##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb3_1_11.34100000009642.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 164177 Sequences flagged as poor quality 0 Sequence length 51 %GC 31 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.96188260231336 38.0 38.0 38.0 32.0 38.0 2 36.93291995833765 38.0 38.0 38.0 32.0 38.0 3 36.8431266255322 38.0 38.0 38.0 32.0 38.0 4 36.86346443168044 38.0 38.0 38.0 32.0 38.0 5 36.83659708728994 38.0 38.0 38.0 32.0 38.0 6 38.864384170742554 40.0 38.0 40.0 38.0 40.0 7 38.767988207848845 40.0 38.0 40.0 38.0 40.0 8 38.73914738361646 40.0 38.0 40.0 38.0 40.0 9 38.706450964507816 40.0 38.0 40.0 38.0 40.0 10 38.638432910821855 40.0 38.0 40.0 38.0 40.0 11 38.690047936069 40.0 38.0 40.0 38.0 40.0 12 38.66129847664411 40.0 38.0 40.0 38.0 40.0 13 38.6043477466393 40.0 38.0 40.0 38.0 40.0 14 38.59407225128977 40.0 38.0 40.0 38.0 40.0 15 38.67682440293098 40.0 38.0 40.0 38.0 40.0 16 38.77366500788783 40.0 38.0 40.0 38.0 40.0 17 38.760880025825784 40.0 38.0 40.0 38.0 40.0 18 38.76923686021794 40.0 38.0 40.0 38.0 40.0 19 38.781169104076696 40.0 38.0 40.0 38.0 40.0 20 38.74408108322116 40.0 38.0 40.0 38.0 40.0 21 38.74352071240186 40.0 38.0 40.0 38.0 40.0 22 38.69262442364034 40.0 38.0 40.0 38.0 40.0 23 38.71949785901801 40.0 38.0 40.0 38.0 40.0 24 38.765137625855026 40.0 38.0 40.0 38.0 40.0 25 38.744921639450105 40.0 38.0 40.0 38.0 40.0 26 38.71170139544516 40.0 38.0 40.0 38.0 40.0 27 38.65776570408766 40.0 38.0 40.0 38.0 40.0 28 38.61208330034049 40.0 38.0 40.0 38.0 40.0 29 38.46191610274277 40.0 38.0 40.0 38.0 40.0 30 38.38727105501989 40.0 38.0 40.0 38.0 40.0 31 38.20747120485817 40.0 38.0 40.0 38.0 40.0 32 37.95155228807933 40.0 38.0 40.0 38.0 40.0 33 37.637647173477404 40.0 38.0 40.0 32.0 40.0 34 37.25789239662072 40.0 38.0 40.0 32.0 40.0 35 36.88717664471881 40.0 38.0 40.0 27.0 40.0 36 36.50497938200844 40.0 38.0 40.0 27.0 40.0 37 36.22195557233961 40.0 38.0 40.0 27.0 40.0 38 35.88344896057304 40.0 38.0 40.0 13.0 40.0 39 35.49482570640224 40.0 38.0 40.0 13.0 40.0 40 35.05214494113061 40.0 38.0 40.0 13.0 40.0 41 34.59149576371843 40.0 38.0 40.0 13.0 40.0 42 34.09832680582542 40.0 32.0 40.0 13.0 40.0 43 33.55709995919039 40.0 32.0 40.0 13.0 40.0 44 33.03334206374827 40.0 27.0 40.0 13.0 40.0 45 32.426399556576136 38.0 27.0 40.0 13.0 40.0 46 31.736503895186292 38.0 27.0 40.0 13.0 40.0 47 30.95734481687447 38.0 13.0 40.0 13.0 40.0 48 30.262759095366587 38.0 13.0 40.0 13.0 40.0 49 29.6403942086894 38.0 13.0 40.0 13.0 40.0 50 29.059247032166503 38.0 13.0 40.0 13.0 40.0 51 28.38432910821857 32.0 13.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.38906295464332885 2201 2 0.19334602775428777 2201 3 0.41018882204883056 2201 4 -0.0265414941789075 2201 5 0.19530311329702243 2201 6 0.06613347675366299 2201 7 0.23787646754651348 2201 8 0.2543359760714097 2201 9 0.137360859907659 2201 10 0.4713725219265683 2201 11 0.1403447701381495 2201 12 0.2627021014308255 2201 13 0.22609483545863185 2201 14 0.2148372822331055 2201 15 0.032213266421806 2201 16 0.531470307140431 2201 17 0.45662880784659876 2201 18 0.13838080464962843 2201 19 0.2858020274088986 2201 20 0.1248725202796237 2201 21 0.10817315908421676 2201 22 0.43585223312872046 2201 23 0.2717454142573885 2201 24 0.4260587010679231 2201 25 0.308923302391527 2201 26 0.40220017578559464 2201 27 0.4972426709813007 2201 28 0.5440255556210261 2201 29 0.28940999793810107 2201 30 0.26568863959253264 2201 31 0.12362040489545478 2201 32 0.1211655372381486 2201 33 0.3989652461880411 2201 34 0.8488149785760015 2201 35 0.8743015558896161 2201 36 0.8523732462697708 2201 37 1.0113352755099854 2201 38 1.2583175832678108 2201 39 1.2407454942773342 2201 40 1.5170604822497893 2201 41 1.1209309015604418 