FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_1_11.34100000009642.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_1_11.34100000009642.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164177
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA51243.1210218240070167No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41582.532632463743399No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34612.108090658252983No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA28941.7627316859243378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23441.4277273917783857No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA22481.369253914982001No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22161.349762756049873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18861.1487601795623017No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11910.7254365715051438No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA11740.7150818933224508No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11360.6919361420905487No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA8950.5451433513829586No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA8850.5390523642166686No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7430.45256034645535004No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC6400.3898231786425626No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6280.38251399404301456No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6160.3752048094434665No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA6090.3709411184270635No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5650.3441407748953873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5490.3343951954293233No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.3295224056962912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT5400.3289133069796622No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA5110.31124944419742107No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA4740.2887127916821479No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.28018540964934185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT4590.27957631093271285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4420.26922163275001976No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA3780.23023931488576352No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3670.2235392290028445No Hit
TCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3580.21805734055318346No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA3420.20831176108711938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3420.20831176108711938No Hit
ATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3290.20039347777094235No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3170.19308429317139428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT3160.1924751944547653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT2990.1821205162720722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT2960.1802932201221852No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2720.1656748509230891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT2610.15897476504017008No Hit
GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2350.14313819840781594No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA2250.1370472112415259No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2250.1370472112415259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT2240.13643811252489693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2230.1358290138082679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT2150.13095622407523588No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2140.13034712535860687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2110.12851982920871985No Hit
GTATCTCCCCATGGCCTTTCGCCTCTGAAAGCGTCCTTCCTTCTCAATGCC2090.12730163177546186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2080.12669253305883285No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA2070.12608343434220384No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2040.12425613819231683No Hit
CAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA2040.12425613819231683No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCAA2040.12425613819231683No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2030.12364703947568781No Hit
GTCCAAAGAGCGCAGACTAGCCACGGAGCTTGGATACGGTTTCCCGATCGG2020.12303794075905883No Hit
GGTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1990.12121064460917182No Hit
GTATCAACGCAGAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAG1990.12121064460917182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT1920.11694695359276878No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1900.11572875615951078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1860.11329236129299476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1850.11268326257636577No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.10781047284333371No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA1700.1035467818269307No Hit
GAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.1035467818269307No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT207.0167787E-444.9984786
TGCCGTC453.8016879E-1044.99847845
CGTATGC501.0695658E-940.4986341
GACGTCT501.0695658E-940.4986326
CGTCTTA501.0695658E-940.4986328
CACGAGA501.0695658E-940.4986321
TATGCCG501.0695658E-940.4986343
AGACGTC501.0695658E-940.4986325
GTATGCC501.0695658E-940.4986342
TCGTATG501.0695658E-940.4986340
ACGTCTT501.0695658E-940.4986327
CGAGACG501.0695658E-940.4986323
GTCTTAG501.0695658E-940.4986329
ATGCCGT501.0695658E-940.4986344
ACGAGAC501.0695658E-940.4986322
TAGTATC501.0695658E-940.4986333
ATCTCGT552.7193892E-936.81693637
CTCGTAT552.7193892E-936.81693639
TCTTAGT552.7193892E-936.81693630
GAGACGT552.7193892E-936.81693624
Produced by FastQC (version 0.11.2)