.11276687830309672No Hit
GTACGGGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACC2830.1123698118301985No Hit
GAGTACGGGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAA2820.11197274535730026No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATT2810.11157567888440204No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2810.11157567888440204No Hit
GCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG2810.11157567888440204No Hit
GTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATA2780.11038447946570736No Hit
AACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGG2770.10998741299280913No Hit
TATCAACGCAGAGTACGGGCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTC2740.10879621357411445No Hit
CTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGAT2730.1083991471012162No Hit
GCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTG2710.10760501415541977No Hit
GGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGTCGG2690.1068108812096233No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.10363434942643748No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2540.10085488411614989No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAT253.8829123E-544.99841332
CTCGTAT301.137628E-437.49867639
TATGCCG301.137628E-437.49867643
CTTATCT301.137628E-437.49867634
TATCTCG301.137628E-437.49867636
GTGTCTT301.137628E-437.49867630
ACGAGAC301.137628E-437.49867622
AGTGTCT301.137628E-437.49867629
TCTTATC352.8147484E-432.14172433
TGTCTTA352.8147484E-432.14172431
GTATGCC352.8147484E-432.14172442
TCGTATG352.8147484E-432.14172440
ATGCCGT352.8147484E-432.14172444
TGCCGTC352.8147484E-432.14172445
AAGAGCC300.005140603529.99894123
CCTCCCC300.005140603529.99894144
CGTATGC406.1539427E-428.12400641
CACGAGA406.1539427E-428.12400621
CCACGAG406.1539427E-428.12400620
AGGCAGT1004.5474735E-1126.9990486
Produced by FastQC (version 0.11.2)