2201 42 0.5900945278490823 2201 43 0.5767789889237847 2201 44 0.9275427162361822 2201 45 0.8870934468592679 2201 46 0.9475193481601458 2201 47 0.5152959401599162 2201 48 0.8355422516693416 2201 49 0.8114551521042586 2201 50 0.6416813985590828 2201 51 0.8714716578553521 1101 1 -0.011637359290503468 1101 2 0.18622214463427156 1101 3 0.3229152166395082 1101 4 -0.048903242548867354 1101 5 0.19399908045470937 1101 6 0.10100428090724733 1101 7 0.30537224151415643 1101 8 0.26380228855632026 1101 9 0.5586117654860203 1101 10 0.10349036785750343 1101 11 0.049835231379390166 1101 12 0.14606360831322007 1101 13 0.29634356764892544 1101 14 0.48573803084455136 1101 15 0.31868996287194307 1101 16 0.24332734650290888 1101 17 0.23822745551624536 1101 18 0.13910526733980078 1101 19 0.2014987190292814 1101 20 0.12129850434144629 1101 21 0.27984666789851786 1101 22 0.11136539420093072 1101 23 0.32417236426945806 1101 24 0.12021470203387707 1101 25 -0.06311243776785602 1101 26 0.2588531581570024 1101 27 0.21600625465674028 1101 28 -0.0681937150865366 1101 29 0.3362102877231763 1101 30 0.26981807692650506 1101 31 0.10326300330163463 1101 32 0.19733071473150687 1101 33 0.12405580402431582 1101 34 0.6070376301094456 1101 35 0.8807009763194671 1101 36 0.7721027801987717 1101 37 1.3670223076278347 1101 38 1.5176752263491977 1101 39 1.768878795412327 1101 40 1.0873333631964215 1101 41 1.181206197382707 1101 42 1.3429803042316024 1101 43 0.9037048522418232 1101 44 -0.3673860449326156 1101 45 -0.8718053698516712 1101 46 0.2763046657162924 1101 47 -0.08603224143872978 1101 48 -0.8263268620713013 1101 49 -0.9968761688327135 1101 50 -0.5358361092892601 1101 51 -0.29092872852474727 2202 1 0.09238187808496434 2202 2 -0.18547143145642053 2202 3 -0.1544465147242846 2202 4 0.13085170993024065 2202 5 0.1813115732979469 2202 6 0.08746941316420731 2202 7 0.10360881542919032 2202 8 0.19697531042738348 2202 9 0.09654832795023793 2202 10 0.08862720514136413 2202 11 0.1761835652852639 2202 12 0.30179474308935994 2202 13 0.3879272158797704 2202 14 0.14422881293546652 2202 15 0.1949753172747819 2202 16 0.11869663540706199 2202 17 0.38699649250239077 2202 18 0.35689983965171734 2202 19 0.12132008625704316 2202 20 0.5050612433772912 2202 21 0.48708358533811236 2202 22 0.4926318547528581 2202 23 0.38946493268810656 2202 24 0.10418355580691951 2202 25 0.022631292908883438 2202 26 0.17959059088344276 2202 27 0.035289433404017245 2202 28 0.13157726853879126 2202 29 0.3325002224535041 2202 30 0.402326951311025 2202 31 -0.20174100448491572 2202 32 -0.15308724015226716 2202 33 -0.17055086035218636 2202 34 0.05036752819715673 2202 35 -0.017043212390028373 2202 36 0.2310744966764986 2202 37 -0.2702386565311059 2202 38 0.3519818792748808 2202 39 0.329854405168426 2202 40 0.35894678355597875 2202 41 -0.04194735809651462 2202 42 0.1546601393398106 2202 43 -0.03164382518861686 2202 44 0.6238426279174902 2202 45 0.4139841641000075 2202 46 -0.144169398179276 2202 47 -0.4674819150903957 2202 48 -1.0259833375092953 2202 49 -0.4432977879784836 2202 50 -1.1130947832844527 2202 51 -0.23527774947974578 1102 1 -0.0711949316104139 1102 2 -0.03453792343152884 1102 3 0.09920563070643595 1102 4 0.03765376255606867 1102 5 -0.02403835573360169 1102 6 0.07842912492313303 1102 7 0.26714195223451753 1102 8 0.2391851586640925 1102 9 0.3352425085376325 1102 10 0.2955493582148492 1102 11 0.2656038076698053 1102 12 0.35287018800692493 1102 13 0.26208378319061154 1102 14 0.33724115052690706 1102 15 0.33060147733592515 1102 16 0.4448021054365441 1102 17 0.26897050713280635 1102 18 0.21114156909816728 1102 19 -0.11523412271773736 1102 20 0.5116558497753658 1102 21 -0.05537738201640252 1102 22 -0.19788020988301724 1102 23 0.01549397515999118 1102 24 0.3887342709504935 1102 25 -0.18018560849956344 1102 26 -0.005582462943394262 1102 27 0.4654674004309882 1102 28 0.1105924448680895 1102 29 0.22696468363922406 1102 30 0.25949476439105723 1102 31 0.16974059831014898 1102 32 0.011057317113809972 1102 33 0.20573476034876137 1102 34 0.18162639925294854 1102 35 -0.23569153644287155 1102 36 -0.5397520127677637 1102 37 -0.8616787700806157 1102 38 -1.1212357208998114 1102 39 -0.26549443204087453 1102 40 -0.8646802499629658 1102 41 -1.0160711707398136 1102 42 -0.7751138534541653 1102 43 -0.5220467075993582 1102 44 -0.1282482116938155 1102 45 -0.03765902838825497 1102 46 -0.4025382157357775 1102 47 0.20903298828333305 1102 48 -0.42473866014277206 1102 49 0.09331101210886317 1102 50 -0.17860411836469936 1102 51 0.38496463506005085 2203 1 0.007874835831444216 2203 2 0.26523279389569154 2203 3 0.21175066837430734 2203 4 0.11676720288798492 2203 5 0.35032565780499425 2203 6 0.38793889673227966 2203 7 0.11299848679069413 2203 8 -0.192696051074968 2203 9 -0.1241089490450733 2203 10 0.2623361253291634 2203 11 0.34050281411155936 2203 12 0.12808582290156778 2203 13 0.3879272158797704 2203 14 -0.0717336283790857 2203 15 -0.24164440103508156 2203 16 0.029494757472789956 2203 17 0.15694954414558282 2203 18 0.2630031260366934 2203 19 0.05559238672652356 2203 20 0.035577675302171485 2203 21 -0.16080373860555142 2203 22 -0.26323668984807824 2203 23 0.09838512048153092 2203 24 0.057235198999407544 2203 25 -0.04309640662162906 2203 26 0.29226664722147433 2203 27 0.1996086822303127 2203 28 0.25364299623832665 2203 29 0.47334529287604 2203 30 0.13472131750820893 2203 31 0.23487871382494063 2203 32 -0.41130320259357944 2203 33 -0.36773395894373806 2203 34 0.31327832631922803 2203 35 0.6120647688306278 2203 36 0.4799007877563142 2203 37 0.03023082703697355 2203 38 -0.32407445875328733 2203 39 -0.759347472765846 2203 40 -0.19504382677266108 2203 41 -0.12175956466928284 2203 42 0.0889324398092981 2203 43 0.5270416208207749 2203 44 0.3280679800301698 2203 45 0.4374583425037599 2203 46 0.29245032013058037 2203 47 0.6498889769283842 2203 48 0.13833591131699663 2203 49 0.5003641838525041 2203 50 0.5582667190629671 2203 51 1.0464123913653225 1103 1 0.23796333140666803 1103 2 0.2788187343169781 1103 3 0.3874954022643706 1103 4 0.21384227871165962 1103 5 0.37610570683070677 1103 6 0.2882047989301242 1103 7 0.6214741193470914 1103 8 0.28763134100613286 1103 9 -0.009313984567945965 1103 10 0.45665246585016206 1103 11 0.43930209949940036 1103 12 0.3676263106659121 1103 13 0.30666085666252485 1103 14 0.255886962422359 1103 15 0.39425655039622143 1103 16 0.3653001503017421 1103 17 0.45141545465287436 1103 18 0.5551423923128169 1103 19 0.2536895241231747 1103 20 0.5192911656839883 1103 21 0.4380163203915046 1103 22 0.26258490639605014 1103 23 0.24953390107067008 1103 24 0.26860667267924754 1103 25 0.09592058619070087 1103 26 0.10092093281705417 1103 27 0.21304920738715083 1103 28 0.2473901398670577 1103 29 0.20424395921033067 1103 30 -0.12912429302816264 1103 31 -0.1126014473782675 1103 32 -0.8438305198896856 1103 33 0.11201177207956903 1103 34 -0.14461356781846035 1103 35 -0.4155433578052907 1103 36 0.43802119159527564 1103 37 0.30039161477223075 1103 38 0.41471817907960684 1103 39 0.7944561750799295 1103 40 1.217270768765168 1103 41 1.5671764110671447 1103 42 1.1464337900938943 1103 43 1.4845595899927346 1103 44 1.3581481661617048 1103 45 1.7782701751515688 1103 46 1.5369349268861576 1103 47 1.6074277197095554 1103 48 0.7759610723124659 1103 49 0.37489158424304847 1103 50 0.36235081063303554 1103 51 -0.7848851282657385 2204 1 0.15602298397958947 2204 2 0.03576545488218841 2204 3 0.3518422466505484 2204 4 -0.04842145995326064 2204 5 -0.05018439841711597 2204 6 0.2637865753968569 2204 7 -0.14579708499392297 2204 8 0.08227062138779218 2204 9 0.11213981928507621 2204 10 0.3862566029272614 2204 11 0.38350982738461425 2204 12 0.3705943721393794 2204 13 0.41737137051954676 2204 14 0.035030412657256704 2204 15 0.3241702398969295 2204 16 0.19972602160076747 2204 17 0.01401788008715954 2204 18 -0.09606486213927212 2204 19 0.0024422099456771207 2204 20 0.19752052582092006 2204 21 0.25071889513930756 2204 22 0.24200877470880755 2204 23 0.3439655992388495 2204 24 0.08227432263007728 2204 25 0.059320564339941484 2204 26 0.16649141972480663 2204 27 0.11069660896769307 2204 28 -0.05227481515679244 2204 29 0.32282074642035496 2204 30 0.17338126070649196 2204 31 -0.17281849825411655 2204 32 -0.08162140812305552 2204 33 -0.3042087749177682 2204 34 -0.6726371666385234 2204 35 -0.667828583050202 2204 36 -0.8303061327792491 2204 37 -0.6298862540503762 2204 38 -0.846070633331621 2204 39 -0.6824912738439224 2204 40 -0.48137084236415006 2204 41 -0.4269238839181071 2204 42 -0.1890482012386414 2204 43 -0.6553034785822263 2204 44 -0.328803288734683 2204 45 -0.5183174646022479 2204 46 -0.31799424023573053 2204 47 -0.30385819747606746 2204 48 0.015226886799521111 2204 49 -0.6590863464900458 2204 50 -0.7232495389791147 2204 51 -0.9419374608342928 2205 1 0.27210247810786825 2205 2 0.011425396666787435 2205 3 -0.22830429555561693 2205 4 0.1486577330608938 2205 5 -0.1377082366184581 2205 6 0.24571972806172226 2205 7 0.22407142850602213 2205 8 0.0963226137595683 2205 9 0.23991257849414893 2205 10 0.023815571109530254 2205 11 -0.02821354910841478 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-0.3957670777020539 1213 43 -0.13222515248759237 1213 44 -0.2591636275283875 1213 45 -0.18890556578438122 1213 46 -0.06009152373036741 1213 47 0.20939374329203275 1213 48 -0.2647566978932048 1213 49 0.3003199665635421 1213 50 0.780939784231446 1213 51 0.5598921619556236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 9.0 15 30.0 16 52.0 17 33.0 18 20.0 19 24.0 20 33.0 21 70.0 22 125.0 23 203.0 24 338.0 25 554.0 26 935.0 27 1356.0 28 1988.0 29 2806.0 30 4314.0 31 6055.0 32 7473.0 33 9301.0 34 10790.0 35 11098.0 36 12650.0 37 15756.0 38 18080.0 39 60084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 62.021339547708955 13.332031893412069 14.93341304652851 9.713215512350462 2 13.624320093557563 27.137175121972017 43.71988768219665 15.518617102273765 3 23.72317681526645 38.722841810972305 26.891708339170528 10.662273034590717 4 10.168903074121223 16.795897111044784 38.429865328273756 34.60533448656024 5 13.410526444020782 38.488338805070136 25.762439318540352 22.338695432368723 6 23.44177320818385 39.75100044464207 12.522460515175696 24.284765831998392 7 22.476960841043507 38.292818117032226 22.435542128312736 16.79467891361153 8 31.069516436528865 22.009782125389062 18.070131626232662 28.850569811849407 9 31.127989913325255 13.994652113267996 24.12761836310811 30.749739610298644 10 24.351766690827585 26.296618893023993 22.088355859834202 27.263258556314224 11 24.656925147858715 29.72462647021203 25.17283176084348 20.44561662108578 12 33.84517928820724 28.680631269909913 28.92914354629455 8.545045895588297 13 30.09739488478898 27.776728774432474 30.122977030887395 12.002899309891154 14 25.47129013199169 22.5250796396572 41.17811873770382 10.82551149064729 15 16.56017590770936 27.38081460862362 40.96493418688367 15.094075296783348 16 9.958154918167587 34.404941008789294 37.2695322730955 18.36737179994762 17 13.654775029389013 25.457280861509226 33.14227936921737 27.74566473988439 18 22.107237920049702 19.709825371397944 40.67317590161837 17.50976080693398 19 27.27909512294658 19.31452030430572 43.66750519256656 9.738879380181146 20 22.922821101615938 17.219220719101944 43.39219257265025 16.465765606631866 21 22.235757749258422 20.463280483868022 46.10085456549943 11.200107201374127 22 15.606936416184972 23.543492693860895 47.72836633633213 13.121204553622007 23 17.089482692459967 25.924459577163674 47.90135037185477 9.084707358521596 24 17.45433282372074 26.406865760733844 47.97687861271676 8.161922802828654 25 12.345821887353283 29.281811703222743 49.54043501830341 8.831931391120559 26 10.243213117549962 32.16163043544467 48.88930849022701 8.705847956778355 27 8.540173105855265 31.740134123537402 48.94900016445665 10.77069260615068 28 7.304311809815017 32.76463816490739 46.61615207976757 13.31489794551003 29 8.526163835372799 34.913538437174516 43.7606972962108 12.799600431241892 30 9.275355256826474 33.914616541902944 43.73450605139575 13.07552214987483 31 11.582012096700513 31.179154205522092 42.554072738568735 14.68476095920866 32 12.979893651364078 31.42827558062335 42.39205247994543 13.199778288067145 33 11.31400866138375 33.09903336033671 42.20566827265695 13.38128970562259 34 9.948409338701524 36.246246429158774 41.93401024504041 11.87133398709929 35 10.340668912210601 38.10582481102712 41.43637659355452 10.117129683207757 36 9.625587018888151 39.356913574983096 41.32064783739501 9.696851568733745 37 8.676002119663535 40.80108663211047 40.93569744848548 9.587213799740525 38 9.470875944864385 41.114772471174405 40.44171838929935 8.972633194661858 39 8.375107353648806 42.035729730717456 39.38371391851477 10.205448997118962 40 8.575500831419749 42.284242007102094 39.22656644962449 9.91369071185367 41 8.563318857087168 41.69280715325533 38.518793740901586 11.225080248755916 42 9.756543242963387 41.341966292477025 36.458212782545665 12.443277682013925 43 8.883095683317395 40.96493418688367 35.30823440555011 14.84373572424883 44 8.902586842249523 41.023407663680054 34.47620555863489 15.597799935435535 45 8.54991868532133 39.45802396194351 34.22282049251722 17.769236860217934 46 9.177290363449204 39.35934996984961 33.19709825371398 18.266261412987202 47 10.089720240959451 37.630727812056506 31.949664082057776 20.32988786492627 48 9.957545819450958 37.045383945376024 31.00251557769968 21.994554657473337 49 10.989968144137121 35.91976951704563 29.720362779195625 23.36989955962163 50 10.926012778891076 35.552482990918335 30.012121064460917 23.50938316572967 51 11.435828404709552 35.81256814291892 28.93523453346084 23.816368918910687 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 9.0 2 11.0 3 49.0 4 87.0 5 133.0 6 179.0 7 235.0 8 291.0 9 523.5 10 756.0 11 1616.0 12 2476.0 13 4721.0 14 6966.0 15 9618.5 16 12271.0 17 13014.5 18 13758.0 19 12692.0 20 11626.0 21 10872.5 22 10119.0 23 10506.5 24 10894.0 25 11197.0 26 8969.5 27 6439.0 28 5009.0 29 3579.0 30 3446.5 31 3314.0 32 5564.0 33 7814.0 34 8235.0 35 8656.0 36 7215.0 37 5774.0 38 4329.5 39 2885.0 40 2392.0 41 1899.0 42 2126.5 43 2354.0 44 2600.5 45 2847.0 46 3437.0 47 4027.0 48 4996.5 49 5966.0 50 5250.0 51 4534.0 52 4455.0 53 4376.0 54 4765.0 55 5154.0 56 4785.0 57 4416.0 58 3699.5 59 2983.0 60 2453.0 61 1923.0 62 1563.5 63 1204.0 64 1047.0 65 890.0 66 766.5 67 643.0 68 549.0 69 455.0 70 404.0 71 353.0 72 323.5 73 294.0 74 240.5 75 149.5 76 112.0 77 93.5 78 75.0 79 60.5 80 46.0 81 35.5 82 25.0 83 17.5 84 10.0 85 5.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15592927145702504 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 164177.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.86808212200249 #Duplication Level Percentage of deduplicated Percentage of total 1 86.30885337913845 36.99895014512779 2 6.895443999963089 5.911889193161742 3 2.3166864312618434 2.9793571255878475 4 1.0429049600684985 1.7882934189464053 5 0.7036561705976927 1.5082195253417843 6 0.4560129950704778 1.1729041512828937 7 0.32400008583009793 0.9722483600830347 8 0.20244139665813848 0.6942619533467169 9 0.1846634934280083 0.7124552821086957 >10 1.248173138611775 10.366136770160523 >50 0.16253387939069686 4.650712293799959 >100 0.12107863103124267 10.011174057862386 >500 0.01750509858266252 4.828888373135925 >1k 0.014587582152218765 14.200258634702223 >5k 0.0014587582152218764 3.204250715352043 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 5124 3.1210218240070167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4158 2.532632463743399 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3461 2.108090658252983 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 2894 1.7627316859243378 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2344 1.4277273917783857 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 2248 1.369253914982001 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2216 1.349762756049873 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1886 1.1487601795623017 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 1191 0.7254365715051438 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 1174 0.7150818933224508 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1136 0.6919361420905487 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 895 0.5451433513829586 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 885 0.5390523642166686 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 743 0.45256034645535004 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 640 0.3898231786425626 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 628 0.38251399404301456 No Hit TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 616 0.3752048094434665 No Hit GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 609 0.3709411184270635 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 565 0.3441407748953873 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 549 0.3343951954293233 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.3295224056962912 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 540 0.3289133069796622 No Hit GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA 511 0.31124944419742107 No Hit GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA 474 0.2887127916821479 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 0.28018540964934185 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 459 0.27957631093271285 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 442 0.26922163275001976 No Hit GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA 378 0.23023931488576352 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 367 0.2235392290028445 No Hit TCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 358 0.21805734055318346 No Hit ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA 342 0.20831176108711938 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 342 0.20831176108711938 No Hit ATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 329 0.20039347777094235 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 317 0.19308429317139428 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT 316 0.1924751944547653 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 299 0.1821205162720722 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 296 0.1802932201221852 No Hit TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 272 0.1656748509230891 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT 261 0.15897476504017008 No Hit GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 235 0.14313819840781594 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA 225 0.1370472112415259 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT 225 0.1370472112415259 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 224 0.13643811252489693 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 223 0.1358290138082679 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT 215 0.13095622407523588 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 214 0.13034712535860687 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 211 0.12851982920871985 No Hit GTATCTCCCCATGGCCTTTCGCCTCTGAAAGCGTCCTTCCTTCTCAATGCC 209 0.12730163177546186 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 208 0.12669253305883285 No Hit GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA 207 0.12608343434220384 No Hit GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 204 0.12425613819231683 No Hit CAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 204 0.12425613819231683 No Hit GGGAGATACCGTCTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCAA 204 0.12425613819231683 No Hit ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 203 0.12364703947568781 No Hit GTCCAAAGAGCGCAGACTAGCCACGGAGCTTGGATACGGTTTCCCGATCGG 202 0.12303794075905883 No Hit GGTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA 199 0.12121064460917182 No Hit GTATCAACGCAGAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAG 199 0.12121064460917182 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT 192 0.11694695359276878 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 190 0.11572875615951078 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT 186 0.11329236129299476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 185 0.11268326257636577 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177 0.10781047284333371 No Hit GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA 170 0.1035467818269307 No Hit GAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA 170 0.1035467818269307 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 6.09098716629004E-4 9 0.0 0.0 6.09098716629004E-4 10 0.0 0.0 6.09098716629004E-4 11 0.0 0.0 0.001218197433258008 12 0.0 0.0 0.001218197433258008 13 0.0 0.0 0.001218197433258008 14 0.0 0.0 0.001218197433258008 15 0.0 0.0 0.001218197433258008 16 0.0 0.0 0.0036545922997740245 17 0.0 0.0 0.0036545922997740245 18 0.0 0.0 0.004872789733032032 19 0.0 0.0 0.005481888449661037 20 0.0 0.0 0.007309184599548049 21 0.0 0.0 0.032282231981337214 22 0.0 0.0 0.05055519348020734 23 0.0 0.0 0.09745579466064065 24 0.0 0.0 0.15592927145702504 25 0.0 0.0 0.18516600985521722 26 0.0 0.0 0.21135725467026442 27 0.0 0.0 0.26130334943384276 28 0.0 0.0 0.5092065271018474 29 0.0 0.0 1.1317054154966895 30 0.0 0.0 1.9777435328943762 31 0.0 0.0 2.528977871443625 32 0.0 0.0 2.8840824232383344 33 0.0 0.0 3.306187833862234 34 0.0 0.0 3.728293244486134 35 0.0 0.0 4.06086114376557 36 0.0 0.0 4.328255480365703 37 0.0 0.0 4.668132564244687 38 0.0 0.0 5.123129305566553 39 0.0 0.0 5.710300468396913 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 20 7.0167787E-4 44.998478 6 TGCCGTC 45 3.8016879E-10 44.998478 45 CGTATGC 50 1.0695658E-9 40.49863 41 GACGTCT 50 1.0695658E-9 40.49863 26 CGTCTTA 50 1.0695658E-9 40.49863 28 CACGAGA 50 1.0695658E-9 40.49863 21 TATGCCG 50 1.0695658E-9 40.49863 43 AGACGTC 50 1.0695658E-9 40.49863 25 GTATGCC 50 1.0695658E-9 40.49863 42 TCGTATG 50 1.0695658E-9 40.49863 40 ACGTCTT 50 1.0695658E-9 40.49863 27 CGAGACG 50 1.0695658E-9 40.49863 23 GTCTTAG 50 1.0695658E-9 40.49863 29 ATGCCGT 50 1.0695658E-9 40.49863 44 ACGAGAC 50 1.0695658E-9 40.49863 22 TAGTATC 50 1.0695658E-9 40.49863 33 ATCTCGT 55 2.7193892E-9 36.816936 37 CTCGTAT 55 2.7193892E-9 36.816936 39 TCTTAGT 55 2.7193892E-9 36.816936 30 GAGACGT 55 2.7193892E-9 36.816936 24 >>END_MODULE